An efficient in vitro plant regeneration system for the medicinal plant Teucrium stocksianum Boiss.
Tóm tắt
An efficient and rapid plant regeneration system via direct organogenesis was established for Teucrium stocksianum Boiss. (Lamiaceae), an endangered and valuable medicinal plant. Hypocotyl explants excised from seedlings germinated in vitro were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of kinetin and indoleacetic acid (IAA) to induce shoot formation. Differentiation of multiple shoots was initiated within 3 weeks of culture. Optimal regeneration was achieved on medium containing 3 mg/l kinetin and 0.5 mg/l IAA. This particular medium composition significantly improved the production of multiple shoots directly from hypocotyl explants compared to other combinations of plant growth regulators. Root induction was achieved on half-strength MS medium containing indole-3-butyric acid. Rooted plantlets were successfully acclimatized, with a survival rate of 75–80%. The protocol developed in this study could be used for long-term in vitro conservation and mass propagation of this species.
Tài liệu tham khảo
Ahmad H, Ahmad A, Jan MM (2002) The medicinal plants of salt range. J Biol Sci 2:175–177
Chen CC, Chen SJ, Sagare AP, Tsay HS (2001) Adventitious shoot regeneration from stem internode explants of Adenophora triphylla (Thunb.) A. DC. (Campanulaceae)—an important medicinal herb. Bot Bull Acad Sin 42:1–7
Debnath M, Malik CP, Bisen PS (2006) Micropropagation: a tool for the production of high quality plant-based medicines. Curr Pharm Biotechnol 7:33–49
Fay MF (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant 28:1–4
Huang CL, Hsieh MT, Hsieh WC, Sagare AP, Tsay HS (2000) In vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot tip, leaf- and inflorescence-node explants. In Vitro Cell Dev Biol Plant 36:220–224
Iqbal I, Hamayun M (2004) Studies on the traditional uses of plants of Malam Jabba valley, District Swat, Pakistan. Ethnobotanical Leaflets; International Web Journal, pp 1–30, 12 May 2006. http://www.siu.edu/∼ebl/index2004.htm
Islam MW, Zakaria MNM, Radhakrishnan R, Kamil M, Chan KC, Al-Attas A (2002) Effect of Teucrium stocksianum on gastric ulceration and secretion in rats. Pharm Biol 40:216–220
Jayaram K, Prasad MNV (2007) Rapid in vitro multiplication of Drosera indica L.: a vulnerable, medicinally important insectivorous plant. Plant Biotechnol Rep 1:79–84
Jogbloed MVD (2003) The comprehensive guide to the wild flowers of the United Arab Emirates. Environmental Research for Wildlife Development Agency, United Arab Emirates
Joshi M, U Dhar (2003) In vitro propagation of Saussurea obvallata (DC.) Edgew.—an endangered ethnoreligious medicinal herb of Himalaya. Plant Cell Rep 21:933–939
Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2003) Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21:525–530
Makunga NP, Jager AK, Staden J van (2003) Micropropagation of Thapsia gargania—a medicinal plant. Plant Cell Rep 21:967–973
Martin KP (2003) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21:415–420
Mojab F, Javidnia K, Yazdani D, Rustaiynan A (2003) Essential oil of the aerial parts of Teucrium stocksianum Boiss subsp. stocksianum (Lamiacea) from Iran. J Med Plants 2:49–53
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nadaf SK, Al-Farsi SM, Al-Hinai SA (2003) Germplasm collection of rangeland forage and medicinal plant species in north Oman. In: Annu Rep ICARDA–APRP. ICARDA–APRP, Dubai, pp 63–64
Radhakrishnan R, Zakaria MNM, Islam MW, Kamil M, Ismail A, Chan K, Al-Attas A (2001) Analgesic and anti-inflammatory activities of Teucrium stocksianum. Pharm Biol 39:455–459
Rasheed RA, Ali BH, Bachir AK (1995) Effect of Teucrium stocksianum on paracetamol-induced hepatotoxicity in mice. Gen Pharmacol 26:297–301
Schmeda-Hirschmann G, Jordan M, Gerth A, Wilken D, Hormazabal E, Tapia AA (2004) Secondary metabolite content in Fabiana imbricata plants and in vitro cultures. Z Naturforsch 59:48–54
Shinwari ZK, Gilani SS (2003) Sustainable harvest of medicinal plants of Astore, Northern Pakistan. J Ethnopharmacol 2:289–298
Van Huylenbroeck CJM, Debergh PC (1995) Physiological aspects in acclimatization of micropropagated plants. Plant Tissue Biotechnol 2:136–141
Van Huylenbroeck JM, Piqueras A, Debergh PC (1998) Photosynthesis and carbon metabolism in leaves formed prior and during ex vitro acclimatization of micropropagated plants. Plant Sci 137:21–30
Wawrosh PR, Kopp MB (2001) Clonal propagation of Lilium nepalense D Don., a threatened medicinal plant of Nepal. Plant Cell Rep 20:285–288
Western AR (1989) The flora of the United Arab Emirates, an introduction. United Arab Emirates University, Al Ain
Xu X, Yu R, Zhao Y (2004) Establishment of plantlet regeneration system of medicinal plant Aconitum coreanum. Zhong Yao Cai 27:797–799
Yamada Y, Shoyama Y, Nishioka I, Kohda H, Namera A, Okamoto T (1991) Clonal micropropagation of Gentiana scabra Bunge var. buergeri Maxim. and examination of the homogeneity concerning the gentiopicroside content. Chem Pharm Bull 39:204–206
You XL, Han JY, Choi YE (2007) Plant regeneration via direct somatic embryogenesis in Panax japonicus. Plant Biotechnol Rep 1:5–9