Analysis of Cell Cycle and Morphological and Functional Abnormalities of Mytilus galloprovincialis Lam., 1819 (Bivalvia) Hemocytes from Coastal Ecosystems near Sevastopol, Crimea
Tóm tắt
The Black Sea coastal waters near Sevastopol are used intensively for household and industrial purposes and are characterized by high human disturbance. To investigate the level of environmental toxic impact on the organism functional state, this study analyzes changes in hemolymph cell composition, morphology, DNA content, and cell cycle parameters of bivalve mollusk Mytilus galloprovincialis from six experimental sites. The mollusks from the mussel farm on Donuzlav Lake (Crimea) are the control group. Significant differences have been found between mussels from the control area and coastal waters near Sevastopol in main morphological and functional parameters such as cell size, granularity, and nucleus-cytoplasmic ratio. Mussels from the Sevastopol area are characterized by abnormalities of the hemocyte cell cycle manifested in aneuploidy and an increase in the intraindividual variability of genome size and proportion of cells with 4c DNA content up to 34.80–65.5%, which most likely reflects the cell transition to polyploidy.
Tài liệu tham khảo
Aladaileh, S., Nair, S.V., Birch, D., and Raftos, D.A., Sydney rock oyster (Saccostrea glomerata) hemocytes: morphology and function, J. Invertebr. Pathol., 2007, vol. 96, no. 1, p. 48.
Anisimova, A.A., Morphofunctional parameters of hemocytes in the assessment of the physiological status of bivalves, Russ. J. Mar. Biol., 2013, vol. 39, no. 6, p. 381.
Barcia, R., Cao, A., Arbeteta, J., and Ramos-Martinez, J.I., The IL-2 receptor in hemocytes of the sea mussel Mytilus galloprovincialis Lam, IUBMB Life, 1999, vol. 48, no. 1, p. 419.
Bayne, C.J., Moore, M.N., Carefoo, T.H., and Thompson, R.J., Hemolymph functions in Mytilus californianus: the cytochemistry of hemocytes and their responses to foreign implants and hemolymph factors in phagocytosis, J. Invertebr. Pathol., 1979, vol. 34, no. 1, p. 1.
Bihari, N., Batel, R., and Zahn, R.K., Flow cytometry in marine environmental research, Period. Biol., 1999, vol. 101, no. 2, p. 151.
Bihari, N., Mičić, M., Batel, R., and Zahn, R.K., Flow cytometric detection of DNA cell cycle alterations in hemocytes of mussels (Mytilus galloprovincialis) off the Adriatic coast, Croatia, Aquat. Toxicol., 2003, vol. 64, no. 2, p. 121.
Carballal, M.J., Lopez, C., and Azevedo, C., and Villalba, Hemolymph cell types of the mussel Mytilus galloprovincialis,Dis. Aquat. Org., 1997, vol. 29, no. 2, p. 127.
Delaporte, M., Synard, S., Pariseau, J., McKenna, P., Tremblay, R., Davidson, J., and Berthe, F.C.J., Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry, J. Invertebr. Pathol., 2008, vol. 98, p. 190. https://doi.org/10.1016/j.jip.2007.12.005
Donaghy, L., Lambert, C., Choi, K.-S., and Soudant, P., Hemocytes of the carpet shell clam (Ruditapes decussatus) and the Manila clam (Ruditapes philippinarum): current knowledge and future prospects, Aquaculture, 2009, vol. 297, p. 10.
Elston, R.A., Drum, A.S., and Allen, S.K., Progressive development of circulating polyploidy cells in Mytilus with hemic neoplasia, Dis. Aquat. Org., 1990, vol. 8, p. 51.
Feng, S.Y., Feng, J.S., and Yamasu, T., Roles of Mytilus coruscus and Crassostrea gigas blood cells in defense and nutrition, in Comparative Pathobiology, Boston: Springer, 1977, p. 31.
