An aptamer that neutralizes R5 strains of HIV-1 binds to core residues of gp120 in the CCR5 binding site

Virology - Tập 381 Số 1 - Trang 46-54 - 2008
C. Cohen1, Mario Forzan1, Brian S. Sproat2, Ralph Pantophlet3, Ian McGowan4, Dennis R. Burton5, William James1
1Sir William Dunn School of Pathology, University of Oxford, OX1 3RE, UK
2Integrated DNA Technologies, BVBA, Interleuvenlaan 12A, B-3001 Leuven, Belgium
3Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
4Magee Women's Research Institute, University of Pittsburgh, 204 Craft Avenue Pittsburgh, PA 15213, USA
5Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA

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Tài liệu tham khảo

Baba, 1999, A small-molecule, nonpeptide CCR5 antagonist with highly potent and selective anti-HIV-1 activity, Proc. Natl. Acad. Sci. U. S. A., 96, 5698, 10.1073/pnas.96.10.5698

Burton, 1991, A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals, Proc. Natl. Acad. Sci. U. S. A., 88, 10134, 10.1073/pnas.88.22.10134

Butler, 2001, A quantitative assay for HIV DNA integration in vivo, Nat. Med., 7, 631, 10.1038/87979

Chen, 2005, Structure of an unliganded simian immunodeficiency virus gp120 core, Nature, 433, 834, 10.1038/nature03327

Cohen, 2008, AIDS research. Microbicide fails to protect against HIV, Science, 319, 1026, 10.1126/science.319.5866.1026b

Cormier, 2002, The crown and stem of the V3 loop play distinct roles in human immunodeficiency virus type 1 envelope glycoprotein interactions with the CCR5 coreceptor, J. Virol., 76, 8953, 10.1128/JVI.76.17.8953-8957.2002

Cormier, 2001, Mapping the determinants of the CCR5 amino-terminal sulfopeptide interaction with soluble human immunodeficiency virus type 1 gp120–CD4 complexes, J. Virol., 75, 5541, 10.1128/JVI.75.12.5541-5549.2001

Darbha, 2004, Crystal structure of the broadly cross-reactive HIV-1-neutralizing Fab X5 and fine mapping of its epitope, Biochemistry, 43, 1410, 10.1021/bi035323x

de Parseval, 2005, A highly conserved arginine in gp120 governs HIV-1 binding to both syndecans and CCR5 via sulfated motifs, J. Biol. Chem., 280, 39493, 10.1074/jbc.M504233200

Dey, 2005, Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5 strains of HIV-1, RNA, 11, 873, 10.1261/rna.7205405

Dey, 2005, An aptamer that neutralizes R5 strains of human immunodeficiency virus type 1 blocks gp120–CCR5 interaction, J. Virol., 79, 13806, 10.1128/JVI.79.21.13806-13810.2005

Huang, 2005, Structure of a V3-containing HIV-1 gp120 core, Science, 310, 1025, 10.1126/science.1118398

Huang, 2007, Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4, Science, 317, 1930, 10.1126/science.1145373

Iribarren, 1990, 2′-O-alkyl oligoribonucleotides as antisense probes, Proc. Natl. Acad. Sci. U. S. A., 87, 7747, 10.1073/pnas.87.19.7747

James, 2007, Aptamers in the virologists' tool kit, J. Gen. Virol., 2007, 351, 10.1099/vir.0.82442-0

Khati, 2003, Neutralization of infectivity of diverse R5 clinical isolates of human immunodeficiency virus type 1 by gp120-binding 2′F-RNA aptamers, J. Virol., 77, 12692, 10.1128/JVI.77.23.12692-12698.2003

Kwong, 1998, Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody, Nature, 393, 648, 10.1038/31405

Labrijn, 2003, Access of antibody molecules to the conserved coreceptor binding site on glycoprotein gp120 is sterically restricted on primary human immunodeficiency virus type 1, J. Virol., 77, 10557, 10.1128/JVI.77.19.10557-10565.2003

Lamond, 1993, Antisense oligonucleotides made of 2′-O-alkylRNA: their properties and applications in RNA biochemistry, FEBS Lett., 325, 123, 10.1016/0014-5793(93)81427-2

Moulard, 2002, Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120–CD4–CCR5 complexes, Proc. Natl. Acad. Sci. U. S. A., 99, 6913, 10.1073/pnas.102562599

Padma, 2008, After microbicide failures, hope that antiviral approach will gel, Nat. Med., 14, 354, 10.1038/nm0408-354a

Pantophlet, 2006, GP120: target for neutralizing HIV-1 antibodies, Annu. Rev. Immunol., 24, 739, 10.1146/annurev.immunol.24.021605.090557

Pantophlet, 2003, Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120, J. Virol., 77, 642, 10.1128/JVI.77.1.642-658.2003

Rizzuto, 2000, Fine definition of a conserved CCR5-binding region on the human immunodeficiency virus type 1 glycoprotein 120, AIDS Res. Hum. Retrovir., 16, 741, 10.1089/088922200308747

Rizzuto, 1998, A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding, Science, 280, 1949, 10.1126/science.280.5371.1949

Scanlan, 2002, The broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2G12 recognizes a cluster of alpha1→2 mannose residues on the outer face of gp120, J. Virol., 76, 7306, 10.1128/JVI.76.14.7306-7321.2002

Sproat, 2005, RNA synthesis using 2′-O-(tert-butyldimethylsilyl) protection, Methods Mol. Biol., 288, 17

Thali, 1993, Characterization of conserved human immunodeficiency virus type 1 gp120 neutralization epitopes exposed upon gp120–CD4 binding, J. Virol., 67, 3978, 10.1128/jvi.67.7.3978-3988.1993

Wyatt, 1995, Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 epitopes induced by receptor binding, J. Virol., 69, 5723, 10.1128/jvi.69.9.5723-5733.1995

Zuker, 2003, Mfold web server for nucleic acid folding and hybridization prediction, Nucleic Acids Res., 31, 3406, 10.1093/nar/gkg595