An autoradiographic study of human blood basophils

Springer Science and Business Media LLC - Tập 7 - Trang 357-360 - 1975
M. R. Parwaresch1, D. Arndt1
1Department of General Pathology, Centre of Clinical and Theoretical Medicine I, Christian-Albrechts-University Kiel, Kiel, West Germany

Tóm tắt

The aim of this paper is the selective visualization of human blood basophils in autoradiographs. [3H]Thymidine-labelled basophils in buffy coat smears were fixed in methanol-formaldehyde followed by 5-aminoacridine hydrochloride and stained with basic Aldehyde Fuchsin prior to autoradiographic processing. The technique described represents a simple method for quantitative autoradiographic studies on basophil kinetics and the interaction of these cells with IgE-mediated atopias.

Tài liệu tham khảo

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