Antibiotic resistant microencapsulated probiotics synergistically preserved orange juice
Tóm tắt
In contemporary medicine, the utilization of various dosage forms of probiotics is increasing both for the treatment of human and animal diseases in Bangladesh. A number of imported pharmaceutical probiotic preparations are available in the local markets at present without justification the scientific information of viability. This study was, therefore, designed to explore the viability of commercial probiotics as well as recommend the consumers for the better products in term of viability. Since probiotics preserved foods have expanded in acceptance, popularity and compliance, the goal of this research was also to investigate the combination effects of lactic acid bacteria (LAB) on development of functional foods like orange juice (OJ). Here, we screened five commercially available pharmaceutical probiotic products for rejuvenation and identification of LAB and associated resistance against different classes of antibiotics. Finally, the isolated LAB were microencapsulated and compared with free form of bacteria for biopreservation of OJ. We observed an inconsistency between the feasible live LAB counts and the declaration of the manufacturing companies. The recovered viable bacteria of pharmaceutical probiotic preparations ranged between (6.2 -7.3) × 1010 at 37 °C and (5.33-7.1) × 1010 at 25 °C, the claimed (9-10) × 1010 colony forming unit (CFU)/g. The encapsulated Lactobacillus acidophilus (LAB 1), L. bulgaricus (LAB 2), Lactococcus lactis (LAB 3) and Bifidobacterium bifidum (LAB 4) in OJ was resistant to drop out their viability as quickly as the free-form probiotic bacteria and >106 CFU/mL were still appeared after 6 wks of storage. Unencapsulated probiotics was found to have a significant reduction in viability in OJ at both 37 °C and 4 °C temperatures. However, the microencapsulation process significantly reduced the loss of viability of four probiotic bacteria as well as the control of acidification of OJ at 4 °C. The loss of potency and spoiled food associated with pathogenic microbial growth are serious problems in tropical countries including Bangladesh. The biopreserved OJ will become an important functional food due to its expansion of shelf-time, market reputation, profits and innate tastes. This report has an indication that the combination of these four LAB may become good candidate for the development of an OJ with functional characteristics.
Tài liệu tham khảo
Angiolillo L, Conte A, Zambrini AV, Del Nobile MA. Biopreservation of Fior di Latte cheese. J Dairy Sci. 2014;97:5345–55. doi:10.3168/jds.2014-8022.
El Bassi L, Hassouna M, Shinzato N, Matsui T. Biopreservation of refrigerated and vacuum-packed Dicentrarchus labrax by lactic acid bacteria. J Food Sci. 2009;74:M335–339. doi:10.1111/j.1750-3841.2009.01246.x.
Ruiz FO, Gerbaldo G, Garcia MJ, Giordano W, Pascual L, Barberis IL. Synergistic effect between two bacteriocin-like inhibitory substances produced by Lactobacilli Strains with inhibitory activity for Streptococcus agalactiae. Curr Microbiol. 2012;64:349–56. doi:10.1007/s00284-011-0077-0.
Badel S, Bernardi T, Michaud P. New perspectives for Lactobacilli exopolysaccharides. Biotechnol Adv. 2011;29:54–66. doi:10.1016/j.biotechadv.2010.08.011.
Songisepp E, Hutt P, Ratsep M, Shkut E, Koljalg S, Truusalu K, et al. Safety of a probiotic cheese containing Lactobacillus plantarum Tensia according to a variety of health indices in different age groups. J Dairy Sci. 2012;95:5495–509. doi:10.3168/jds.2011-4756.
Stykova E, Nemcova R, Valocky I, Novotny F, Guba P. Adherence of bacteria to mucus collected from different parts of the reproductive tract of heifers and cows. Can J Microbiol. 2013;59:720–5. doi:10.1139/cjm-2013-0542.
Wells JM, Mercenier A. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat Rev Microbiol. 2008;6:349–62. doi:10.1038/nrmicro1840.
Granato D, Branco GF, Nazzaro F, Cruz AG, Faria JAF. Functional foods and nondairy probiotic food development: Trends, concepts, and products. Compr Rev Food Sci Food Safety. 2010;9:292–302. doi:10.1111/j.1541-4337.2010.00110.x.
