Antifungal activity of the piroctone olamine in experimental intra-abdominal candidiasis
Tóm tắt
This work aimed to evaluate the antifungal activity of piroctone olamine in the treatment of intra-abdominal candidiasis in an experimental model using Swiss mice. The mice (n = 6) were infected by intraperitoneal injection of 0.2 ml of C. albicans (107cells/ml in saline). The animals were observed daily for clinical signs and mortality for 14 days. The treatment with piroctone olamine (0.5 mg/kg) was performed 72 h after infection by intraperitoneal administration. For comparison, a group of animals (n = 6) was treated with amphotericin B (0.5 mg/kg). The mycological diagnosis was made by collecting the liver, spleen and kidneys. Data regarding the fungal growth and mortality were analyzed statistically by Student’s t test and analysis of variance (ANOVA), with level of significance set at P < 0.05. The difference in fungal growth scoring between the control group and the treatment groups (piroctone olamine and amphotericin B) was statistically significant (P < 0.05). The difference in fungal growth scoring between the treatment groups (piroctone olamine and amphotericin B) was not statistically significant (P < 0.05).
Tài liệu tham khảo
Allgood GS, Miller JM, Schardein JL (1991) The effect of piroctone olamine on reproduction of male and female rats. Fundam Appl Toxicol 16:31–40
Barnett JA, Payne RW, Yarrow D (1983) Yeasts: characteristics and identification. Cambridge University Press, Cambridge, p 811
Bassetti M, Marchetti M, Chakrabarti A, Colizza S, Montero JG, Kett DH et al (2013) A research agenda on the management of intra-abdominal candidiasis: results from a consensus of multinational experts. Intensive Care Med 39:2092–2106
Clinical and Laboratory Standards Intitute (CLSI) (2008) Reference method for broth dilution testing of yeasts: approved standard-third edition M27-A3. Wayne, PA
Dubini F, Bellotti MG, Frangi A, Monti D, Saccomani L (2005) In vitro antimycotic activity and nail permeation models of a piroctone olamine (octopirox) containing transungual water soluble technology. Arzneimittelforschung 55:478–483
Dupont H, Paugam-Burtz C, Muller-Serieys C, Fierobe L, Chosidow D, Marmuse JP et al (2002) Predictive factors of mortality due to polymicrobial peritonitis with Candida isolation in peritoneal fluid in critically ill patients. Arch Surg 137:1341–1346
Kim Y, Alpmann P, Blaum-Feder S, Krämer S, Endo T, Lu D, Carson D, Schmidt-Wolf IGH (2011) Increased in vivo efficacy of lenalidomide by addition of piroctone olamine. In vivo 25:99–104
Leal AFG, Leite MC, Medeiros CSQ, Cavalcanti IMF, Wanderley AG, Magalhães NSS, Neves RP (2015) Antifungal activity of liposomal itraconazole formulation in experimental Aspergillus flavus keratitis with endophthalmitis. Mycopathologia 179:225–229
Medeiros CS, Pontes-Filho NT, Camara CA, Lima-Filho JV, Oliveira PC, Lemos SA, Leal AFG, Brandão JOC, Neves RP (2010) Antifungal activity of the naphthoquinone beta lapachone against disseminated infection whith Cryptococcus neoformans var. neoformans in dexamethasone imunossuppressed Swiss mice. Braz J Med Biol Res 43:345–349
Quintero CHG (2010) Resistencia de levaduras del género Candida al fluconazol. Infectio 14:172–180
Rebolledo M, Sarria J (2013) Intra-abdominal fungal infections. Curr Opin Infect Dis 26(5):441–446
Sherf AF (1943) A method for maintaining Phytomonas sebedonica in culture for long periods without transfer. Phytopathology 33:330–332
Sigle HC, Schafer-Korting M, Korting HC, Hube B, Niewerth M (2006) In vitro investigations on the mode of action of the hydroxypyridone antimycotics rilopirox and piroctone on Candida albicans. Mycoses 49:159–168
Tissot F, Lamoth F, Hauser PM, Orasch C, Fluckiger U, Siegemund M et al (2013) β-Glucan antigenemia anticipates diagnosis of blood culture-negative intraabdominal candidiasis. Am J Respir Crit Med 188(9):1100–1109
Yu L, Ling G, Deng X, Jin J, Jin Q, Guo N (2011) In vitro Interaction between fluconazole and triclosan against clinical isolates of fluconazole-resistant Candida albicans determined by different methods. Antimicrob Agents Chemother 55:3609–3612