Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae)
Tóm tắt
The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum. Biological testing revealed that the plant extracts possess antisickling effects. The combination of spectroscopic techniques: 1D-NMR, 2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O. gratissimum (Silva et al. in Molecules 13:2482–2487, 2008; Kedar et al. J Food Drug Anal 20:865–871, 2012). This study is the first report of the antisickling activity of ursolic acid isolated from O. gratissimum. The pharmaceutical relevance of findings from this study derives from the possibility of integrating O. gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo. The identification of the active principle could enhance the standardization of antisickling recipe.
Tài liệu tham khảo
M.G.V. Silva, I.G.P. Vieira, F.N.P. Mendes, I.L. Albuquerque, R.N. dos Santos, F.O. Silva, S.M. Morais, Molecules 13, 2482–2487 (2008)
K.R. Kedar, K.S. Rajesh, P.B. Durga, K.M. Sagar, J. Food Drug Anal. 20(4), 865–871 (2012)
K.N. Ngbolua, V. Mudogo, P.T. Mpiana, M.J. Malekani, R. Herintsoa, S. Urverg Ratsimamanga, L. Takoy, H. Rakotoarimana, D.S.T. Tshibangu, Ethnopharmacologia 50, 19–24 (2013)
P.T. Mpiana, B.L. Kimbadi, A.M. Ombeni, K.N. Ngbolua, D.S.T. Tshibangu, D.D. Tshilanda, N.M. Misengabu, C.L. Muanyishay, S.K. Muyisa, Open J. Blood Dis. 3, 43–48 (2013)
D.L. Nelson, M.M. Cox, Lehninger Principles of Biochemistry, 5th edn. (WH Freeman and Company, New York, 2008), pp. 75–189
P.T. Mpiana, L.K. Makelele, R.W. Oleko, M.T. Bokota, D.S.T. Tshibangu, K.N. Ngbolua, S.M. Nsimba, M.B. Mbala, E.K. Atibu, Aust. J. Med Herbal 22(4), 132–137 (2010)
P.T. Mpiana, M.T. Bokota, J.P. Mbula, K.N. Ngbolua, D.S.T. Tshibangu, E.K. Atibu, J.T.K. Kwembe, M.K. Kakule, in Anthocyanins: Structure, Biosynthesis and Health Benefits, ed. by N. Motohashi (Nova Science Publishers, New York, 2012), pp. 111–124. (Chap. 5)
P.T. Mpiana, K.N. Ngbolua, V. Mudogo, D.S.T. Tshibangu, E.K. Atibu, in Progress in Traditional and Folk Herbal Medicine, vol. I, ed. by V.K. Gupta (Daya Publishing House, New Delhi, 2012), pp. 1–11
P.T. Mpiana, F.M. Misakabu, J.M. Kitadi, K.N. Ngbolua, D.S.T. Tshibangu, B.K. Lombe, P.V. Tsalu, E.K. Atibu, B.Z. Gbolo, C.L. Muanishay, in Anthocyanins: Occurrence, Structure, Biosynthesis and Health Benefits Based on their Evidences of Phytochemicals in Vegetables and Fruits, vol. 2, ed. by N. Motohashi (Nova Science Publishers, New York, 2014), pp. 97–113
D.P. Clark, N.J. Pazdernik, Molecular Biology, 2nd edn. (Academic Press, Waltham, MA, 2013), pp. 417–457
World Health Geneva: World Health Organization; 2011. [Online] Available from: http://www.who.int/mediacentre/factsheets/fs308/en/. Accessed 20 June 2013
P.T. Mpiana, D.S.T. Tshibangu, O.M. Shetonde, K.N. Ngbolua, Phytomedicine 14, 192–195 (2007)
P.T. Mpiana, V. Mudogo, D.S.T. Tshibangu, O.M. Shetonde, K.N. Ngbolua, P.K. Mangwala, B.K. Mavakala, J. Med. Sci. 7(7), 1182–1186 (2007)
P.T. Mpiana, E.N. Dianzenza, K.N. Ngbolua, V. Mudogo, D.S.T. Tshibangu, B.M. Mbala, O.M. Shetonde, E.K. Atibu, M.K. Kakule, M.T. Bokota, Int. J. Biol. Chem. Sci. 6(5), 2241–2251 (2012)
P.T. Mpiana, K.N. Ngbolua, V. Mudogo, D.S.T. Tshibangu, E.K. Atibu, D.D. Tshilanda, N.M. Misengabu, J. Med. Sci. 11(3), 129–137 (2011)
J. Makani, S.F. Ofori-Acquah, O. Nnodu, A. Wonkam, K. Ohene-Frempong, Sci. World J. (2013)
H. Ibrahim, F.S. Sani, B.H. Danladi, A.A. Ahmadu, Pak. J. Biol. Sci. 10(5), 788–791 (2007)
J. Okpuzor, O. Adebesin, H. Ogbunugafor, I. Amadi, Int. J. Biomed. Health Sci. 4(2), 47–55 (2008)