Jankovic I, Sybesma W, Phothirath P, Ananta E, Mercenier A. Application of probiotics in food products-challenges and new approaches. Curr Opin Biotechnol. 2010;21:175–81. doi:10.1016/j.copbio.2010.03.009.
Deegan LH, Cotter PD, Hill C, Ross P. Bacteriocins: Biological tools for bio-preservation and shelf-life extension. Intl Dairy J. 2006;16:1058–71. doi:10.1016/j.idairyj.2005.10.026.
Yoon KY, Woodams EE, Hang YD. Probiotication of tomato juice by lactic acid bacteria. J Microbiol. 2004;42:315–8.
Ding WK, Shah NP. Acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria. J Food Sci. 2007;72:M446–50. doi:10.1111/j.1750-3841.2007.00565.x.
Sohail A, Turner MS, Prabawati EK, Coombes AG, Bhandari B. Evaluation of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM encapsulated using a novel impinging aerosol method in fruit food products. Intl J Food Microbiol. 2012;157:162–6. doi:10.1016/j.ijfoodmicro.2012.04.025.
Tanvir EM, Afroz R, Karim N, Mottalib MA, Hossain MI, Islam M, et al. Antioxidant and antibacterial activities of methanolic extract of Bau kul (Ziziphus Mauritiana), an improved variety fruit from Bangladesh. J Food Biochem. 2015;39:139–47. doi:10.1111/jfbc.12109.
Champagne CP, Ross RP, Saarela M, Hansen KF, Charalampopoulos D. Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. Intl J Food Microbiol. 2011;149:185–93.
Hossain MS, Al-Bari MAA, Wahed MII. Biochemical characterization of probiotics available in Bangladesh. J Sci Res. 2016; 8:101-108. doi: http://dx.doi.org/10.3329/jsr.v8i1.25299.
Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, et al. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip. 2015;29:84–91. doi:10.1080/13102818.2014.987450.
Vandepitte J, Verhaegen J, Engbaek K, Rohner P, Piot P, Heuck CC. Basic laboratory procedures in clinical bacteriology. 2nd ed. Geneva: World Health Organization; 2003.
Pereira-Caro G, Oliver CM, Weerakkody R, Singh T, Conlon M, Borges G, et al. Chronic administration of a microencapsulated probiotic enhances the bioavailability of orange juice flavanones in humans. Free Radical Biol Med. 2015;84:206–14. doi:10.1016/j.freeradbiomed.2015.03.010.
Ding WK, Shah NP. Survival of free and microencapsulated probiotic bacteria in orange and apple juices. Intl Food Res J. 2008;15(2):219–32.
Shah NP, Ravula RR. Influence of water activity on fermentation, organic acids production and viability of yogurt and probiotic bacteria. Aust J Dairy Technol. 2000;55:127–31.
Zhang T, McCarthy J, Wang G, Liu Y, Guo M. Physiochemical properties, microstructure, and probiotic survivability of nonfat goats’ milk yogurt using heat-treated whey protein concentrate as fat replacer. J Food Sci. 2015;80:M788–94. doi:10.1111/1750-3841.12834.
Wong A, Ngu DYS, Dan LA, Ooi A, Lim RLH. Detection of antibiotic resistance in probiotics of dietary supplements. Nutr J. 2015;14:95. doi:10.1186/s12937-015-0084-2.
Selim AS, Haider G. Studies on the viabile bacteria of commercial probiotic products available in Bangladesh. World J Microbiol. 2014;1:010–2.
Cleveland J, Montville TJ, Nes IF, Chikindas ML. Bacteriocins: safe, natural antimicrobials for food preservation. Intl J Food Microbiol. 2001;71:1–20.
Champagne CP, Raymond Y, Tompkins TA. The determination of viable counts in probiotic cultures microencapsulated by spray-coating. Food Microbiol. 2010;24:1104–11.
Farnworth ER. The evidence to support health claims for probiotics. J Nutri. 2008;138:1250S–4S.
Hamilton-Miller JM, Shah S, Winkler JT. Public health issues arising from microbiological and labelling quality of foods and supplements containing probiotic microorganisms. Public Health Nutri. 1999;2:223–9.
de Souza Oliveira RP, Perego P, de Oliverira MN, Converti A. Effect of inulin as prebiotic and synbiotic interactions between probiotics to improve fermented milk firmness. J Food Engineer. 2011;107:36–40. doi:10.1016/j.jfoodeng.2011.06.005.
