Anti-inflammatory and antioxidant properties of Alternanthera brasiliana improve cutaneous wound healing in rats

Inflammopharmacology - Tập 29 - Trang 1443-1458 - 2021
Rogério Marchete1, Sarah Oliveira1, Leonardo Bagne1, Jennyffer Ione de Souza Silva1, Ana Paula Valverde1, Andrea Aparecida de Aro1, Mariana Moreira Figueira2, Marcio Fronza2, Thainá Mikaela Bressam1, Vivian Fernandes Furletti de Goes3, Fernanda Oliveira de Gaspari de Gaspi1,4, Gláucia Maria Tech dos Santos1, Thiago Antônio Moretti Andrade1
1Graduate Program in Biomedical Sciences, University Center of Herminio Ometto Foundation–FHO, Araras, Brazil
2Graduate Program of Pharmaceutical Sciences, Laboratory of Natural Products, University of Vila Velha–UVV, Vila Velha, Brazil
3Graduate Program in Odontology, University Center of Herminio Ometto Foundation–FHO, Araras, Brazil
4Sao Leopoldo Mandic Faculty, Araras, Brazil

Tóm tắt

Alternanthera brasiliana (L.) Kuntze is recognized for its healing properties; however, its therapeutic effects remain unclear. Therefore, our study aimed to elucidate the wound healing activities of A. brasiliana using in vitro and in vivo assays. In vitro assays were used to evaluate the antibacterial, anti-inflammatory, and antioxidant effects of A. brasiliana extract. For the in vivo study, two dorsal excisions were established in Wistar rats using a punch (1.5 cm in diameter), which were topically treated daily with 2% carbopol gel (Ctrl group) or 20% hydroalcoholic plant extract with 2% carbopol gel (A. brasiliana–Ab group). After the 2nd, 7th, 14th, and 21st days, inflammation, oxidative damage, antioxidants, angiogenesis, tissue formation, and re-epithelialization were evaluated. In vitro, Ab reduced nitric oxide, anion superoxide, and pro-inflammatory cytokine production. In vivo, Ab presented lower levels of inflammatory infiltrate, although increased levels of IL-1β and TGF-β1 were observed. The plant extract controlled oxidative damage by antioxidants, which favored angiogenesis, collagenesis, and wound re-epithelialization. Thus, the topical application of the hydroalcoholic extract of 20% A. brasiliana was distinguished by its important anti-inflammatory and antioxidant activities both in vivo and in vitro. The plant extract also stimulated angiogenesis and tissue formation, accelerating total re-epithelization, which is promising for wound healing.

Tài liệu tham khảo

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