Aging Reversibility: from Thymus Graft to Vegetable Extract Treatment – Application to Cure an Age-associated Pathology
Tóm tắt
Neonatal thymus graft and thymus calf extract (TME) in vivo treatment exert similar corrective actions on different mouse age-related alterations. The aim of the present paper is to investigate whether a vegetal extract, wheat sprout extract (WESPRE), could mimic the thymus action on recovering age-related alterations and if this extract can cure an age-associated pathology, the cataract in dogs. Present experiments were carried out by using WESPRE and TME in vivo in old mice to check their ability to recover the altered DNA synthesis in hepatocyte primary cultures. Old mice treated with WESPRE and TME showed a recovery of hepatocyte DNA synthesis levels when compared with the old untreated ones. The increase of DNA and protein contents observed in aged animals is reduced by WESPRE treatments to levels observed in young mice hepatocytes. We measured also WESPRE phosphorylation activity by endogenous kinase: it was from 10 to 40 times higher with respect to wheat seeds. Old dogs were orally treated for a month and the lens opacity analysed before and after the treatment. Results showed a reduction from 25 to 40% of lens opacity. The efficacy of wheat sprouts in the recovery of age-related alterations and in treating age-associated pathologies could be due to the contemporary presence of small regulatory acid peptides, a remarkable level of highly energetic phosphoric radicals and antioxidant molecules, peculiarities that may be, to some extent, related to the aging process regulation.
Tài liệu tham khảo
D Amici GB Rossi L CioS GP Matarese A Dolei L Guglielmi (1983) ArticleTitleLow-molecular-weight peptide inhibiths RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells Prot Natl Acad Sci USA 74 3869–3873
RE Anderson RL Kretzer LM Rapp (1994) ArticleTitleFree radicals and ocular disease Adv Exp Med Biol 366 73–86 Occurrence Handle1:CAS:528:DyaK2MXpsl2gu78%3D Occurrence Handle7771292
HK Biesalski (2002) ArticleTitleFree radical theory of aging Opin Curr Clin Nutr Metab Care 5 IssueID1 5–10 Occurrence Handle1:CAS:528:DC%2BD38XltlGhsQ%3D%3D
A Basso L Piantanelli G Rossolini GS Roth (1998) ArticleTitleReduced DNA synthesis in primary cultures of hepatocytes from old mice is restored by thymus graft J Gerontol 53A B111–B116 Occurrence Handle1:CAS:528:DyaK1cXit1ars7o%3D
A Basso G Rossolini G Gianfranceschi D Amici L Piantanelli (1993) Reversibility of age-related alterations of b-adrenergic responsiveness by thymus extract and thymus graft E Beregi IA Gergely K Rajczi (Eds) Recent Advances in Aging Science Monduzzi Editore S.p.A Bologna 233–236
K Burton (1956) ArticleTitleA study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid Biochem J 62 315–323 Occurrence Handle1:CAS:528:DyaG28XktVyktQ%3D%3D Occurrence Handle13293190
I Calzuola V Marsili GL Gianfranceschi (2004) ArticleTitleSynthesis of antioxidants in wheat sprouts J Agric Food Chem 52 IssueID16 5201–5206 Occurrence Handle10.1021/jf0307752 Occurrence Handle1:CAS:528:DC%2BD2cXls1Kqs7Y%3D Occurrence Handle15291497
M Dardenne W Savino (1990) ArticleTitleNeuroendocrine control of the thymic epithelium: modulation of thymic endocrine function, cytokeratin expression and cell proliferation by hormones and neuropeptides Prog Neuroendocr Immunol 3 18–25
W Droge (2002) ArticleTitleFree radicals in the physiological control of cell function Physiol Rev 82 IssueID1 47–95 Occurrence Handle1:CAS:528:DC%2BD38XovFSisw%3D%3D Occurrence Handle11773609
N Fabris L Piantanelli (1982) Thymus neuroendocrine interaction during development and aging R Adelman G Roth (Eds) Hormones and Ageing CRC Press Palm Beach 186–196
G Falcioni D Fedeli L Tiano I Calzuola L Mancinelli V Marsili GL Gianfranceschi (2002) ArticleTitleAntioxidant activity of wheap sprouts extracts “in vitro”: inhibition of DNA oxidative damage J Food Sci 67 2918–2920 Occurrence Handle1:CAS:528:DC%2BD38XptVent7g%3D
