Agarose and Its Derivatives as Supports for Enzyme Immobilization

Springer Science and Business Media LLC - Tập 21 Số 11 - Trang 1577
Paolo Zucca1, Roberto Fernández‐Lafuente2,3, Enrico Sanjust1
1Dipartimento di Scienze Biomediche, Università di Cagliari, 09042 Monserrato (CA), Italy
2C/Marie Curie 2, Campus UAM-CSIC, Madrid 28049, Spain
3Departamento de Biocatalisis, ICP-CSIC

Tóm tắt

Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and sharply hydrophilic. These features—that could be further improved by means of covalent cross-linking—render them particularly suitable for enzyme immobilization with a wide range of derivatization methods taking advantage of chemical modification of a fraction of the polymer hydroxyls. The main properties of the polymer are described here, followed by a review of cross-linking and derivatization methods. Some recent, innovative procedures to optimize the catalytic activity and operational stability of the obtained preparations are also described, together with multi-enzyme immobilized systems and the main guidelines to exploit their performances.

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