Alleviation of browning in oak explants by chemical pretreatments
Tóm tắt
Meristems from 25–90-year-old oak (Quercus robur L. andQ. petraea Matt.) trees and seed embryos were pretreated with polyvinyl pyrrolidone, ascorbic acid, cysteine and citric acid solutions. Tissues were cultured mostly on a WPM medium supplemented with different combinations and concentrations of growth regulators. All the different pretreatments showed a positive effect against the otherwise very rapid and harmful browning of the explants but ascorbic acid (100 mg dm−3) proved to be the most effective. Shooting was induced from seed embryos and meristems originating from adult trees. Rooted plantlets were obtained from explants of seed embryos.
Tài liệu tham khảo
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