An update on high-yield hepatocyte isolation methods and on the potential clinical use of isolated liver cells

A.C. Puviani1, Chris Ottolenghi2, B. Tassinari2, P. Pazzi3, E Morsiani4
1Department of Biology, University of Ferrara, Italy
2Department of Biology, Section of General Physiology, University of Ferrara, via L. Borsari 46, I-44100 Ferrara, Italy
3Department of Gastroenterology, Sant'Anna University Hospital, Ferrara - Italy
4Department of Surgery, Sant'Anna University Hospital, Ferrara, Italy.

Tóm tắt

Từ khóa


Tài liệu tham khảo

Arkadopoulos, 1997, Transplantation of isolated hepatocytes prolongs survival and improves blood chemistry in anhepatic rats, Hepatology, 26, 252A

Baur, 1975, Criteria of viability of isolated liver cells, Hoppe-Seyler's Z Physiol Chem, 356, 827, 10.1515/bchm2.1975.356.s1.827

Berry, 1969, High-yield preparation of isolated rat liver parenchymal cells, J Cell Biol, 43, 506, 10.1083/jcb.43.3.506

Berry MN, Edwards AM, Barrit GJ. Isolated Hepatocytes. Preparation, Properties and Applications. Amsterdam: Elsevier, 1991.

Berry, 1992, Techniques for pharmacological and toxicological studies with isolated hepatocyte suspensions, Life Sci, 51, 1, 10.1016/0024-3205(92)90212-8

Blitzer, 1982, Effects of inhibitors of Na+-coupled ion transport on bile acid uptake by isolated rat hepatocytes, Am J Physiol, 243, G48

Blouin, 1977, Distribution of organelles and membranes between hepatocytes and non hepatocytes in the rat liver parenchyma, J Cell Biol, 72, 441, 10.1083/jcb.72.2.441

Bojar, 1976, Preparation of the parenchymal and nonparenchymal cells from adult human liver: morphological and biochemical characteristics, J Clin Biochem, 14, 527

Boyer, 1990, Preparation and specific applications of isolated hepatocyte couplets, Methods Enzymol, 192, 501, 10.1016/0076-6879(90)92090-Z

Demetriou, 1986, Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes, Science, 233, 1190, 10.1126/science.2426782

Demetriou, 1986, New method of hepatocyte transplantation and extracorporeal liver support, Ann Surg, 204, 259, 10.1097/00000658-198609000-00005

Demetriou, 1988, Transplantation of microcarrier-attached hepatocytes into 90% partially hepatectomized rats, Hepatology, 8, 1006, 10.1002/hep.1840080505

Demetriou, 1991, Hepatocyte transplantation. A potential treatment for liver disease, Dig Dis Sci, 36, 1320, 10.1007/BF01307530

Demetriou, 1994, Hepatocyte transplantation, Sci Am Sci Med, 6, 58

Dou, 1992, Thawed human hepatocytes in primary culture, Cryobiology, 29, 454, 10.1016/0011-2240(92)90048-7

Epstein, 1967, Cell size, nuclear content and the development of polyploidy in the mammalian liver, Proc Natl Acad Sci USA, 57, 327, 10.1073/pnas.57.2.327

Fabre, 1988, Characterization of Midazolam metabolism using human hepatic fractions and hepatocytes in suspension obtained by perfusing whole human livers, Biochem Pharmacol, 37, 4389, 10.1016/0006-2952(88)90622-3

Fry, 1981, Preparation of mammalian hepatocytes, Methods Enzymol, 77, 130, 10.1016/S0076-6879(81)77017-4

Graf, 1984, Isolated rat hepatocyte couplets: A primary secretory unit for electrophysiologic studies of bile secretory function, Proc Natl Acad Sci USA, 81, 6516, 10.1073/pnas.81.20.6516

Groth, 1977, Correction of hyperbilirubinemia in the glucoronyltransferase deficient rats by intraportal hepatocyte transplantation, Transplant Proc, 9, 313

Hinton, 1976, Ultrastructure of the liver in channel catfish Ictalurus punctatus (Rafinesque), J Fish Biol, 8, 209, 10.1111/j.1095-8649.1976.tb03944.x

