Analysis of aqueous humour proteins in patients with retinoblastoma

Clinical and Experimental Ophthalmology - Tập 40 Số 1 - 2012
Theodora Hadjistilianou1, Stefania Giglioni2, L. Micheli2, Davide Vannoni2, Elena Brogi2, Gabriele Cevenini3, Alessio Cortelazzo2, Sonia De Francesco1, Felice Menicacci4, Roberto Leoncini2
1Retinoblastoma Referral Center, Ophthalmology Unit
2Department of Internal Medicine, Endocrine‐Metabolic Sciences and Biochemistry
3Department of Surgery and Bioengineering
4Ophthalmology Unit University of Siena Siena Italy

Tóm tắt

AbstractBackground:  To investigate aqueous humour protein composition from retinoblastoma patients.Design:  Prospective, hospital‐based study.Participants:  Eighteen retinoblastoma patients (Reese–Ellsworth stage V or ABC classification group E RB) undergoing ocular enucleation, and 10 normal subjects undergoing cataract surgery. Five of 18 patients presented with associated secondary glaucoma whereas 13 had no secondary glaucoma; 5 of 13 patients with no secondary glaucoma received chemotherapeutical treatment with melphalan.Methods:  Aqueous humour samples were collected by limbal paracentesis of the anterior chamber after ocular enucleation in patients and after the stab peripheral corneal incision in controls. Total protein concentration according to Bradford method and sodium dodecyl sulphate‐polyacrylamide gel electrophoresis of the samples were performed.Main Outcome Measure:  Aqueous humour protein concentration.Results:  Aqueous humour protein concentration was significantly higher in retinoblastoma patients than controls (P < 0.01); patients with secondary glaucoma presented the highest values (P < 0.05 vs. controls); patients treated with melphalan presented a significant decrease (P < 0.01) versus non‐treated; controls did not significantly differ from treated patients. Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis pattern in retinoblastoma patients who did not receive any treatment was very different either from treated or from controls.Conclusion:  This study represents a preliminary step towards a more accurate two dimensional electrophoresis (2DE) pattern, which will be combined with mass spectrometry analysis to clarify the potential role of specific proteins in tumour development and progression; although these results suggest that aqueous humour protein pattern in retinoblastoma is characteristic, several aspects of the study are still under investigation.

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Tài liệu tham khảo

10.1097/ICU.0b013e328312975b

10.1136/bjo.2009.168062

10.1186/1750-1172-1-31

Balmer A, 2007, Differential diagnosis of leukocoria and strabismus, first presenting signs of retinoblastoma, Clin Ophthalmol, 1, 431

10.1073/pnas.68.4.820

10.1073/pnas.72.12.5116

10.1002/(SICI)1098-1004(199910)14:4<283::AID-HUMU2>3.0.CO;2-J

10.1002/ijc.22944

Mastrangelo D, 2008, The retinoblastoma paradigm revisited, Med Sci Monit, 14, RA231

10.1007/s10038-005-0348-3

10.1016/j.acthis.2007.07.002

10.1111/j.1349-7006.2008.01070.x

10.1038/sj.emboj.7601515

10.1016/j.humpath.2007.12.008

10.1038/sj.bjc.6600532

10.1074/jbc.M806133200

10.1038/onc.2008.200

10.1038/onc.2008.15

10.1002/ijc.21945

Ozakyol A, 2006, Fish detected p53 deletion and N‐MYC amplification in colorectal cancer, Hepatogastroenterology, 53, 192

10.1016/S0014-4835(77)80015-8

10.1046/j.1440-1681.2000.03208.x

10.1111/j.1444-0938.2002.tb02384.x

10.1159/000306003

10.1023/B:INTE.0000018524.48581.79

10.1016/j.jprot.2010.10.002

Richardson MR, 2009, Proteomic analysis of human aqueous humor using multidimensional protein identification technology, Mol Vis, 15, 2740

10.1016/j.ophtha.2007.12.014

10.1016/0003-2697(76)90527-3

10.1111/j.1462-5822.2007.00901.x

Duan X, 2008, Proteomic analysis of aqueous humor from patients with myopia, Mol Vis, 14, 370

10.1111/j.1600-0420.2005.00381.x

10.1097/00008390-200312000-00013

10.1007/s12575-009-9019-7

10.1167/iovs.09-3913

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Clinical and Experimental Ophthalmology

Tập 40 Số 1

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