Analysis of transfer of microinjected zygotes in production of transgenic mice
Tóm tắt
The data on transfer of mouse eggs microinjected with DNA during production of transgenic mice were analyzed. The transfer of mouse eggs into both oviducts did not lead to a reliably higher birth rate. It did not affect the frequency of recipients’ pregnancy and, although somewhat increased the frequency of multiple birth, led, finally, to unjustified loss of the major part of viable DNA-injected eggs. We recommend transferring no less than 15 microinjected eggs only in one oviduct of each recipient. The transfer into another oviduct is acceptable if the transfer into the first oviduct failed or its outcome is doubtful.
Tài liệu tham khảo
Brinster, R.L., Chen, H.Y., Trumbauer, M.E., et al., Factors Affecting the Efficiency of Introducing Foreign DNA Into Mice by Microinjecting Eggs, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 4438–4442.
Goldman, I.L., Kadulin, S.G., and Razin, S.V., Transgenic Animals in Medicine: Integration and Expression of Foreign Genes, Theoretical and Applied Aspects, Med. Sci. Monit., 2004, vol. 10, no. 11, pp. RA274–285.
Murphy, D. and Hanson, J., Production of Transgenic Mice by Microinjecting Cloned DNA into Fertilized Eggs, Novoe v klonirovanii DNK. Metody (News in Cloning DNA. Methods), Glover, D., Ed., Moscow: Mir, 1989, Chapter 10.
Posobie k bol’shomu praktikumu po embriologii (A Manual on Practicum of Embryology), Beloussov, L.V., et al., Eds., Moscow: Mosk. Gos. Univ., 1990.
Wall, R.J. and Siedel, G.E., Jr. Transgenic Farm Animals—a Critical Analysis, Theriogenology, 1992, vol. 38, pp. 337–357
Wood, M., Whittingham, D., and Rol, U., Low Temperature Conservation of Mouse Eggs and Embryos, Biologiya razvitiya mlekopitayushchikh. Metody (Developmental Biology of Mammals. Methods), Mank, M., Ed., Moscow: Mir, 1990.