Howard, 1968, Preparation and partial characterization of intact isolated parenchymal cells from rat liver, Biol Chem, 243, 3105, 10.1016/S0021-9258(18)93383-1

Jauregui, 1994, Primary cultures of rat hepatocytes in hollow fiber chambers, In Vitro Cell Dev Biol, 30A, 23, 10.1007/BF02631414

Jungermann, 1989, Functional specialization of different hepatocyte populations, Physiol Rev, 69, 708, 10.1152/physrev.1989.69.3.708

Kaltenbach, 1954, Mechanical preparation of isolated hepatocytes, Exp Cell Res, 7, 568, 10.1016/S0014-4827(54)80101-7

Kreamer, 1986, Use of low-speed, iso-density percoll centrifugation method to increase the viability of isolated rat hepatocyte preparation, In Vitro Cell Dev Biol, 22, 201, 10.1007/BF02623304

Lautenschlager, 1984, Distribution of the major histocompatibility complex antigens on different cellular components of human liver, Cell Immunol, 85, 191, 10.1016/0008-8749(84)90289-2

Leffert, 1979, Hormonal control of rat liver regeneration, Gastroenterology, 76, 1470, 10.1016/0016-5085(79)90418-9

Lindros, 1985, Digitonin collagenase perfusion for efficient separation of periportal or perivenous hepatocytes, Biochemistry, 228, 757, 10.1042/bj2280757

Matas, 1976, Hepatocellular transplantation for metabolic deficiencies: decrease of plasma bilirubin in Gunn rats, Science, 192, 892, 10.1126/science.818706

Makowka, 1980, Cellular transplantation in the treatment of experimental hepatic failure, Science, 210, 901, 10.1126/science.7001630

Mine, 1990, Difference in sensitivity to glucagon action in three different rat liver systems, Metab Clin Exp, 39, 321, 10.1016/0026-0495(90)90054-G

Mito, 1993, Hepatocyte transplantation in man, Cell Transplant, 2, 65, 10.1177/096368979300200109

Morsiani, 1985, Preparation and allotransplantation of isolated hepatocytes in partially hepatectomized rats, Ital J Surg Sci, 15, 23

Morsiani, 1994, Automated large-scale production of porcine hepatocytes for bioartificial liver support, Transplant Proc, 26, 4105

Morsiani, 1995, Automated liver cell processing facilitates large-scale isolation and purification of porcine hepatocytes, ASAIO J, 41, 155, 10.1097/00002480-199541020-00006

Morsiani E, Pazzi P, Moscioni AD, Rozga J, Demetriou AA. Morphological and functional characterization of isolated porcine hepatocytes for bioartificial liver support: bile acid uptake and conjugation, J Surg Res 1998;79:54–60.

Moscioni, 1987, Transplantation of cryopreserved microcarrier-attached human hepatocytes into gunn rats, Surg Forum, 38, 385

Moscioni, 1989, Human liver cell transplantation. Prolonged function in athymic-gunn and athymic-analbuminemic hybrid rats, Gastroenterology, 96, 1546, 10.1016/0016-5085(89)90525-8

Oka, 1992, A modified colorimetric MTT assay adapted for primary cultured hepatocytes: application to proliferation and cytotoxicity assays, Biosci Biotech Biochem, 56, 1472, 10.1271/bbb.56.1472

Oshio, 1992, Contractility of bile canaliculi: implications for liver function, Science, 212, 1041, 10.1126/science.7015506

Otto DA, Cook, GA, Reiss PD. In: Harris RA, Cornell NW, editors. Isolation, Characterization and use of Hepatocytes. New York: Elsevier, 1983:41–48.

Pazzi, 1997, Bile salt-induced cytotoxicity and ursodeoxycholate cytoprotection: in vitro study in perfused rat hepatocytes, Eur J Gastroenterol Hepatol, 9, 703, 10.1097/00042737-199707000-00011

Pertoft, 1977, The viability of cells grown or centrifuged in a density gradient medium, Percoll (TM), Exp Cell Res, 110, 449, 10.1016/0014-4827(77)90311-1

Phillips HJ. Dye exclusion tests for cell viability. In: Kruse PF, Patterson MK, editors. Tissue Culture Methods and Applications. New York: Academic Press, 1973:406–408.

