An Erwinia amylovora uracil transporter mutant retains virulence on immature apple and pear fruit

Microbial Pathogenesis - Tập 147 - Trang 104363 - 2020
Amanda J. Bittner1, Regan B. Huntley2, George S. Mourad1, Neil P. Schultes2
1Department of Biology, Purdue University Fort Wayne, 2101 East Coliseum Blvd., Fort Wayne, IN, 46805, USA
2Department of Plant Pathology & Ecology, The Connecticut Agricultural Experiment Station, 123 Huntington St, New Haven, CT, 06511, USA

Tài liệu tham khảo

Burrill, 1880, Anthrax of fruit trees; or the so-called fire blight of pear, and twig blight of apple trees, Proc. Am. Assoc. Adv. Sci., 2, 583 van der Zwet, 2012 Smits, 2014, Whole-genome sequencing of Erwinia amylovora strains from Mexico detects single nucleotide polymorphisms in rpsL conferring streptomycin resistance and in the avrRpt2 effector altering host interactions, Genome Announc., 2, 10.1128/genomeA.01229-13 Emeriewen, 2019, Malus hosts–Erwinia amylovora interactions: strain pathogenicity and resistance mechanisms, Front. Plant Sci., 10.3389/fpls.2019.00551 Piqué, 2015, Virulence factors of Erwinia amylovora: a review, Int. J. Mol. Sci., 16, 12836, 10.3390/ijms160612836 Schröpfer, 2018, A single effector protein, AvrRpt2EA, from Erwinia amylovora can cause fire blight disease symptoms and induces a salicylic acid-dependent defense response, Mol. Plant Microbe Interact., 31, 1179, 10.1094/MPMI-12-17-0300-R Wöhner, 2018, Inoculation of Malus genotypes with a set of Erwinia amylovora strains indicates a gene-for-gene relationship between the effector gene eop1 and both Malus floribunda 821 and Malus ‘Evereste’, Plant Pathol., 67, 938, 10.1111/ppa.12784 Eastgate, 1997, Identification of a nonpathogenic Erwinia amylovora guaB mutant, Plant Pathol. (Oxf.), 46, 594, 10.1046/j.1365-3059.1997.d01-49.x Wang, 2006, Application of signature-tagged mutagenesis to the study of virulence of Erwinia amylovora, FEMS Microbiol. Lett., 265, 164, 10.1111/j.1574-6968.2006.00476.x Ramos, 2014, Mutation of the Erwinia amylovora argD gene causes arginine autotrophy, nonpathogenicity in apples and reduced virulence in pears, Appl. Environ. Microbiol., 80, 6739, 10.1128/AEM.02404-14 Ramos, 2015, Erwinia amylovora pyrC mutant causes fire blight despite pyrimidine auxotrophy, Lett. Appl. Microbiol., 60, 572, 10.1111/lam.12417 Klee, 2019, Erwinia amylovora auxotrophic mutant exometabolomics and virulence on apples, Appl. Environ. Microbiol., 85, 10.1128/AEM.00935-19 Kourkoulou, 2018, Nucleobase-Ascorbate-transporter (NAT) family Patching, 2018, Recent developments in nucleobase cation symporter-1 (NCS1) family transport proteins from bacteria, archaea, fungi and plants, J. Biosci., 43, 797, 10.1007/s12038-018-9780-3 Papakostas, 2013, Functional identification of the hypoxanthine/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12, J. Biol. Chem., 288, 36827, 10.1074/jbc.M113.523340 Karatza, 2005, Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli, Mol. Membr. Biol., 22, 251, 10.1080/09687860500092927 Papakostas, 2012, Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli, J. Biol. Chem., 287, 15684, 10.1074/jbc.M112.355818 Lu, 2011, Structure and mechanism of the uracil transporter UraA, Nature, 472, 243, 10.1038/nature09885 Botou, 2018, Insite on the specificity of uracil permeases of the NAT/NCS2 family from analysis of the transporter encoded in the pyrimidine utilization operon of Escherichia coli, Mol. Membr. Biol., 108, 204 Yu, 2017, Dimeric structure of the uracil:proton symporter UraA provides mechanistic insights into the SLC4/23/26 transporters, Cell Res., 27, 1020, 10.1038/cr.2017.83 Baba, 2006, Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection, Mol. Syst. Biol., 2, 1, 10.1038/msb4100050 Burse, 2004, The phytoalexin-inducible multi-drug efflux pump, AcrAB, contributes to virulence in the fire blight pathogen, Erwinia amylovora, Mol. Plant Microbe Interact., 17, 43, 10.1094/MPMI.2004.17.1.43 Altschul, 1990, Basic local alignment search tool, J. Mol. Biol., 215, 403, 10.1016/S0022-2836(05)80360-2 Datsenko, 2000, One-step inactivation of chromosomal genes for Escherichia coli K-12 using PCR products, Proc. Natl. Acad. Sci. U.S.A., 97, 6640, 10.1073/pnas.120163297 Klee, 2018, An Erwinia amylovora yjeK mutant exhibits reduced virulence, increased chemical sensitivity and numerous environmentally dependent proteomic alterations, Mol. Plant Pathol., 19, 1667, 10.1111/mpp.12650 Smits, 2010, Complete genome sequence of the fire blight pathogen Erwinia amylovora CFBP 1430 and comparison to other Erwinia spp, Mol. Plant Microbe Interact., 23, 384, 10.1094/MPMI-23-4-0384 Dereeper, 2008, Phylogeny.fr: robust phylogenetic analysis for the non-specialist, Nucleic Acids Res., 1, W465, 10.1093/nar/gkn180 Edgar, 2004, MUSCLE: multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Res., 32, 1792, 10.1093/nar/gkh340 Guindon, 2003, A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood, Syst. Biol., 52, 696, 10.1080/10635150390235520 Ronquist, 2003, MrBayes 3. Bayesian phylogenetic inference under mixed models, Bioinformatics, 19, 572, 10.1093/bioinformatics/btg180 Thompson, 1994, Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res., 22, 4673, 10.1093/nar/22.22.4673 Koukaki, 2005, The nucleobase ascorbate transporter (NAT) signature motif in UapA defines the function of the purine translocation pathway, J. Mol. Biol., 350, 499, 10.1016/j.jmb.2005.04.076 Borruso, 2017, Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes, Arch. Microbiol., 199, 1335, 10.1007/s00203-017-1409-7 Ritchie, 1996, Current statistical methods for estimating the Km and Vmax of Michaelis-Menten kinetics, Biochem. Educ., 24, 16, 10.1016/S0307-4412(96)00089-1 Mourad, 2012, Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis. Fed, Euro. Biol. Soc. Lett., 586, 1370, 10.1016/j.febslet.2012.03.058 Wensing, 2014, Identification and genetics of 6-thioguanine secreted by Erwinia species and its interference with the growth of other bacteria, Mol. Genet. Genom., 289, 215, 10.1007/s00438-013-0805-1 Coyne, 2013, Biosynthesis of the antimetabolite 6-thioguanine in Erwinia amylovora plays a key role in Fire Blight pathogenesis, Angew. Chem. Int. Ed., 52, 10564, 10.1002/anie.201305595 Pulawska, 2017, Comparative transcriptomic analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars – susceptible and resistant to fire blight, BMC Genom., 18, 868, 10.1186/s12864-017-4251-z