Amphiphilic/Bipolar Metallocorroles That Catalyze the Decomposition of Reactive Oxygen and Nitrogen Species, Rescue Lipoproteins from Oxidative Damage, and Attenuate Atherosclerosis in Mice
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1995, Oxidative Stress, Lipoproteins and Cardiovascular Dysfunction
Oxidation of small molecules: all reactions were performed with no additive or with1‐Feor1‐Mnin a phosphate buffer solution (pH 7.4). a) Formaldehyde from DMSO (160 μMcatalyst): DMSO (140 mM) was reacted with peroxynitrite (1.25 mM); b) malondialdehyde from deoxyribose (1 μMcatalyst): deoxyribose (10 mM) was reacted with peroxynitrite (390 μM); c) nitration of fluorescein (5 μMcatalyst): fluorescein (5 μM) was reacted with peroxynitrite (25 μM); d) nitration ofL‐tyrosine (50 μMcatalyst):L‐tyrosine (80 μM) was reacted with peroxynitrite (220 μM); e) copper‐induced oxidation of DMSO (50 μMcatalyst): CuSO4and phenanthroline (8 μMeach) were added to DMSO (140 mM) followed by sodium ascorbate (500 μM) or glutathione (1 mM).
TheKdfor the HDL–corrole conjugates may be estimated as much lower than 10−9 M−1from the relative concentrations of HDL and albumin in serum and the previously determinedKdof serum albumin–corrole conjugates (reference [10c]).
Oxidation of LDL: LDL (100 mg protein L−1) in phosphate‐buffered saline was incubated for 30 min without any additive or with various concentrations of1‐Mn 1‐Fe or1‐Ga. a) By peroxynitrite: oxidation was induced by addition of SIN‐1 (250 μM) and conjugated dienes were continuously monitored for 4 h at 37 °C; b) by copper sulfate: a freshly prepared CuSO4solution (5 μM) was added and the mixture was incubated at 37 °C. Oxidation was determined by measuring TBARS conjugated dienes and lipid peroxides by previously described methods (reference [23]).