Alterations in the peripheral blood B cell subpopulations of multidrug-resistant tuberculosis patients

Clinical and Experimental Medicine - Tập 14 - Trang 423-429 - 2013
Mónica T. Abreu1, Helena Carvalheiro1, Tiago Rodrigues-Sousa1, António Domingos2, António Segorbe-Luis3,4, Paulo Rodrigues-Santos1,5, M. Margarida Souto-Carneiro1
1Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal
2Department of Pneumology, Hospital de Torres Vedras, Centro Hospitalar Centro Tejo, Torres Vedras, Portugal
3Faculty of Medicine, University of Coimbra, Coimbra, Portugal
4Department of Immunoallergology, Hospitais da Universidade de Coimbra, Coimbra, Portugal
5Immunology Institute, Faculty of Medicine, University of Coimbra, Coimbra, Portugal

Tóm tắt

The function of B cells in the immune response against Mycobacterium tuberculosis (Mtb) is still regarded as secondary, although major findings in mouse models of tuberculosis (TB) support their participation as regulators and antibody producers. However, studies in cohorts of TB or multidrug-resistant TB (MDR-TB) patients have failed to clearly identify changes in the circulating B cell pool. Therefore, in the present study we aimed at identifying alterations in the different B cell subpopulations in peripheral blood samples of HIV-negative pulmonary MDR-TB patients when compared to healthy donors. The data show, for the first time, that MDR-TB patients, similarly to what has been observed in other chronic inflammatory diseases, have a much lower frequency of peripheral blood unswitched IgD+CD27+ memory B cells. Equally novel are the findings that in MDR-TB patients there is a reduction in the circulating plasma cell pool and that in MDR-TB there is an increased frequency of circulating type 1 transitional IgD+CD38++, CD69+ and TLR9+ B cells. These results document disease-related shifts in peripheral blood B cell subsets in MDR-TB and suggest that such changes should be taken into account when designing new strategies to boost the cellular and humoral immune response against Mtb.

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