Analysis of glutathione endpoints for measuring copper stress in Chlamydomonas reinhardth
Tóm tắt
Glutathione (GSH) is the most abundant nonprotein thiol in eukaryotic cells and it protects cells by functioning as an antioxidant and a metal‐binding ligand. Because glutathione readily undergoes oxidation‐reduction reactions to combat oxidative stress, intracellular ratios of the reduced (GSH) to the oxidized (GSSG) forms of glutathione may serve as an important biomarker of exposure and effect of trace metals in eukaryotic cells. We compared sensitivity of glutathione ratios in the freshwater alga
Từ khóa
Tài liệu tham khảo
Morel FMM, 1975, Description of the algal growth media “Aquil” and “Fraquil”
Guillard RRL, 1973, Handbook of Phycological Methods. Culture Methods and Growth Measurements, 69
Arar EJ, 1997, Method 445.0. In vitro determination of chlorophyll a and pheophytin a in marine and freshwater algae by fluorescence