Age-associated changes in the electrophoretic pattern of the high molecular weight non-histone proteins from mouse liver, hepatoma and spleen chromatin
Tóm tắt
The water-soluble fractions of non-histone chromatin (NHC) proteins were isolated from 0.35M NaCl extracts of liver and spleen chromatin of young and old CBA mice and hepatomas of old mice. SDS-disc electrophoretic patterns of proteins with higher molecular weight were compared. Tissue specificity and age changes of the patterns have been found; however, in spleen chromatin the differences of NHC proteins associated with aging were insignificant. Age-associated changes of NHC proteins in liver (increase of number of minor protein fractions) are not found in chromatin from hepatomas which indicates that the changes of the pattern of NHC proteins in aged liver cells are probably reversible.
Tài liệu tham khảo
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