R Fautz B Husein E Efstathiou C Hechenberger-Freudl (1993) ArticleTitleAssessment of the relation between the initial viability and the attachment of freshly isolated rat hepatocytes used for the in vitro/in vitro DNA repair assay (UDS) Mutat Res 291 21–27 Occurrence Handle1:CAS:528:DyaK3sXhsVarur8%3D Occurrence Handle7678911
GL Gianfranceschi D Amici L Guglielmi (1976a) ArticleTitleRestriction of template capacity of rat liver chromatin by a non-histone peptide from calf thymus Nature 262 IssueID5569 622–623 Occurrence Handle10.1038/262622a0 Occurrence Handle1:CAS:528:DyaE2sXht1Sgsg%3D%3D
GL Gianfranceschi D Amici L Guglielmi (1976b) ArticleTitleStabilization of double-stranded DNA molecule by non-histone peptidic effector from calf thymus Molecular Biology Reports 3 55–64 Occurrence Handle10.1007/BF00357209 Occurrence Handle1:CAS:528:DyaE2sXosFSgtQ%3D%3D
GL Gianfranceschi D Amici L Guglielmi (1975) ArticleTitleEvidence for the presence in calf thymus of a peptidic factor controlling DNA transcription in vitro Biochim Biophys Acta 414 9–19 Occurrence Handle1:CAS:528:DyaE28Xhs12l Occurrence Handle1191703
GL Gianfranceschi D Amici L Guglielmi (1974) ArticleTitleAn aqueous thymus extract modifies DNA-proteins interactions in the liver of old rats Spectrophotometrical data Experientia 30 1049–1050 Occurrence Handle1:CAS:528:DyaE2cXlsV2htrk%3D
TR Golden (2002) ArticleTitleOxidative stress and aging: beyond correlation Agin Cell 1 IssueID2 117–123 Occurrence Handle1:CAS:528:DC%2BD3sXktlSqt7s%3D
A Goldstein M Badamchian (2004) ArticleTitleThymosins: chemistry and biological properties in health and disease Expert Opinion on Biological Therapy 4 559–573 Occurrence Handle10.1517/14712598.4.4.559 Occurrence Handle1:CAS:528:DC%2BD2cXis1Oqtrk%3D Occurrence Handle15102605
E Hannappel T Huff (2003) ArticleTitleThe thymosin. Prothymosin alpha, parathymosin, and beta-thymosins structure and function Vitam Horm 66 257–269 Occurrence Handle1:CAS:528:DC%2BD3sXmvFOhtL0%3D Occurrence Handle12852257
A Ishigami TD Reed GS Roth (1993) ArticleTitleEffect of aging on EGF stimulated DNA synthesis and EGF receptor levels in primary cultured rat hepatocytes Biochem Biophys Res Commun 196 181–186 Occurrence Handle10.1006/bbrc.1993.2232 Occurrence Handle1:CAS:528:DyaK3sXms1egsb4%3D Occurrence Handle8216291
OH Lowry NJ Rosebrough AL Farr RJ Randall (1951) ArticleTitleProtein measurement with the Folin phenol reagent J Biol Chem 193 265–275 Occurrence Handle1:CAS:528:DyaG38XhsVyrsw%3D%3D Occurrence Handle14907713
L Mancinelli F Chillemi E Cardellini V Marsili F Giavarini L Angelis ParticleDe G Lugaro GL Gianfranceschi (1999) ArticleTitleMolecular models of acid petides from pea bud chromatin and seminal plasmaDivalent cation-mediated interaction with DNA Biol Chem 380 31–40 Occurrence Handle10.1515/BC.1999.004 Occurrence Handle1:CAS:528:DyaK1MXhtlOgsrw%3D Occurrence Handle10064134
L Mancinelli V Marsili GL Gianfranceschi (1998) ArticleTitlePresence of biologically active substances in the wheat bud Gastroenterol Int 11 50–51
L Mancinelli E Castigli P Qualadrucci GL Gianfranceschi M Bramucci A Miano D Amici (1992) ArticleTitleSmall acidic peptides from wheat germ chromatin. I. Isolation and biochemical characterization Physiol Chem Phys Med NMR 24 97–107 Occurrence Handle1:CAS:528:DyaK38XmtVygurc%3D Occurrence Handle1508994
V Marsili I Calzuola GL Gianfranceschi (2004) ArticleTitleNutritional relevance of wheat sprouts containing high levels of organic phosphates and antioxidant compounds J Clin Gastroenterol 38 IssueID6 Suppl S123–S126 Occurrence Handle1:CAS:528:DC%2BD2cXlt1Sks70%3D Occurrence Handle15220677
S Melov (2002) ArticleTitleTherapeutics against mitochondrial oxidative stress in animal models of aging Ann NY Acad Sci 959 330–340 Occurrence Handle1:CAS:528:DC%2BD38XktFWqu7o%3D Occurrence Handle11976207
J Miquel (2002) ArticleTitleCan antioxidant diet supplementation protect against age-related mitochondrial damage? Ann NY Acad Sci 959 508–516 Occurrence Handle1:CAS:528:DC%2BD38XktFajsrc%3D Occurrence Handle11976223
