An <i>Arabidopsis</i> mutation in translation elongation factor 2 causes superinduction of <i>CBF/DREB1</i> transcription factor genes but blocks the induction of their downstream targets under low temperatures

Proceedings of the National Academy of Sciences of the United States of America - Tập 99 Số 11 - Trang 7786-7791 - 2002
Yan Guo1, Liming Xiong1, Manabu Ishitani1, Jian‐Kang Zhu1
1Department of Plant Sciences, University of Arizona, Tucson, AZ 85721

Tóm tắt

Low temperature regulates gene expression in bacteria, yeast, and animals as well as in plants. However, the signal transduction cascades mediating the low temperature responses are not well understood in any organism. To identify components in low temperature signaling genetically, we isolated Arabidopsis thaliana mutants in which cold-responsive genes are no longer induced by low temperatures. One of these mutations, los1–1 , specifically blocks low temperature-induced transcription of cold-responsive genes. Surprisingly, cold-induced expression of the early response transcriptional activators, C-repeat/dehydration responsive element binding factors (CBF/DREB1s), is enhanced by the los1–1 mutation. The los1–1 mutation also reduces the capacity of plants to develop freezing tolerance but does not impair the vernalization response. Genetic analysis indicated that los1–1 is a recessive mutation in a single nuclear gene. The LOS1 gene encodes a translation elongation factor 2-like protein. Protein labeling studies show that new protein synthesis is blocked in los1–1 mutant plants specifically in the cold. These results reveal a critical role of new protein synthesis in the proper transduction of low temperature signals. Our results also suggest that cold-induced transcription of CBF/DREB1 s is feedback inhibited by their gene products or by products of their downstream target genes.

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Tài liệu tham khảo

10.1007/978-3-642-79133-8_29

10.1146/annurev.arplant.50.1.571

10.1104/pp.84.3.872

10.1104/pp.93.3.1246

10.1007/BF00016077

10.1073/pnas.93.23.13404

10.1073/pnas.95.24.14570

K Yamaguchi-Shinozaki, K Shinozaki Plant Cell 6, 251–264 (1994).

10.1073/pnas.94.3.1035

10.1105/tpc.10.8.1391

10.1126/science.280.5360.104

M Ishitani, L Xiong, B Stevenson, J-K Zhu Plant Cell 9, 1935–1949 (1997).

10.1101/gad.866801

L Xiong, M Ishitani, H Lee, J K Zhu Plant Cell 9, 2063–2083 (2001).

10.1007/BF01379368

10.1104/pp.114.2.591

10.1104/pp.99.2.519

10.1007/BF00018452

10.1093/oxfordjournals.pcp.a078243

10.1104/pp.125.2.770

10.1016/0378-1119(93)90266-6

10.1007/978-1-4612-2598-0_8

10.1046/j.1365-313X.1993.04020403.x

10.1006/geno.1994.1023

10.1002/j.1460-2075.1994.tb06675.x

10.1093/emboj/16.13.4092

10.1007/BF00986958

, eds B Alberts, D Bray, J Lewis, M Raff, K Roberts, J D Watson (Garland, New York) Molecular Biology of the Cell, pp. 729–731 (1994).

10.1046/j.1365-313x.1998.00310.x

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Proceedings of the National Academy of Sciences of the United States of America

Tập 99 Số 11

7786-7791

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