Adult Rat Dorsal Root Ganglion Neurons Extend Neurites on Predegenerated But Not on Normal Peripheral Nerves In Vitro

European Journal of Neuroscience - Tập 4 Số 3 - Trang 193-200 - 1992
Kuldip S. Bedi1,2, Janet Winter3, Martin Berry2, James Cohen2
1Department of Anatomy, University of Queensland, St. Lucia 4072, Australia
2Division of Anatomy and Cell Biology, UMDS–Guy's Campus, London SE1 9RT, UK
3Sandoz Institute for Medical Research, 5 Gower Place, London WC1E 6BT UK

Tóm tắt

AbstractThe abilities of embryonic and adult rat sensory neurons to regenerate were compared when cultured on cryostat sections of normal and lesioned sciatic nerve tissues. Differences in neurite growth, visualized by GAP‐43 immunolabelling, were most pronounced on substrata consisting of longitudinal sections of normal versus predegenerated sciatic nerve. Adult dorsal root ganglion (DRG) neurons grew only on the lesioned nerves. Neurites extended along these sections in a characteristically longitudinal orientation, and this growth was not dependent on nerve growth factor. Embryonic DRG neurons extended neurites on sections from both types of nerves. These results highlight important differences in the regenerative abilities of embryonic and adult DRG neurons when grown on physiologically appropriate substrata.

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European Journal of Neuroscience

Tập 4 Số 3

193-200

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