Alternative Methods for Measuring Inorganic, Organic, and Total Dissolved Nitrogen in Soil

Soil Science Society of America Journal - Tập 74 Số 3 - Trang 1018-1027 - 2010
Rebecca Hood‐Nowotny1, Nina Hinko‐Najera2, Erich Inselsbacher3, Petra Lachouani1,3,2, Wolfgang Wanek1
1Dep. of Chemical Ecology and Ecosystem Research Univ. of Vienna Althanstrasse 14 1090 Vienna Austria
2Dep. of Forest and Ecosystem Science Univ. of Melbourne Water St. Creswick VIC 3363 Australia
3Dep. of Forest Ecology and Management Swedish Univ. of Agricultural Sciences SE‐901, 83 Umeå Sweden

Tóm tắt

There are numerous methods for measuring inorganic, dissolved organic, and microbial N in soils, although many of these are complex or require expensive equipment. We have modified methods for the measurement of NH4+, NO3−, total dissolved N (TDN), and soil microbial biomass N (SMBN) in soils. The methods are based on a microtiter plate format and are rapid and simple to perform. Ammonium is quantified by a colorimetric method based on the Berthelot reaction. Total dissolved N and SMBN (by CH3Cl fumigation‐extraction) are quantified as NO3− after alkaline persulfate oxidation. Nitrate is estimated directly or after persulfate oxidation by reduction of NO3− to NO2− by VCl3 and subsequent colorimetric determination of NO2− by acidic Griess reaction. The new suite of methods was compared with conventional methods such as high‐performance anion‐exchange chromatography for NO3− and high‐temperature catalytic oxidation for TDN. Our methods produced comparable detection limits, linearities, and precisions compared with the conventional methods. Limits of quantification were 7 μg NH4+–N L−1, 55 μg NO3−–N L−1, and 0.275 mg TDN L−1 The accuracy of the proposed methods was excellent, with recoveries of added NH4+, NO3−, and glycine ranging between 96 and 99%. Linearities of the respective calibrations were high (R2 > 0.99), and precisions for NH4+ (CV = 2.1%), NO3− (CV = 3.5%), and TDN (CV = 3.9%) were comparable to the reference methods. The simplicity, rapidity, and low cost of the proposed methods therefore allow an expansion of the scope and range of N cycle studies where sophisticated instrumentation is not available.

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Tài liệu tham khảo

10.1021/es00036a015

10.1016/0038-0717(88)90134-4

10.1016/0038-0717(89)90069-2

10.1016/S0304-4203(99)00103-6

10.1016/0038-0717(85)90143-9

10.1016/0038-0717(85)90144-0

10.2136/sssaj1993.03615995005700040021x

10.1016/j.apsoil.2008.02.006

10.1016/j.jfca.2006.12.003

10.2134/jeq2001.2206

10.2136/sssaj2004.6690

10.1016/0038-0717(96)00006-5

Greenfield L.G., 1995, Release of microbial cell N during chloroform fumigation, Soil Biol. Biochem., 27, 1235, 10.1016/0038-0717(95)00023-8

10.1002/(SICI)1522-2624(200002)163:1<81::AID-JPLN81>3.0.CO;2-1

10.1016/S0076-6879(94)33028-X

10.1034/j.1399-3054.2000.100209.x

Inselsbacher E., 2009, Short‐term competition between crop plants and soil microbes for inorganic N fertilizer, Soil Biol. Biochem., 42, 360, 10.1016/j.soilbio.2009.11.019

IUSS Working Group WRB, 2006, A framework for international classification, correlation and communication

10.1016/j.soilbio.2003.10.002

10.1016/j.soilbio.2004.08.008

10.1016/j.soilbio.2005.08.012

10.1007/BF00257924

10.1006/niox.1997.0116

10.1016/0304-4203(94)00049-J

10.1006/niox.2000.0319

10.1016/S0038-0717(03)00014-2

10.1007/s003740050018

10.1016/0304-4203(93)90108-Z

10.2136/sssaj1998.03615995006200020026x

10.1080/03067310600739632

10.1016/j.marchem.2003.07.003

10.1080/00103629509369298

10.2136/sssaj1995.03615995005900030044x

Taylor J.K., 1987, Quality assurance of chemical measurements

10.2134/jeq2008.0277

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Thông tin xuất bản

Soil Science Society of America Journal

Tập 74 Số 3

1018-1027

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