Y Niwa K Tominaga K Yoshida (1998) ArticleTitleSuccessful treatment of severe atopic dermatitis-complicated cataract and male infertility with a natural product antioxidant Int J Tissue React 20 63–69 Occurrence Handle1:CAS:528:DyaK1cXjvVCis7k%3D Occurrence Handle9638503
H Orham S Marol IF Hepsen G Sahin (1999) ArticleTitleEffects of some probable antioxidants on selenite-induced cataract formation and oxidative stress-related parameters in rats Toxicology 139 219–232
B Peryt T Szymczyk P Lesca (1992) ArticleTitleMechanism of antimutagenicity of wheat sprout extracts Mutat Res 269 201–215 Occurrence Handle1:CAS:528:DyaK3sXit1Kk Occurrence Handle1383703
L Piantanelli G Rossolini C Viticchi A Basso A Zaia (1991) ArticleTitleFunctional interrelationships in aging processes: alterations and reversibility Arch Gerontol Geriatr 12 291–301 Occurrence Handle10.1016/0167-4943(91)90035-O Occurrence Handle1:STN:280:DC%2BD2cvmtl2kuw%3D%3D Occurrence Handle15374455
L Piantanelli P Fattoretti C Viticchi (1980) ArticleTitleBeta-adrenoceptor changes in submandibular glands of old mice Mech Ageing Dev 14 155–164 Occurrence Handle10.1016/0047-6374(80)90114-1 Occurrence Handle1:CAS:528:DyaL3MXmt1Kktg%3D%3D Occurrence Handle6259456
L Piantanelli R Brogli P Bevilacqua N Fabris (1978) ArticleTitleAge dependence of Isoproterenol-induced DNA synthesis in submandibular glands of Balb/c mice Mech Ageing Dev 7 IssueID3 163–169 Occurrence Handle1:CAS:528:DyaE1cXhs1ChtbY%3D Occurrence Handle621968
C Pieri C Giuli C Bertoni-Freddari L Piantanelli IZ Nagy (1982) ArticleTitleThymic control on the polyploidization of hepatocytes during aging Experientia 38 680–681 Occurrence Handle10.1007/BF01964092 Occurrence Handle1:CAS:528:DyaL38XksFSiurg%3D Occurrence Handle7106236
C Pieri C Giuli M Moro ParticleDel L Piantanelli (1980) ArticleTitleElectron-microscopic morphometric analysis of mouse liver. II. Effect of ageing and thymus transplantation in old animals Mech Ageing Dev 13 275–283 Occurrence Handle10.1016/0047-6374(80)90040-8 Occurrence Handle1:STN:280:DyaL3M%2FivFGjtg%3D%3D Occurrence Handle7421302
G Rossolini A Basso L Piantanelli R Tacconi D Amici GL Gianfranceschi (1992) ArticleTitleNeuroendocrine thymus and b-adrenergic responsiveness in aging mice Arch Gerontol Geriatr 3 311–318 Occurrence Handle1:CAS:528:DyaK3sXhsVSgtb4%3D
G Rossolini C Viticchi A Basso A Zaia L Piantanelli (1991) ArticleTitleThymus-induced recovery of age-related decrease of brain cortex a- and b-adrenoceptors Int J Neurosci 59 143–150 Occurrence Handle1:STN:280:DyaK387ivFSlug%3D%3D Occurrence Handle1663493 Occurrence Handle10.3109/00207459108985457
PO Seglen (1976) ArticleTitlePreparation of isolated rat liver cells Methods Cell Biol 13 29–83 Occurrence Handle1:CAS:528:DyaE28XkslCgtbY%3D Occurrence Handle177845
S Takai T Nakamura N Komi A Ichiara (1988) ArticleTitleMechanisms of stimulation of DNA synthesis induced by epinephrine in primary cultures of adult rat hepatocytes Biochem J 103 848–852 Occurrence Handle1:CAS:528:DyaL1cXktVWhtLg%3D
A Taylor PF Jacques EM Epstein (1995) ArticleTitleRelation among aging, antioxidant status and cataract Am J Clin Nutr 62 IssueID6 Suppl 1439S–1447S Occurrence Handle1:STN:280:DyaK28%2FpsVOjsA%3D%3D Occurrence Handle7495245
B Tudek B Peryt J Miloszewska T Szymczyk M Przybyszewska P Janik (1998) ArticleTitleThe effect of wheat sprout extract on benzo(a)pyrene and 7,2-dimethyl- benz(a)anthracene activity Neoplasma 35 515–523
JC Pols ParticleVan der (1999) ArticleTitleA possible role for vitamin C in age-related cataract Proc Nutr Soc 58 IssueID2 295–301 Occurrence Handle10466170
C Viticchi S Gentile L Piantanelli (1989) ArticleTitleAgeing and thymus-induced differential regulation of beta−1 and beta−2 adrenoceptors of mouse brain cortex Arch Gerontol Geriatr 8 13–20 Occurrence Handle10.1016/0167-4943(89)90065-4 Occurrence Handle1:CAS:528:DyaL1MXhtVylurk%3D Occurrence Handle2540723
A Zaia Piantanelli (1997) ArticleTitleInsulin receptors in mouse brain: age-related modifications are corrected by thymus graft Mech Ageing Dev 98 37–46 Occurrence Handle10.1016/S0047-6374(97)00066-3 Occurrence Handle1:CAS:528:DyaK2sXltVWnt7w%3D Occurrence Handle9255756