Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

AMB Express - Tập 11 Số 1 - 2021
Amir Mirzaie1, Reza Ranjbar1
1Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Tóm tắt

AbstractKlebsiella pneumoniaeis a multidrug-resistant (MDR) opportunistic pathogen that causes nosocomial infections. Virulence analysis and molecular typing as powerful approaches can provide relevant information onK. pneumoniaeinfection. In the current study, antibiotic resistance, virulence-associated genes analysis, as well as molecular typing ofK. pneumoniaestrains were investigated. Out of 505 clinical samples collected from hospitalized patients, 100K. pneumoniaestrains were isolated by standard microbiological methods and subjected to the phenotypic and genotyping analysis. The highest prevalence of resistance was observed against ciprofloxacin (75%), trimethoprim–sulfamethoxazole (73%) and nitrofurantoin (68%). Virulence associated genes includingentB,traT,ybts, magA, iucC, htrAandrmpAwere found in 80%, 62%, 75%, 5%, 30%, 72% and 48%, of the isolates, respectively. The prevalence of biofilm-associated genes includingmrkA, fimH, andmrkDwere equally 88% for all tested isolates. Moreover, the efflux pump genes includingAcrAB, TolCandmdtKwere observed in 41 (41%), 33 (33%) and 26 (26%) of the strains respectively. A significant statistical association was observed between MDR strains and high expression of efflux pump and biofilm genes. TheK. pneumoniaestrains were differentiated into 11 different genetic patterns using the repetitive element sequence-based PCR (rep-PCR) technique. High prevalence of resistance, presence of various virulence factors, high level of efflux pump, and biofilm gene expression in diverse clones ofK. pneumoniaestrains pose an important health issue in clinical settings.

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