Analysis of Rhizoma Polygoni Cuspidati by HPLC and HPLC‐ESI/MS

Phytochemical Analysis - Tập 18 Số 5 - Trang 387-392 - 2007
Tao Yi1, Hao Zhang1, Zongwei Cai2
1West China School of Pharmacy, Sichuan University, No. 17, Section 3, Ren-Min-Nan-Lu Road, Chengdu, Sichuan 610041, People's Republic of China
2Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, People's Republic of China

Tóm tắt

AbstractAn HPLC method with photodiode array detection (PAD) and ESI/MS detection was developed for the qualitative and quantitative analysis of the major chemical constituents of the dried rhizome of Polygonum cuspidatum Sieb. et Zucc. (Rhizoma Polygoni Cuspidati; Chinese name Hu‐Zhang). Based on the chromatographic separation on an Altima C18 column using 0.5% aqueous acetic acid and acetonitrile as the mobile phase, nine compounds, including stilbenes, stilbene glucosides, anthraquinones and anthraquinone glucosides, were identified by online ESI/MS analysis and seven were quantified by HPLC‐PAD. A full validation of the method including sensitivity, linearity, repeatability and recovery was conducted. Linear calibration was achieved over the concentration range 1–200 mg/L with R2 > 0.999, whilst the limits of detection ranged from 0.51 to 1.57 ng. Repeatability was evaluated by intra‐ and inter‐day assays and the RSD value was within 1.79%. Recoveries of the quantified compounds were within the range 96.0–100.1% with RSD values of less than 2.2%. Five samples of Rhizoma Polygoni Cuspidati from different regions were analysed using the developed method. The major constituents piceid, resveratrol, emodin‐8‐O‐β‐d‐glucoside and emodin were selected to provide an index for the quality assessment of the herbal drug. Copyright © 2007 John Wiley & Sons, Ltd.

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Thông tin xuất bản

Phytochemical Analysis

Tập 18 Số 5

387-392

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