Affinity binding on argon plasma-immobilized biotinylated phospholipids

Springer Science and Business Media LLC - Tập 1 - Trang 261-277 - 1996
Mark E. Blaylock1, Allan S. Hoffman1
1Center for Bioengineering, University of Washington, Seattle

Tóm tắt

Dioleoylphosphatidylcholine (DOPC) and N-biotinyl-dioleoylphosphatidylethanolamine (bDOPE) were covalently bonded to polystyrene surfaces by exposure to argon radio frequency (RF) plasmas. Characterization by electron spectroscopy for chemical analysis (ESCA) for P-, N- and O-contents revealed net increases in oxygen species, but no significant changes in P/N ratios due to the different plasma treatments. Plasma-immobilized bDOPE was shown to bind avidin by affinity interactions. Nonspecific adsorption of avidin was comparable on plasma-immobilized DOPC and bDOPE-DOPC mixed-lipid coatings.

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