An improved soft agar method for determining neoplastic transformation in vitro

Fumio Suzuki1, Keiji Suzuki1, Osamu Nikaido1
1Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan

Tóm tắt

Từ khóa


Tài liệu tham khảo

Freedman, V. H.; Shin, S-I. Cellular tumorigenicity innude mice: Correlation with cell growth in semi-solid medium. Cell 3: 355–359; 1974.

Shin, S-I.; Freedman, V. H.; Risser, R.; Pollack, R. Tumorigenicity of virus-transformed cells innude mice is correlated specifically with anchorage independent growthin vitro. Proc. Natl. Acad. Sci. USA 72: 4435–4439; 1975.

Jones, P. A.; Laug, W. E.; Gardner, A.; Nye, C. A.; Fink, L. M.; Benedict, W. F.In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells. Cancer Res. 36: 2863–2867; 1976.

Colburn, N. H.; Bruegge, W. F. V.; Bates, J. R.; Gray, R. H.; Rossen, J. D.; Kelsey, W. H.; Shimada, T. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624–634; 1978.

Otsu, H.; Yasukawa, M.; Terasima, T.In vitro properties and tumorigenicity of radiation-transformed clones of mouse 10T1/2 cells. J. Radiat. Res. 24: 118–130; 1983.

Macpherson, I.; Montagnier, L. Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23: 291–294; 1964.

Maher, V. M.; Rowan, L. A.; Silinskas, K. C.; Kateley, S. A.; McCormick, J. J. Frequency of UV-induced neoplastic transformation of diploid human fibroblasts is higher in xeroderma pigementosum cells than in normal cells. Proc. Natl. Acad. Sci. USA 79: 2613–2617; 1982.

Milo, G. E. Jr; DiPaolo, J. A. Neoplastic transformation of human diploid cellsin vitro after chemical carcinogen treatment. Nature 275: 130–132; 1978.

Silinskas, K. C.; Kateley, S. A.; Tower, J. E.; Maher, V. M.; McCormick, J. J. Induction of anchorage-independent growth in human fibroblasts by propanesultone. Cancer Res. 41: 1620–1627; 1981.

Zimmerman, R. J.; Little, J. B. Characterization of a quantitative assay for thein vitro transformation of normal human diploid fibroblasts to anchorage independence by chemical carcinogens. Cancer Res. 43: 2176–2182; 1983.

Kakunaga, T. Neoplastic transformation of human diploid fibroblast cells by chemical carcinogens. Proc. Natl. Acad. Sci. USA 75: 1334–1338; 1978.

Bouck, N.; Mayorca, G. D. Somatic mutation as the basis for malignant transformation of BHK cells by chemical carcinogens. Nature 264: 722–727; 1976.

Dulbecco, R.; Vogt, M. Plaque formation and isolation of pure lines with poliomyelitis virus. J. Exp. Med. 99: 167–182; 1954.

Macpherson, I. Soft agar techniques. In: Kruse, P. F., Jr.; Patterson, M. K., Jr., eds. Tissue culture: methods and applications. New York: Academic Press; 1973, 276–280.

Leavitt, J. C.; Crawford, B. D.; Barrett, J. C.; Ts'o, P. O. P. Regulation of requirements for anchorage-independent growth of Syrian hamster fibroblasts by somatic mutation. Nature 269: 63–65; 1977.