An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities

Bjourson, 1992, Combined subtraction hybridization and polymerase chain reaction amplification procedure for isolation of strain-specific Rhizobium DNA sequences, Appl. Environ. Microbiol., 58, 2296, 10.1128/AEM.58.7.2296-2301.1992 Braker, 2001, Community structure of denitrifiers, bacteria, and archaea along redox gradients in Pacific Northwest marine sediments by terminal restriction fragment polymorphism analysis of amplified nitrite reductase (nirS) and 16S rRNA genes, Appl. Environ. Microbiol., 67, 1893, 10.1128/AEM.67.4.1893-1901.2001 Buchaille, 2000, A substractive PCR-based cDNA library from human odontoblast cells: identification of novel genes expressed in tooth forming cells, Matrix Biol., 19, 421, 10.1016/S0945-053X(00)00091-3 Diez, 2001, Application of denaturing gradient gel electrophoresis (DGGE) to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques, Appl. Environ. Microbiol., 67, 2942, 10.1128/AEM.67.7.2942-2951.2001 Dunbar, 1999, Levels of bacterial community diversity in four arid soils compared by cultivation and 16S rRNA gene cloning, Appl. Environ. Microbiol., 65, 1662, 10.1128/AEM.65.4.1662-1669.1999 Gich, 2001, Previously unknown and phylogenetically diverse members of the green nonsulfur bacteria are indigenous to freshwater lakes, Arch. Microbiol., 177, 1, 10.1007/s00203-001-0354-6 Hugenholtz, 1998, Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity, J. Bacteriol., 180, 4756, 10.1128/JB.180.24.6793-6793.1998 Hughes, 2001, Counting the uncountable: statistical approaches to estimating microbial diversity, Appl. Environ. Microbiol., 67, 4399, 10.1128/AEM.67.10.4399-4406.2001 Jackson, 2001, Molecular analysis of microbial community structure in an arsenite-oxidizing acidic thermal spring, Environ. Microbiol., 3, 532, 10.1046/j.1462-2920.2001.00221.x Liu, 1997, Characterization of microbial diversity by determining terminal restriction length polymorphisms of genes encoding 16S rDNA, Appl. Environ. Microbiol., 63, 4516, 10.1128/AEM.63.11.4516-4522.1997 Massana, 1997, Vertical distribution and phylogenetic characterization of marine planktonic archaea in the Santa Barbara channel, Appl. Environ. Microbiol., 63, 50, 10.1128/AEM.63.1.50-56.1997 Muyzer, 1993, Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA, Appl. Environ. Microbiol., 59, 695, 10.1128/AEM.59.3.695-700.1993 Muyzer, 1996, Denaturing gradient gel electrophoresis of PCR amplified 16S rDNA—a new molecular approach to analyze the genetic diversity of mixed microbial communities, 1 Orphan, 2001, Comparative analysis of methane-oxidizing archaea and sulfate-reducing bacteria in anoxic marine sediments, Appl. Environ. Microbiol., 67, 1922, 10.1128/AEM.67.4.1922-1934.2001 Pace, 1997, A molecular view of microbial diversity and the biosphere, Science, 276, 734, 10.1126/science.276.5313.734 Roszak, 1987, Survival strategies of bacteria in the natural environments, Microbiol. Rev., 51, 365, 10.1128/mr.51.3.365-379.1987 Sambrook, 1989 Schabereiter-Gurtner, 2001, An advanced molecular strategy to identify bacterial communities on art objects, J. Microbiol. Methods, 45, 77, 10.1016/S0167-7012(01)00227-5 Tsai, 1991, Rapid method for direct extraction of DNA from soils and sediments, Appl. Environ. Microbiol., 57, 1070, 10.1128/AEM.57.4.1070-1074.1991 Vetriani, 1998, Recovery and phylogenetic analysis of archaeal rRNA sequences from continental shelf sediments, FEMS Microbiol. Lett., 161, 83, 10.1111/j.1574-6968.1998.tb12932.x Ward, 1990, 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community, Nature, 345, 63, 10.1038/345063a0 Zwart, 1998, Divergent members of the bacterial division Verrucomicrobiales in a temperate freshwater lake, FEMS Microbiol. Ecol., 25, 159, 10.1111/j.1574-6941.1998.tb00469.x