An improved PCR-based amplification of unknown homologous DNA sequences

Barnes, 1994, PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates, Proc. Natl. Acad. Sci. USA, 91, 2216, 10.1073/pnas.91.6.2216 Baumforth, 1999, Demystified… the polymerase chain reaction, Mol. Pathol., 52, 1, 10.1136/mp.52.1.1 Bi, 1998, Detection of known mutation by proof-reading PCR, Nucleic Acids Res., 26, 3073, 10.1093/nar/26.12.3073 Cahill, 2003, Exo-proofreading, a versatile SNP scoring technology, Genome Res., 13, 925, 10.1101/gr.939903 Cheng, 1994, Effective amplification of long targets from cloned inserts and human genomic DNA, Proc. Natl. Acad. Sci. USA, 91, 5695, 10.1073/pnas.91.12.5695 Ehlers, 1999, Detection of new DNA polymerase genes of known and potentially novel herpesviruses by PCR with degenerate and deoxyinosine-substituted primers, Virus Genes, 18, 211, 10.1023/A:1008064118057 Kilpatrick, 1996, Group-specific identification of polioviruses by PCR using primers containing mixed-base or deoxyinosine residue at positions of codon degeneracy, J. Clin. Microbiol., 34, 2990, 10.1128/JCM.34.12.2990-2996.1996 Lam, 2006, Allele dropout in PCR-based diagnosis of Wilson disease: mechanisms and solutions, Clin. Chem., 52, 517, 10.1373/clinchem.2005.060491 Lundberg, 1991, High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus, Gene, 108, 1, 10.1016/0378-1119(91)90480-Y Mitsuhashi, 1996, Technical report. Part 2. Basic requirements for designing optimal PCR primers, J. Clin. Lab. Anal., 10, 285, 10.1002/(SICI)1098-2825(1996)10:5<285::AID-JCLA9>3.0.CO;2-7 Rossolini, 1994, Use of deoxyinosine-containing primers vs degenerate primers for polymerase chain reaction based on ambiguous sequence information, Mol. Cell Probes, 8, 91, 10.1006/mcpr.1994.1013