Vaccine cúm A dựa trên virus alpha được điều chỉnh M2e

Archives of Virology - Tập 163 - Trang 483-488 - 2017
Haritha Ramya Krishnavajhala1, Jacqueline Williams1, Hans Heidner1
1Department of Biology, The University of Texas at San Antonio, San Antonio, USA

Tóm tắt

Miền bên ngoài 23-residue của protein M2 virus cúm A (M2e) có tiềm năng lớn như một kháng nguyên vắc-xin. Ở đây, chúng tôi mô tả việc xây dựng và đặc trưng hóa virus Sindbis điều chỉnh M2e được chỉ định là E2S1-M2e. Các virion E2S1-M2e chứa M2e như một phần mở rộng N-giá của glycoprotein E2 và do đó biểu hiện 240 bản sao của peptide M2e trên bề mặt của chúng. Virus E2S1-M2e đã biểu hiện M2e ở dạng dễ tiếp cận và có khả năng gây miễn dịch, và đã kích thích sản xuất kháng thể đặc hiệu với M2e khi được tiêm cho chuột. Những con chuột nhận vắc-xin qua mũi với E2S1-M2e đã được bảo vệ chống lại thử thách gây chết với một chủng virus cúm A độc lực, đã thích nghi cho chuột.

Từ khóa

#virus cúm A #vaccine #M2e #virus Sindbis #kháng thể đặc hiệu

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