Adiponectin protects rat hippocampal neurons against excitotoxicity

Springer Science and Business Media LLC - Tập 33 - Trang 155-165 - 2010
Guang Qiu1,2,3, Ruiqian Wan4, Jingping Hu3, Mark P. Mattson4, Edward Spangler3, Shan Liu5, Suk-Yu Yau2, Tatia M. C. Lee6, Marc Gleichmann4, Donald K. Ingram7, Kwok-Fai So2, Sige Zou3
1Department of Neurology, Nanfang Hospital, Nanfang Medical University, Guangzhou, China
2Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, China
3Laboratory of Experimental Gerontology, National Institute on Aging, Baltimore, USA
4Laboratory of Neurosciences, National Institute on Aging, Baltimore, USA
5Department of Nursing, Faculty of Medicine, JiNan University, Guangzhou, China
6Laboratory of Neuropsychology and Laboratory of Cognitive Affective Neuroscience, HKU Alzheimer’s Disease Research Network, University of Hong Kong, Pokfulam, China
7Nutritional Neuroscience and Aging Laboratory, Pennington Biomedical Research Center, Baton Rouge, USA

Tóm tắt

Adiponectin exerts multiple regulatory functions in the body and in the hypothalamus primarily through activation of its two receptors, adiponectin receptor1 and adiponectin receptor 2. Recent studies have shown that adiponectin receptors are widely expressed in other areas of the brain including the hippocampus. However, the functions of adiponectin in brain regions other than the hypothalamus are not clear. Here, we report that adiponectin can protect cultured hippocampal neurons against kainic acid-induced (KA) cytotoxicity. Adiponectin reduced the level of reactive oxygen species, attenuated apoptotic cell death, and also suppressed activation of caspase-3 induced by KA. Pretreatment of hippocampal primary neurons with an AMPK inhibitor, compound C, abolished adiponectin-induced neuronal protection. The AMPK activator, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside, attenuated KA-induced caspase-3 activity. These findings suggest that the AMPK pathway is critically involved in adiponectin-induced neuroprotection and may mediate the antioxidative and anti-apoptotic properties of adiponectin.

Tài liệu tham khảo

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