Analysis of Eleven Phenolic Compounds Including Novel p‐Coumaroyl Derivatives in Lettuce (Lactuca sativa L.) by Ultra‐high‐performance Liquid Chromatography with Photodiode Array and Mass Spectrometry Detection

Phytochemical Analysis - Tập 22 Số 6 - Trang 555-563 - 2011
Albert Ibarz Ribas1, Marta Gratacós-Cubarsí1, Carmen Sárraga1, José‐Antonio García‐Regueiro1, Massimo Castellari1
1Institute for Food and Agriculture Research (IRTA) Food Technology Finca Camps i Armet s/n, Monells 17121 Girona Catalonia Spain

Tóm tắt

ABSTRACTIntroductionLettuce is a widely consumed vegetable and a good source of phenolic compounds. Several factors (genetic, agronomical and environmental) can influence the lettuce composition; their effects are not completely defined and more studies are needed on this topic.ObjectiveTo develop an improved ultra‐high‐performance liquid chromatography (UHPLC) method to quantify the main target intact phenolic compounds in lettuce.MethodologyUHPLC identification of the compounds was supported by PAD spectra and MSn analyses. Quantification was carried out by PAD, by creating matrix‐matched calibration curves at the specific wavelength for each compound.ResultsSample pretreatment was simplified, with neither purification nor hydrolysis steps. Chromatographic conditions were chosen to minimise matrix interferences and to give a suitable separation of the major phenolic compounds within 27 min. The method allowed the quantification of 11 intact phenolic compounds in Romaine lettuces, including phenolic acids (caffeoyl and p‐coumaroyl esters) and flavonoids (quercetin glycosides). Four p‐coumaroyl esters were tentatively identified and quantified for the first time in lettuce.ConclusionThe main intact phenolic compounds, including four novel p‐coumaroyl esters, were simultaneously quantified in lettuce with optimal performances and a reduced total time of analysis. These findings make headway in the understanding of the lettuce phytochemicals with potential nutritional relevance.

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Phytochemical Analysis

Tập 22 Số 6

555-563

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