Ameliorative Effect of Boerhavia Diffusa in Adenine-induced CKD Rat and Association of Aquaporin Transcript
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Tập 91 - Trang 789-797 - 2021
Tóm tắt
The membrane-associated water transport proteins called aquaporins are quite significant in water reabsorption from renal tubular fluid. The present study was performed to analyze the expression level of AQP isoforms in normal renal tissue, their alteration in the development of chronic kidney disease in rat induced by the administration of 0.75% adenine accompanied by their treatment with the ethanolic extract of Boerhavia diffusa. The qPCR analysis demonstrated an upregulation of certain aquaporin genes like aqp3 and aqp4 and a downregulation of other genes like aqp1 and aqp7. Furthermore, the treatment with the ethanolic extract of B. diffusa was pivotal in restoring the expression levels of these aquaporins to normal, i.e., upregulating the downregulated aquaporins and vice versa, thereby exemplifying its renoprotective nature. The serum urea and creatinine concentration were found to elevate in the infected group of rats accompanied by their lowering in the treated group. The analysis involving the determination of body weight and the urinary ACR manifested elevated values in the diseased group followed by their decline in the treated group. Histopathological study revealed clear signs of acute necrosis with tubular distention caused by the administration of adenine, while the treatment of rats with B. diffusa extract brought improvement in histologic appearance with decreased necrotic areas. Our findings implicate the probable involvement of certain aquaporins in adenine-induced chronic kidney disease and also the curative property of B. diffusa in reversing the condition back to normal as reflected by the functional, biochemical and histopathological parameters.
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