Amplification and expression of phenylalanine hydroxylase in mouse erythroleukemia cells
Tóm tắt
A cell line, CY-1, was selected in tyrosine free (tyr−) medium after fusion of mouse erythroleukemia (MEL) cells with mitomycin C-treated rat hepatoma cells. MEL cells do not express the enzyme phenylalanine hydroxylase (PH) and are unable to grow in tyr− medium, whereas the rat hepatoma cells constitutively express PH and are able to grow in tyr− medium. CY-1 cells resemble MEL cells morphologically, karyotypically, and in being inducible for hemoglobin synthesis. In contrast to MEL cells, CY-1 expresses PH and is therefore able to grow in tyr− medium. Using a rat cDNA probe for thePH gene, Southern blot analyses were carried out on DNA isolated from CY-1 and parental cells. CY-1 showed the characteristic mousePH gene pattern but the gene copy number was amplified four− to eightfold compared to parental MEL cells.
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