Lahtinen SJ. Probiotic viability - does it matter? Microbial Ecol Health Dis. 2012;23:18567. doi:10.3402/mehd.v23i0.18567.
Przyrembel H. Consideration of possible legislation within existing regulatory frameworks. Am J Clin Nutri. 2001;73:471S–5S.
Gillor O, Etzion A, Riley MA. The dual role of bacteriocins as anti- and probiotics. Appl Microbiol Biotechnol. 2008;81:591–606. doi:10.1007/s00253-008-1726-5.
Costelloe C, Metcalfe C, Lovering A, Mant D, Hay AD. Effect of antibiotic prescribing in primary care on antimicrobial resistance in individual patients: systematic review and meta-analysis. BMJ. 2010;340:c2096. doi:10.1136/bmj.c2096.
Goossens H, Ferech M, Vander Stichele R, Elseviers M. Outpatient antibiotic use in Europe and association with resistance: a cross-national database study. Lancet. 2005;365:579–87. doi:10.1016/S0140-6736(05)70799-6.
Devirgiliis C, Zinno P, Perozzi G. Update on antibiotic resistance in foodborne Lactobacillus and Lactococcus species. Front Microbiol. 2013;4:301. doi:10.3389/fmicb.2013.00301.
Gueimonde M, Sánchez B, de los Reyes-Gavilán CG, Margolles A. Antibiotic resistance in probiotic bacteria. Front Microbiol. 2013;4:202. doi:10.3389/fmicb.2013.00202.
Djordjevic SP, Stokes HW, Chowdhury PR. Mobile elements, zoonotic pathogens and commensal bacteria: conduits for the delivery of resistance genes into humans, production animals and soil microbiota. Front Microbiol. 2013;4:86. doi:10.3389/fmicb.2013.00086.
Rolain JM. Food and human gut as reservoirs of transferable antibiotic resistance encoding genes. Front Microbiol. 2013;4:173. doi:10.3389/fmicb.2013.00173.
Tsen JH, Lin YP, An-Erl KV. Fermentation of banana media by using k-carrageenan immobilized Lactobacillus acidophilus. Intl J Food Microbiol. 2004;91:215–20.
Ford AC, Quigley EM, Lacy BE, Lembo AJ, Saito YA, Schiller LR, et al. Efficacy of prebiotics, probiotics, and synbiotics in irritable bowel syndrome and chronic idiopathic constipation: systematic review and meta-analysis. Am J Gastroenter. 2014;109:1547–61. doi:10.1038/ajg.2014.202.
Ding WK, Shah NP. An improved method of microencapsulation of probiotic bacteria for their stability in acidic and bile conditions during storage. J Food Sci. 2009;74:M53–61. doi:10.1111/j.1750-3841.2008.01030.x.
Kailasapathy K. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. LWT - Food Sci Technol. 2006;39:1221–7. doi:10.1016/j.lwt.2005.07.013.
Saarela M, Alakomi HL, Mättö J, Ahonen AM, Tynkkynen S. Acid tolerant mutants of Bifidobacterium animalis subsp. lactis with improved stability in fruit juice. LWT Food Sci Technol. 2011;44:1012–8.
Aneja KR, Dhiman R, Aggarwal KN, Aneja A. Emerging preservation techniques for controlling spoilage and pathogenic microorganisms in fruit juices. Intl J Microbiol. 2014. http://dx.doi.org/10.1155/2014/758942.
Jabeen F, Qazi JI. Potential of bacterial chitinases and exopolysaccharides for enhancing shelf life of food commodities at varying conditions. Intl Res J Environ Sci. 2014;3:87–93.
Fuglsang CC, Johansen CC, Christgau SS, Adler-Nissen J. Antimicrobial enzymes: Applications and future potential in the food industry. Trends Food Sci Technol. 1995;6:390–6.
Pérez-Pérez C, Regalado-González C, Rodríguez-Rodríguez CA, Barbosa-Rodríguez JR, Villaseñor-Ortega F. Incorporation of antimicrobial agents in food packaging films and coatings. Adv Agri Food Biotechnol. 2006;37:193–216.