Galloway, S.M., Chromosome aberrations induced in vitro: mechanisms, delayed expression, and intriguing questions, Environ. Mol. Mutagen., 1994, vol. 23 (suppl.), p. 24. https://doi.org/10.1002/em.2850230612
Giron-Pérez, M.I., Relationships between innate immunity in bivalve molluscs and environmental pollution, Invertebr. Survival J., 2010, vol. 7, p. 149.
Hine, P.M., The inter-relationships of bivalve haemocytes, Fish Shellfish Immunol., 1999, vol. 9, no. 5, p. 367. https://doi.org/10.1006/fsim.1998.0205
Höher, N., Köhler, A., Strand, J., and Broeg, K., Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters, Mar. Environ. Res., 2012, vol. 75, p. 35. https://doi.org/10.1016/j.marenvres.2011.11.003
Kadoshnikov, V.M., Shkapenko, V.V., Gorlitskii, B.A., Smirnova, I.R., and Pisanskaya, Yu.D., Heavy metals in bottom sediments of Sevastopol Bay, Mineral. Zh., 2011, p. 73.
Kopytov, Yu.P., Minkina, N.I., and Samyshev, E.Z., The level of pollution of water and bottom sediments of Sevastopol Bay (Black Sea), Sist. Kontr. Okr. Sredy, 2010, no. 14, p. 199.
Lowcock, L.A., Sharbel, T.F., Bonin, J., Ouellet, M., Rodrigue, J., and Des, GrangesJ.L., Flow cytometric assay for in vivo genotoxic effects of pesticides in green frogs (Rana clamitans), Aquat. Toxicol., 1997, vol. 38, p. 241. https://doi.org/10.1016/S0166-445X(96)00846-6
Metzger, M.J., Villalba, A., Carballal, M.J., Iglesias, D., Sherry, J., Reinisch, C., Muttray, A.F., Baldwin, S.A., and Goff, S.P., Widespread transmission of independent cancer lineages within multiple bivalve species, Nature, 2016, no. 534 (7609), p. 705.
Metzger, M.J., Reinisch, C., Sherry, J., and Goff, S.P., 2015. horizontal transmission of clonal cancer cells causes leukemia in soft-shell clams, Cell, vol. 161, pp. 255–263.
Mičić, M., Bihari, N., and Mlinarič-Raščan, I., Influence of herbicide, 2,4-dichlorophenoxy acetic acid, on haemocyte DNA of in vivo treated mussel, J. Exp. Mar. Biol. Ecol., 2004, vol. 311, p. 157. https://doi.org/10.1016/j.jembe.2004.05.007
Mironov, O.G., Alemov, S.V., Osadchaya, T.S., Guseva, E.V., Mironova, T.O., Murav’eva, I.P., Mironov, O.A., Eninina, L.V., Alifanova, D.A., and Volkov, N.G., Monitoring ekologicheskogo sostoyaniya bukhty Artilleriiskaya (Monitoring the Environmental State of Artilleriiskaya Bay (Sevastopol, Black Sea)), Sevastopol: Chernoe More, 2012.
Moiseenko, O.G., Orekhova, N.A., Polyakova, A.V., Medvedev, E.V., and Konovalov, S.K., Indices and indicators of the environmental status of Sevastopol Bay, Vestn. Mosk. Univ., Ser. 5: Geogr., 2016, no. 4, p. 42.
Moore, J.D., Elsterr, R.A., Drum, S., and Wilkinson, M.T., Alternate pathogenesis of systemic neoplasia in the bivalve mollusk Mytilus,J. Invertebr. Pathol., 1991, vol. 58, p. 231. https://doi.org/10.1016/0022-2011(91)90067-Z
Nunez, R., DNA measurement and cell cycle analysis by flow cytometry, Curr. Iss. Mol. Biol., 2001, vol. 3, pp. 67–70.
Oehlmann, J. and Schulte-Oehlmann, U., Molluscs as bioindicators, Trace Metals Other Contam. Environ., 2003, vol. 6, p. 577.
