Altered expression of thin filament‐associated proteins in hypertrophied urinary bladder smooth muscle

Neurourology and Urodynamics - Tập 25 Số 1 - Trang 78-88 - 2006
Anita Mannikarottu1,2, Michael E. DiSanto1, Stephen A. Zderic3, Alan J. Wein1, Samuel Chacko4,1
1Division of Urology, University of Pennsylvania, Philadelphia, Pennsylvania
2Dr. Mannikarottu is a trainee in the Urology Training grant T32-DK07708.
3Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
4Division of Pathobiology, University of Pennsylvania, Philadelphia, Pennsylvania

Tóm tắt

AbstractAimsObstruction of the urinary bladder outlet induces detrusor smooth muscle (DSM) hypertrophy. The goal of this study was to determine whether the composition of thin filament‐associated proteins, known to play important roles in cytoskeletal structure and/or the regulation of contraction, is altered in DSM during hypertrophy.MethodsDSM hypertrophy was induced in male rabbits by partial ligation of the urethra. Sham‐operated rabbits served as a control. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) and real‐time PCR revealed a significant increase in the expression of mRNAs for basic (h1) calponin (CaP), and α‐isoform of tropomyosin (Tm) in hypertrophied DSM compared to controls. Western blotting and two‐dimensional (2‐D) gel electrophoresis showed enhanced expression of these proteins and also a significant increase in the expression of β‐non muscle and γ‐smooth muscle actin in the DSM from obstructed bladders, while α‐actin remained constant.ResultsEnhanced expression of these proteins in the DSM from obstructed bladders was confirmed by immunofluorescence microscopy. Double immunostaining with Cap/Tm and α/β‐actin‐specific antibodies showed co‐localization of these proteins in myocytes. Colocalization of smooth muscle specific myosin and CaP to cytoplasmic filaments in cells dissociated from the hypertrophied DSM indicated that these cells are differentiated smooth muscle cells.ConclusionsThe change in the isoforms of actin, Cap, and Tm may be part of the molecular mechanism for bladder compensation in increased urethral resistance. Neurourol. Urodynam. © 2005 Wiley‐Liss, Inc.

Từ khóa


Tài liệu tham khảo

10.1093/oxfordjournals.jbchem.a123289

10.1146/annurev.bi.49.070180.004421

10.1016/S0021-9258(17)34113-3

10.1016/0167-4889(93)90146-G

10.1021/bi960996j

10.1242/jcs.115.10.2021

10.1016/S0022-5347(05)66585-8

Buttyan R, 1997, Animal models of bladder outlet obstruction and molecular insights into the basis for the development of bladder dysfunction, Eur Urol, 32, 32

10.1139/y94-204

Chacko S, 1990, Cooperativity of actin‐activated ATPase of gizzard heavy meromyosin in the presence of gizzard tropomyosin, J Biol Chem, 265, 2105, 10.1016/S0021-9258(19)39946-6

10.1007/978-1-4615-4737-2_11

10.1152/ajpcell.00513.2002

10.1083/jcb.111.6.2463

10.1016/S0094-0143(05)70304-9

Fatigati V, 1984, Actin and tropomyosin variants in smooth muscles. Dependence on tissue type, J Biol Chem, 259, 14383, 10.1016/S0021-9258(17)42610-X

10.1006/bbrc.1996.0051

10.1016/0014-5793(90)81353-P

Hartshorne DJ, 1987, Physiology of the gastrointestinal tract, 423

10.1016/0006-291X(91)90455-G

10.1007/BF00925947

10.1016/S0022-5347(17)47183-7

10.1152/ajpcell.1991.260.5.C1085

10.1093/oxfordjournals.jbchem.a021428

10.1016/S0022-5347(17)38477-X

10.1016/0378-1119(93)90435-6

10.1161/01.RES.75.1.33

North AJ, 1994, Calponin is localised in both the contractile apparatus and the cytoskeleton of smooth muscle cells, J Cell Sci, 107, 437, 10.1242/jcs.107.3.437

North AJ, 1994, Actin isoform compartments in chicken gizzard smooth muscle cells, J Cell Sci, 107, 445, 10.1242/jcs.107.3.445

10.1152/ajpcell.1994.267.5.C1262

10.1083/jcb.102.1.210

10.1016/S0022-5347(05)67242-4

10.1016/0014-5793(93)80909-E

Takahashi K, 1991, Molecular cloning and sequence analysis of smooth muscle calponin [published erratum appears in J Biol Chem 1992 Dec 25; 267(36):26198], J Biol Chem, 266, 13284

10.1016/S0006-291X(86)80328-X

10.1161/01.HYP.11.6.620

10.1111/j.1464-410X.2003.04719.x

10.1042/bj3060211

Uvelius B, 1984, Smooth muscle cell hypertrophy and hyperplasia in the rat detrusor after short‐term intravesical outflow obstruction, J Urol, 131, 173, 10.1016/S0022-5347(17)50253-0

10.1111/j.1432-0436.1979.tb01021.x

10.1152/ajpcell.1993.265.5.C1371

Wang ZE, 1995, Expression of smooth muscle myosin isoforms in urinary bladder smooth muscle during hypertrophy and regression, Lab Invest, 73, 244

Wein AJ, 1995, Assessing treatment results in benign prostatic hyperplasia, Urol Clin North Am, 22, 345, 10.1016/S0094-0143(21)00670-4

10.1016/S0021-9258(19)38792-7

10.1016/S0022-5347(01)65758-6

Zderic SA, 2002, Adult and pediatric urology, 1061

10.1016/S0002-9440(10)63149-5

Thông tin
Thông tin xuất bản

Neurourology and Urodynamics

Tập 25 Số 1

78-88

Thông tin tác giả