Adventitious in vitro plantlet formation from immature floral stems of Crinum macowanii
Tóm tắt
Adventitious shoots and plantlets were regenerated in vitro from floral stem explants of Crinum macowanii (Bak.) (bush lily). The length (age) of the floral stem as well as the orientation and position of the explant disc in the floral stem were the most important factors affecting shoot regeneration. The highest number of shoots were regenerated when immature floral stems of 70–100 mm were used as starting material, using the middle or basal parts of the stem, and orientating the discs with their proximal ends on the medium. Combinations of kinetin (4.65 μM) and either indoleacetic acid (0.57 μM) or naphthaleneacetic acid (0.54 μM), or a combination of benzyladenine (4.44 μM) and 2,4-dichlorophenoxyacetic acid (0.45 μM) resulted in the highest numbers of shoots being regenerated. Although a slight degree of callus formation was noticed on the cut-edges of the discs, shoot formation did not occur via callus, but directly from the floral stem epidermis. Unrooted shoots were rooted on MS-medium containing 0.17 M sucrose.
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Tài liệu tham khảo
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