Analysis of phytochemical variations in dioecious Tinospora cordifolia stems using HPLC/QTOF MS/MS and UPLC/QqQLIT‐MS/MS

Phytochemical Analysis - Tập 27 Số 2 - Trang 92-99 - 2016
Vikas Bajpai1,2, Awantika Singh1,2, Preeti Chandra1,2, M.P.S. Negi3, Nikhil Kumar4, Brijesh Kumar1,2
1Academy of Scientific and Innovative Research, New Delhi 110001, India
2Sophisticated Analytical Instrument Facility Division, CSIR-Central Drug Research Institute, Lucknow 226031, India
3Biometry and Statistics Division, CSIR-Central Drug Research Institute, Lucknow, 226031 India
4Betelvine Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India

Tóm tắt

AbstractIntroductionThe stem of dioecious Tinospora cordifolia (Menispermaceae) is a commonly used traditional Ayurvedic medicine in India having several therapeutic properties.ObjectiveTo develop and validate LC‐MS methods for the identification and simultaneous quantitation of various secondary metabolites and to study metabolomic variations in the stem of male and female plants.MethodsEthanolic extract of stems were analysed by HPLC/ESI‐QTOF‐MS/MS for rapid screening of bioactive phytochemicals. High resolution MS and MS/MS in positive ESI mode were used for structural investigation of secondary metabolites. An UPLC/ESI‐QqQLIT‐MS/MS method in MRM mode was developed and validated for the simultaneous quantitation of five bioactive alkaloids.ResultsIdentification and characterisation of 36 metabolites including alkaloids, sesquiterpenes and phytoecdysteroids were performed using LC‐MS and MS/MS techniques. The bioactive alkaloids such as jatrorrhizine, magnoflorine, isocorydine, palmatine and tetrahydropalmatine were successfully quantified in male and female plants. The mean abundances of magnoflorine jatrorrhizine, and oblongine were significantly (P < 0.05) higher in male plants while mean abundances of tetrahydropalmatine, norcoclaurine, and reticuline were significantly (P < 0.05) higher in female plants.ConclusionsPhytochemicals in the stem of male and female Tinospora cordifolia showed significant qualitative and quantitative variations. LC‐MS and MS/MS methods can be used to differentiate between male and female plants based on their chemical profiles and quantities of the marker bioactive alkaloids. This chemical composition difference was also evident during vegetative stage when there were no male and female flowers. Copyright © 2015 John Wiley & Sons, Ltd.

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Phytochemical Analysis

Tập 27 Số 2

92-99

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