Orekhova, N.A., Romanov, A.S., and Khoruzhii, D.S., Interannual changes in the concentration of nutrients in Sevastopol Bay in 2006–2010, Kologichna Bezpeka Priberezhnoï ta Shel’fovoï Zon ta Kompleksne Vikoristannya Resursiv Shel’fu, 2011, vol. 1, p. 25.
Osadchaya, T.S., Alemov, S.V., and Shadrina, T.V., Environmental quality of bottom sediments at Sevastopol Bay: retrospective and current state, Ekol. Morya, 2004, vol. 66, p. 82.
Ottaviani, E., Franchini, A., Barbieri, D., and Kletsas, D., Comparative and morphofunctional studies on Mytilus galloprovincialis hemocytes: presence of two aging-related hemocyte stages, Ital. J. Zool., 1998, vol. 65, no. 4, p. 349.
Ovsyanyi, E.I., Kotel’yanets, E.A., and Orekhova, N.A., Arsenic and heavy metals in the sediments of Balaklava Bay (Black Sea), Morsk. Gidrofiz. Zh., 2009, no. 4, p. 67.
Pipe, R.K., Differential binding of lectins to haemocytes of the mussel Mytilus edulis,Cell Tissue Res., 1990, vol. 261, no. 2, p. 261.
Pipe, R.K., Farley, S.R., and Coles, J.A., The separation and characterization of haemocytes from the mussel Mytilus edulis,Cell Tissue Res., 1997, vol. 289, no. 3, p. 537.
Renault, T. and Novoa, B., Viruses infecting bivalve mollusks, Aquat. Living Res., 2014, vol. 17, p. 397.
Reno, P.W., House, M., and Illingworth, A., Flow cytometric and chromosome analysis of softshell clams, Mya arenaria, with disseminated neoplasia, J. Invertebr. Pathol., 1994, vol. 64, p. 163.
Rodríguez, A.M., Torrado, M., and Mendéz, J., Genome-size variation in bivalve molluscs determined by flow cytometry, Mar. Biol. (Berlin), 1996, vol. 126, p. 489.
Russo, J. and Lagadic, L., Effects of environmental concentrations of atrazine on hemocyte density and phagocytic activity in the pond snail Lymnaea stagnalis (Gastropoda, Pulmonata), Environ. Pollut., 2004, vol. 127, p. 303. https://doi.org/10.1016/S0269-7491(03)00269-0
Seiler, G.R. and Morse, M.P., Kidney and hemocytes of Mya arenaria (Bivalvia): normal and pollution-related ultrastructural morphologies, J. Invertebr. Pathol., vol. 52, no. 2, p. 201. https://doi.org/10.1016/0022-2011(88)90127-9
da Silva, P.M., Soudant, P., Carballal, M.J., Soudant, P., Carballal, M.J., Lambert, C., and Villalba, A., Flow cytometric DNA content analysis of neoplastic cells in haemolymph of the cockle Cerastoderma edule,Dis. Aquat. Organ., 2005, vol. 67, p. 133. https://doi.org/10.3354/dao067133
Sung, H.H., Kao, W.Y., and Su, Y.J., Effects and toxicity of phthalate esters to hemocytes of giant freshwater prawn, Macrobrachium rosenbergii,Aquat. Toxicol., 2003, vol. 64, no. 1, p. 25. https://doi.org/10.1016/S0166-445X(03)00011-0
Valli, G., Ciclo di maturita sessuale in Mytilus galloprovincialis Lam. di Duino (Trieste), Boll. Pesca, Piscicolt.Ehidrobiol., 1971, vol. 26, nos. 1–2, p. 259.
Vassilenko, E. and Baldwin, S.A., Using flow cytometry to detect haemic neoplasia in mussels (Mytilus trossulus) from the pacific coast of southern British Columbia, Canada, J. Invertebr. Pathol., 2014, vol. 117, p. 68. https://doi.org/10.1016/j.jip.2014.02.002
Zolotnitskaya, R.P., Methods of hematological studies, in Laboratornye metody issledovaniya v klinike (spravochnik) (Laboratory Research Methods in Clinical Practice (A Handbook)), Moscow: Meditsina, 1987, p. 106.