Aerobic and anaerobic methanotrophic communities in urban landscape wetland
Tóm tắt
Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) organisms can be important methane sinks in a wetland. However, the influences of the vegetation type on aerobic MOB and n-damo communities in wetland, especially in constructed wetland, remain poorly understood. The present study investigated the influences of the vegetation type on both aerobic MOB and n-damo organisms in a constructed urban landscape wetland. Sediments were collected from eight sites vegetated with different plant species. The abundance (1.19–3.27 × 107 pmoA gene copies per gram dry sediment), richness (Chao1 estimator = 16.3–81.5), diversity (Shannon index = 2.10–3.15), and structure of the sediment aerobic MOB community were found to vary considerably with sampling site. In contrast, n-damo community abundance (8.74 × 105–4.80 × 106 NC10 16S rRNA gene copies per gram dry sediment) changed slightly with the sampling site. The richness (Chao1 estimator = 1–11), diversity (Shannon index = 0–0.78), and structure of the NC10 16S rRNA gene-based n-damo community illustrated slight site-related changes, while the spatial changes of the pmoA gene-based n-damo community richness (Chao1 estimator = 1–8), diversity (Shannon index = 0–0.99), and structure were considerable. The vegetation type could have a profound impact on the wetland aerobic MOB community and had a stronger influence on the pmoA-based n-damo community than on the NC10 16S-based one in urban wetland. Moreover, the aerobic MOB community had greater abundance and higher richness and diversity than the n-damo community. Methylocystis (type II MOB) predominated in urban wetland, while no known type I MOB species was detected. In addition, the ratio of total organic carbon to total nitrogen (C/N) might be a determinant of sediment n-damo community diversity and aerobic MOB richness.
Tài liệu tham khảo
Chen J, Zhou ZC, JD G (2015) Complex community of nitrite dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes. Appl Microbiol Biotechnol 99:1463–1473
Chen XP, Ma H, Zheng Y, Liu JM, Liang X, He CQ (2017) Changes in methane emission and methanogenic and methanotrophic communities in restored wetland with introduction of Alnus trabeculosa. J Soils Sediments 17:181–189
Chowdhury TR, Dick RP (2013) Ecology of aerobic methanotrophs in controlling methane fluxes from wetlands. Appl Soil Ecol 65:8–22
Conrad R (2007) Microbial ecology of methanogens and methanotrophs. Adv Agron 96:1–63
Dai Y, Wu Z, Xie SG, Liu Y (2015) Methanotrophic community abundance and composition in plateau soils with different plant species and plantation ways. Appl Microbiol Biotechnol 99:9237–9244
Danilova OV, Belova SE, Gagarinova IV, Dedysh SN (2016) Microbial community composition and methanotroph diversity of a subarctic wetland in Russia. Microbiology 85:583–591
Deng YC, Cui XY, Luke C, Dumont MG (2013) Aerobic methanotroph diversity in Riganqiao peatlands on the Qinghai–Tibetan Plateau. Environ Microbiol Rep 5:566–574
Ettwig KF, van Alen T, van de Pas-Schoonen KT, Jetten MS, Strous M (2009) Enrichment and molecular detection of denitrifying methanotrophic bacteria of the NC10 phylum. Appl Environ Microbiol 75:3656–3662
Ettwig KF, Butler MK, Le Paslier D, Pelletier E, Mangenot S, Kuypers MMM, Schreiber F, Dutilh BE, Zedelius J, de Beer D, Gloerich J, Wessels HJCT, van Alen T, Luesken F, Wu ML, van de Pas-Schoonen KT, Op den Camp HJM, Janssen-Megens EM, Francoijs KJ, Stunnenberg H, Weissenbach J, Jetten MSM, Strous M (2010) Nitrite-driven anaerobic methane oxidation by oxygenic bacteria. Nature 464:543–548
Ho A, Luke C, Cao ZH, Frenzel P (2011) Ageing well: methane oxidation and methane oxidizing bacteria along a chronosequence of 2000 years. Environ Microbiol Rep 3:738–743
Hu BL, Shen LD, Lian X, Zhu Q, Liu S, Huang Q, He ZF, Geng S, Cheng DQ, Lou LP, XY X, Zheng P, He YF (2014) Evidence for nitrite-dependent anaerobic methane oxidation as a previously overlooked microbial methane sink in wetlands. Proc Natl Acad Sci U S A 111:4495–4500
Jiang N, Wang Y, Dong X (2010) Methanol as the primary methanogenic and acetogenic precursor in the cold Zoige wetland at Tibetan Plateau. Microb Ecol 60:206–213
Kolb S, Knief C, Stübner S, Conrad R (2003) Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays. Appl Environ Microbiol 69:2423–2429
Lee SH, Kang H (2016) The activity and community structure of total bacteria and denitrifying bacteria across soil depths and biological gradients in estuary ecosystem. Appl Microbiol Biotechnol 100:1999–2010
