AnUstilago maydischassis for itaconic acid production without by‐products

Microbial Biotechnology - Tập 13 Số 2 - Trang 350-362 - 2020
Johanna Becker1, Hamed Hosseinpour Tehrani1, Marc Gauert2, Jörg Mämpel2, Lars M. Blank1, Nick Wierckx1,3
1Institute of Applied Microbiology – iAMB, Aachen Biology and Biotechnology – ABBt, RWTH Aachen University, Worringerweg 1, Aachen, 52074, Germany
2BRAIN AG (Biotechnology, Research and Information Network) Darmstädter Str. 34‐36 Zwingenberg 64673 Germany
3Institute of Bio‐ and Geosciences IBG‐1: Biotechnology Forschungszentrum Jülich Jülich 52425 Germany

Tóm tắt

SummaryUstilago maydisis a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by‐product spectrum ofU. maydiswas reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker‐free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by‐product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.

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Microbial Biotechnology

Tập 13 Số 2

350-362

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