Anti-inflammatory effects of Athyrium yokoscense extract via inhibition of the Erk1/2 and NF-κB pathways in bisphenol A-stimulated A549 cells

Toxicological Research - Tập 39 - Trang 135-146 - 2022
Jung-Kyu Lee1, Won Seok Choi1, Jin Yong Song1, Oh Seong Kwon1, Yeon Jin Lee1, Jong Seok Lee2, Sarah Lee2, Se Rin Choi3, Choong Hwan Lee3, Ji-Yun Lee1
1College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
2National Institute of Biological Resources, Incheon, Republic of Korea
3Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea

Tóm tắt

Bisphenol A is an environmental endocrine disruptor that has similar functions to estrogen in humans. However, few studies have investigated pulmonary inflammation induced by BPA, and the effect of Athyrium yokoscense extract on this inflammatory response is unknown. In this study, we investigated this effect in A549 human alveolar epithelial cells. BPA at concentrations higher than 100 µM were cytotoxic to A549 cells at 24 and 48 h after treatment; however, AYE (100 µg/mL) had a protective effect against BPA-induced cytotoxicity. AYE also inhibited the generation of intracellular reactive oxygen species, expressions of cyclooxygenase-2 and extracellular signal-regulated kinase1/2 proteins, activities of phospholipase A2, COX-2, nuclear factor kappa-light-chain-enhancer of activated B cells, and proinflammatory mediators including prostaglandin E2, tumor necrosis factor-α, and interleukin-6 induced by BPA in A549 cells. This study demonstrated that BPA, which induces chronic lung disease, causes oxidative stress and inflammatory response in lung epithelial cell line, and found that AYE reduces BPA-induced oxidative stress and inflammatory response by down-regulating the Erk1/2 and NF-κB pathways.

Tài liệu tham khảo

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