Liu Y, Zhang JX, Zhao L, Li YZ, Yang YY, Xie SG (2015) Aerobic and nitrite-dependent methane-oxidizing microorganisms in sediments of freshwater lakes on the Yunnan Plateau. Appl Microbiol Biotechnol 99:2371–2381
Long Y, Yi H, Chen SL, Zhang ZK, Cui K, Bing YX, Zhuo QF, Li BX, Xie SG, Guo QW (2016) Influences of plant type on bacterial and archaeal communities in constructed wetland treating polluted river water. Environ Sci Pollut Res 23:19570–19579
Long Y, Jiang XJ, Guo QW, Li BX, Xie SG (2017a) Sediment nitrite dependent methane-oxidizing microorganisms temporally and spatially shift in the Dongjiang River. Appl Microbiol Biotechnol 101:401–410
Long Y, Guo QW, Li NN, Li BX, Tong TL, Xie SG (2017b) Spatial change of reservoir nitrite-dependent methane-oxidizing microorganisms. Ann Microbiol 67:165–174
Narihiro T, Hori T, Nagata O, Hoshino T, Yumoto I, Kamagata Y (2011) The impact of aridification and vegetation type on changes in the community structure of methane-cycling microorganisms in Japanese wetland soils. Biosci Biotechnol Biochem 75:1727–1734
Rietl AJ, Overlander ME, Nyman AJ, Jackson CR (2016) Microbial community composition and extracellular enzyme activities associated with Juncus roemerianus and Spartina alterniflora vegetated sediments in Louisiana saltmarshes. Microb Ecol 71:290–303
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing MOTHUR: open-source, platform-independent, community supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541
Shen LD, Huang Q, He ZF, Lian X, Liu S, He YF, Lou LP, XY X, Zheng P, BL H (2015) Vertical distribution of nitrite-dependent anaerobic methane-oxidising bacteria in natural freshwater wetland soils. Appl Microbiol Biotechnol 99:349–357
Sow SLS, Khoo G, Chong LK, Smith TJ, Harrison PL, Ong HKA (2014) Molecular diversity of the ammonia-oxidizing bacteria community in disused tin-mining ponds located within Kampar, Perak, Malaysia. World J Microbiol Biotechnol 30:757–766
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Tian JQ, Chen H, Dong XZ, Wang YF (2012) Relationship between archaeal community structure and vegetation type in a fen on the Qinghai–Tibetan Plateau. Biol Fertil Soils 48:349–356
Wang Y, Zhu G, Harhangi HR, Zhu B, Jetten MSM, Yin C, Op den Camp HJM (2012) Co-occurrence and distribution of nitrite-dependent anaerobic ammonium and methane-oxidizing bacteria in a paddy soil. FEMS Microbiol Lett 336:79–88
Wang Y, Huang P, Ye F, Jiang Y, Song LY, Op den Camp HJM, Zhu GB, SJ W (2016) Nitrite-dependent anaerobic methane oxidizing bacteria along the water level fluctuation zone of the Three Gorges Reservoir. Appl Microbiol Biotechnol 100:1977–1986
Yang MX, Guo QW, Tong TL, Li NN, Xie SG, Long Y (2017) Vegetation type and layer depth influence nitrite-dependent methane-oxidizing bacteria in constructed wetland. Arch Microbiol 199:505–511
Yang YY, Shan JW, Zhang JX, Zhang XL, Xie SG, Liu Y (2014) Ammonia- and methane-oxidizing microorganisms in high-altitude wetland sediments and adjacent agricultural soils. Appl Microbiol Biotechnol 98:10197–10209
Yang YY, Zhao Q, Cui YH, Wang YL, Xie SG, Liu Y (2016) Spatio-temporal variation of sediment methanotrophic microorganisms in a large eutrophic lake. Microb Ecol 71:9–17
Yi XH, Jing DD, Wan JQ, Ma YW, Wang Y (2016) Temporal and spatial variations of contaminant removal, enzyme activities, and microbial community structure in a pilot horizontal subsurface flow constructed wetland purifying industrial runoff. Environ Sci Pollut Res 23:8565–8576
Yun JL, Zhuang GQ, Ma AZ, Guo HG, Wang YF, Zhang HX (2012) Community structure, abundance, and activity of methanotrophs in the Zoige wetland of the Tibetan Plateau. Microb Ecol 63:835–843
Yun J, Yu Z, Li K, Zhang H (2013) Diversity, abundance and vertical distribution of methane-oxidizing bacteria (methanotrophs) in the sediments of the Xianghai wetland, Songnen Plain, northeast China. J Soils Sediments 13:242–252
Yun JL, YW J, Deng YC, Zhang HX (2014) Bacterial community structure in two permafrost wetlands on the Tibetan Plateau and Sanjiang Plain, China. Microb Ecol 68:360–369
Yun JL, Zhang HX, Deng YC, Wang YF (2015) Aerobic methanotroph diversity in Sanjiang wetland, northeast China. Microb Ecol 69:567–576
Zhu GB, Zhou LL, Wang Y, Wang SY, Guo JH, Long XE, Sun XB, Jiang B, Hou QY, Jetten MSM, Yin CQ (2015) Biogeographical distribution of denitrifying anaerobic methane oxidizing bacteria in Chinese wetland ecosystems. Environ Microbiol Rep 7:128–138