Phân tích biểu hiện miRNA liên quan đến gen Lr46 chịu trách nhiệm kháng APR ở lúa mì (Triticum aestivum L.)

Journal of Applied Genetics - 2020
Agnieszka Tomkowiak1, Tomasz Jędrzejewski1, Julia Spychała1, Jakub Kuczyński2, Michał Kwiatek1, Agata Tyczewska2, Roksana Skowrońska1, Tomasz Twardowski2
1Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Dojazd str. 11, 60-632, Poznań, Poland
2Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego str. 12/14, 61-704, Poznań, Poland

Tóm tắt

Tóm tắtLr46/Yr29/Pm39(Lr46) là một gen chịu trách nhiệm về khả năng kháng nấm sắt chậm ở lúa mì. Mục tiêu của nghiên cứu là phân tích biểu hiện miRNA ở các giống lúa mì thông dụng được chọn mang các gen kháng, trong đó có Lr46 (HN Rod, Pavon‘S’, Myna‘S’, Frontana‘S’, và Sparrow’S’) để đáp ứng lại sự nhiễm nấm đốm lá do Puccinia triticinaErikss gây ra. Trong các giống Pavon ‘S’, Myna ‘S’, Frontana ‘S’, và Sparrow ‘S’, một sản phẩm có chiều dài 242 bp đã được xác định, sản phẩm này đặc hiệu cho dấu hiệu Xwmc44 liên kết với gen kháng nấm sắt Lr46. Ở bước tiếp theo, sự khác biệt trong biểu hiện microRNA (miR5085 và miR164) có liên quan đến gen Lr46 đã được kiểm tra, gen này chịu trách nhiệm cho khả năng kháng khác nhau của các giống lúa mì đã chọn đối với bệnh đốm lá, bằng cách sử dụng PCR nhũ tương (ddPCR). Trong thí nghiệm, căng thẳng sinh học đã được kích thích ở các cây trưởng thành bằng cách gây nhiễm chúng bằng bào tử nấm trong điều kiện kiểm soát trong buồng phát triển. Để phân tích, vật liệu thực vật đã được thu thập trước khi nhiễm và 6, 12, 24, và 48 giờ sau khi nhiễm. Các thí nghiệm cũng cho thấy rằng việc nhiễm cây bằng Puccinia triticina đã dẫn đến sự gia tăng biểu hiện miR164 ở các giống mang gen Lr46. Biểu hiện của miR164 vẫn ổn định trong giống đối chứng (HN ROD) không có gen này. Điều này đã chứng minh rằng miR164 có thể tham gia vào các cơ chế kháng nấm đốm lá.

Từ khóa


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Journal of Applied Genetics

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