Puviani AC, Tassinari B, Ottolenghi C, Ganzerli S, Gavioli G, Ricci D, Gorini P, Pazzi P, Morsiani E. Development of novel radial-flow bioreactor for biohybrid liver support system. In: Abstract Book, New Frontiers in the Treatment of Liver Diseases. Padua, 5–6 December, 1997:61.

Puviani AC, Tassinari B, Ottolenghi C, Ganzerli S, Gavioli G, Ricci D, Fogli L, Pazzi P, Morsiani E. Three-dimensional culture using polyester fabric system for hepatocytes. In: Abstract Book, New Frontiers in the Treatment of Liver Diseases. Padua, 5–6 December, 1997:60.

Quistorff, 1985, Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartimentation in the liver, Biochem J, 226, 289, 10.1042/bj2260289

Rappaport, 1958, The structural and functional unit of the human liver (liver acinus), Anat Rec, 130, 637, 10.1002/ar.1091300405

Rappaport, 1976, The microcirculatory acinar concept of normal and pathological hepatic structure, Beitr Pathol, 157, 215, 10.1016/S0005-8165(76)80083-2

Renton, 1978, Effects of polyriboinosinic acid-polyribocytidylic acid and a mouse interferon preparation on cytochrome P-450-dependent monooxygenase systems in cultures of primary mouse hepatocytes, Mol Pharmachol, 14, 672

Rivas, 1993, The morphology and function of rabbit hepatocytes isolated using ethylene-diaminetetracetate, Transplantation, 55, 335, 10.1097/00007890-199302000-00020

Rozga, 1993, Development of bioartificial liver: properties and function of a hollow-fiber module inoculated with liver cells, Hepatology, 17, 258, 10.1002/hep.1840170216

Rozga, 1994, A bioartificial liver to treat severe acute liver failure, Ann Surg, 219, 538, 10.1097/00000658-199405000-00012

Rozga, 1994, Isolated hepatocytes in a bioartificial liver: a single group view and experience, Biotechnol Bioeng, 43, 645, 10.1002/bit.260430714

Sachs, 1994, The pig as a potential xenograft donor, Vet Immunol Immunopathol, 43, 185, 10.1016/0165-2427(94)90135-X

Schiller, 1992, Assessment of viability of hepatocytes in suspension using the MTT assay, Toxicol Vitro, 6, 575, 10.1016/0887-2333(92)90070-8

Schneider, 1943, Hepatocyte preparation by mechanical disruption, J Biol Chem, 149, 217, 10.1016/S0021-9258(18)72232-1

Seglen, 1972, Properties of rat liver cells. I. Effect of Ca++ on enzymatic dispersion of isolated perfused liver, Exp Cell Res, 74, 450, 10.1016/0014-4827(72)90400-4

Seglen, 1976, Preparation of isolated rat liver cells, Methods Cell Biol, 13, 29, 10.1016/S0091-679X(08)61797-5

Sutherland, 1977, Hepatocellular transplantation in acute liver failure, Surgery, 82, 124

Talamini, 1997, Repolarization of hepatocytes in culture, Hepatology, 25, 167, 10.1002/hep.510250131

Tonda, 1983, Effects of phenobarbital and 3-methylcholantrene pretreatments on mono-oxygenase activities and proportions of isolated rat hepatocyte subpopulations, Mol Pharmacol, 23, 235

Walpole, 1990, Rabbit hepatocytes in primary culture: preparation, viability and use in studies of propanolol metabolism, Hepatology, 11, 394, 10.1002/hep.1840110310

Wang, 1985, Isolation of rat hepatocytes with EDTA and their metabolic function in primary culture, In Vitro Cell Dev Biol, 21, 526, 10.1007/BF02620846

Watanabe, 1997, Clinical experience with a bioartificial liver in the treatment of severe liver failure. A phase I clinical trial, Ann Surg, 225, 484, 10.1097/00000658-199705000-00005

Wiederkehr, 1990, Hepatocyte transplantation for the low-density lipoprotein receptor-deficient state. A study in the Watanabe rabbit, Transplantation, 50, 466, 10.1097/00007890-199009000-00021

Yarmush, 1992, Assessment of artificial liver support technology, Cell Transplant, 1, 323, 10.1177/096368979200100501