M. Sahu, V. Singh, S. Yadah, K.K. Harris, J. Phytol. 4(3), 24–29 (2012)
K.M. Meselhy, L.N. Hammad, N. Farag Life Sci. J. 9(3), 830–841 (2012)
P.T. Mpiana, V. Mudogo, D.S.T. Tshibangu, E.K. Kitwa, A.B. Kanangila, J.B.S. Lumbu, K.N. Ngbolua, E.K. Atibu, M.K. Kakule, J. Ethnopharmacol. 120, 413–418 (2008)
P.T. Mpiana, V. Mudogo, D.S.T. Tshibangu, K.N. Ngbolua, D.D. Tshilanda, E.K. Atibu, in Chemistry and Medicinal Value, ed. by V.K. Singh, J.N. Govil (Studium Press LLC, Houston, 2009), pp. 83–90
P.T. Mpiana, V. Mudogo, K.N. Ngbolua, D.S.T. Tshibangu, E.K. Atibu, E.K. Kitwa, A.B. Kanangila, in Chemistry and Medicinal Value, ed. by V.K. Singh, J.N. Govil (Studium Press LLC, Houston, 2009), pp. 75–82
P.T. Mpiana, V. Mudogo, D.S.T. Tshibangu, K.N. Ngbolua, P.K. Manguala, E.K. Atibu, M.K. Kakule, L.K. Makelele, M.T. Bokota, Comp. Biol. Nat. Pro. 3, 287–295 (2010)
P.T. Mpiana, V. Mudogo, D.S.T Tshibangu, O.M. Shetonde, K.N. Ngbolua, M.B. Mbala, Antisickling activity of some Congolese plants. Paper presented at the 12th symposium of the Natural Product Research Network for Eastern and Central Africa (NAPRECA), Makerere University, Kampala, Uganda, Jul 22–26 July 2007, p. 45
K.N. Ngbolua, P.T. Mpiana, D.S.T. Tshibangu, P.P. Mazasa, B.Z. Gbolo, E.K. Atibu, J.N. Kadima, F.M. Kasali, Eur. J. Med. Plants 4(10), 1251–1267 (2014)
D.D. Tshilanda, P.T. Mpiana, D.N.V. Onyamboko, B.M. Mbala, K.N. Ngbolua, D.S.T. Tshibangu, M.K. Bokolo, K.M. Taba, T.K. Kasonga, Asian Pac. J. Trop. Biomed. 4(1), 930–935 (2014)
D.S.T Tshibangu, F.O. Shode, N. Koorbanally, V. Mudogo, P.T. Mpiana, K.N. Ngbolua, Antisickling triterpenoids from Callistemon viminalis, Meulaleuca bracteata var. Revolution Gold Syzygium guineense and Syzygium cordatum. Paper presented at the 14th NAPRECA Symposium and AAMPS Ethno-veterinary Medicine Symposium, Internatiuonal Centre for Insect Physiology and Ecology (ICIPE), Kasarani, Nairobi, Kenya, 8–12 August 2011, pp. 296–300 (YS 27)
S. Sang, K. Lapsley, R.T. Rosen, C.T. Ho, J. Agric. Food Chem. 50, 607–609 (2002)
S.B. Mahato, A.P. Kundu, Phytochemistry 37(6), 1517–1575 (1994)
S.C.B. Gnoatto, A. Dassonville-Klimpt, S. Da Nascimento, P. Galera, K. Boumediene, G. Gosman, P. Sonnet, S. Moslemi, Eur. J. Med. Chem. 43, 1865–1877 (2008)
F.M. Moghaddam, M.M. Farimani, S. Salahvarzi, G. Amin, Evid. Based Alternat. Med. 4(1), 95–98 (2007)
B.K. Neoh, U.U. Rahayu, H.L. Nordin, Y.C. Tai, Y.L. Tu, R. Mawardi, A.S. Mohd, Malays. J. Anal. Sci. 14(1), 6–11 (2010)
B. Moulisha, G.A. Kumar, H.P. Kanti, Trop. J. Pharm. Res. 9(2), 135–140 (2010)
L. Ogunkoya, Phytochemistry 20, 121–126 (1981)
A.R. Gohari, S. Saeidnia, A.R. Shahverdi, N. Yassa, M. Malmir, K. Mollazade, A.R. Naghinejad, Eur. Asian J. BioSci. 3, 64–68 (2009)
I.T. Babalola, F.O. Shode, J. Pharmacogn. Phytochem. 2(2), 214–222 (2013)
J. Wang, Z. Jiang, L. Xiang, Y. Li, O. Minrui, X. Yang, J. Shao, L. Yusheng, L. Lin, J. Chen, Y. Dai, L. Jia, Sci. Rep. 4, 5006 (2014)
K.N. Ngbolua, T.T. Bishola, P.T. Mpiana, V. Mudogo, D.S.T. Tshibangu, K.N. Ngombe, E.G. Ekutsu, D.D. Tshilanda, B.Z. Gbolo, D.T. Mwanangombo, P.R. Fatiany, R. Baholy, Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae). J. Adv. Bot. Zool. (2014). doi:10.15297/JABZ.V1I2.04
P.T. Mpiana, M.T. Bokota, M.B.L. Ndjele, V. Mudogo, D.S.T. Tshibangu, K.N. Ngbolua, E.K. Atibu, J.T.K. Kwembe, L.K. Makelele, Int. J. Biol. Chem. Sci. 4(6), 1953–1961 (2010)
R.L. Wixon, C.W. Gehrke (eds.), Chromatography: A Science of Discovery (John Wiley and Sons, Inc, Hoboken, 2011), pp. 61–100
D.C. Dash, Analytical Chemistry (PHI Learning Pvt. Ltd, New Delhi, 2011), pp. 213–262