Adaptive optical microscopy via virtual-imaging-assisted wavefront sensing for high-resolution tissue imaging

PhotoniX - 2022
Zhou Zhou1, Jiangfeng Huang1, Xiang Li1, Xiujuan Gao1, Zhongyun Chen1, Zhenfei Jiao1, Zhihong Zhang2, Luo Qinghong3, Ling Fu3
1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, 430074, China
2Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology, Wuhan, 430074, China
3MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, 430074, China

Tóm tắt

AbstractAdaptive optics (AO) is a powerful tool for optical microscopy to counteract the effects of optical aberrations and improve the imaging performance in biological tissues. The diversity of sample characteristics entails the use of different AO schemes to measure the underlying aberrations. Here, we present an indirect wavefront sensing method leveraging a virtual imaging scheme and a structural-similarity-based shift measurement algorithm to enable aberration measurement using intrinsic structures even with temporally varying signals. We achieved high-resolution two-photon imaging in a variety of biological samples, including fixed biological tissues and living animals, after aberration correction. We present AO-incorporated subtractive imaging to show that our method can be readily integrated with resolution enhancement techniques to obtain higher resolution in biological tissues. The robustness of our method to signal variation is demonstrated by both simulations and aberration measurement on neurons exhibiting spontaneous activity in a living larval zebrafish.

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Tài liệu tham khảo

Kubby JA. Adaptive Optics for Biological Imaging. Boca Raton: CRC Press; 2013.

Ahn C, Hwang B, Nam K, Jin H, Woo T, Park J-H. Overcoming the penetration depth limit in optical microscopy: adaptive optics and wavefront shaping. J Innovative Optical Health Sci. 2019;12(04):1930002.

Booth MJ. Adaptive optical microscopy: the ongoing quest for a perfect image. Light Sci Appl. 2014;3(4):e165.

Ji N. Adaptive optical fluorescence microscopy. Nat Methods. 2017;14(4):374–80.

Wang K, Sun W, Richie CT, Harvey BK, Betzig E, Ji N. Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue. Nat Commun. 2015;6(1):7276.

Tao X, Fernandez B, Azucena O, Fu M, Garcia D, Zuo Y, et al. Adaptive optics confocal microscopy using direct wavefront sensing. Opt Lett. 2011;36(7):1062–4.

Azucena O, Crest J, Kotadia S, Sullivan W, Tao X, Reinig M, et al. Adaptive optics wide-field microscopy using direct wavefront sensing. Opt Lett. 2011;36(6):825–7.

Débarre D, Botcherby EJ, Watanabe T, Srinivas S, Booth MJ, Wilson T. Image-based adaptive optics for two-photon microscopy. Opt Lett. 2009;34(16):2495–7.

Débarre D, Booth MJ, Wilson T. Image based adaptive optics through optimisation of low spatial frequencies. Opt Express. 2007;15(13):8176–90.

Booth MJ. Wavefront sensorless adaptive optics for large aberrations. Opt Lett. 2007;32(1):5–7.

Ji N, Milkie DE, Betzig E. Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues. Nat Methods. 2010;7(2):141–7.

Chen W, Natan RG, Yang Y, Chou S-W, Zhang Q, Isacoff EY, et al. In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution. Nat Commun. 2021;12(1):6630.

Ji N, Sato TR, Betzig E. Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex. Proc Natl Acad Sci. 2012;109(1):22.

Milkie DE, Betzig E, Ji N. Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination. Opt Lett. 2011;36(21):4206–8.

Wang C, Liu R, Milkie DE, Sun W, Tan Z, Kerlin A, et al. Multiplexed aberration measurement for deep tissue imaging in vivo. Nat Methods. 2014;11(10):1037–40.

Maurer C, Jesacher A, Bernet S, Ritsch-Marte M. What spatial light modulators can do for optical microscopy. Laser Photonics Rev. 2011;5(1):81–101.

Gould TJ, Burke D, Bewersdorf J, Booth MJ. Adaptive optics enables 3D STED microscopy in aberrating specimens. Opt Express. 2012;20(19):20998–1009.

Patton BR, Burke D, Owald D, Gould TJ, Bewersdorf J, Booth MJ. Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics. Opt Express. 2016;24(8):8862–76.

Wang P, Slipchenko MN, Mitchell J, Yang C, Potma EO, Xu X, et al. Far-field imaging of non-fluorescent species with subdiffraction resolution. Nat Photonics. 2013;7(6):449–53.

Zhao G, Rong Z, Kuang C, Zheng C, Liu X. 3D fluorescence emission difference microscopy based on spatial light modulator. J Innov Optical Health Sci. 2016;9(03):1641003.

Tian N, Fu L, Gu M. Resolution and contrast enhancement of subtractive second harmonic generation microscopy with a circularly polarized vortex beam. Sci Rep. 2015;5(1):13580.

Southwell WH. Wave-front estimation from wave-front slope measurements. J Opt Soc Am. 1980;70(8):998–1006.

Panagopoulou SI, Neal DP. Zonal matrix iterative method for wavefront reconstruction from gradient measurements. J Refract Surg. 2005;21(5):S563–S9.

Kuang C, Li S, Liu W, Hao X, Gu Z, Wang Y, et al. Breaking the diffraction barrier using fluorescence emission difference microscopy. Sci Rep. 2013;3(1):1441.

Dehez H, Piché M, De Koninck Y. Resolution and contrast enhancement in laser scanning microscopy using dark beam imaging. Opt Express. 2013;21(13):15912–25.

Rodríguez C, Chen A, Rivera JA, Mohr MA, Liang Y, Natan RG, et al. An adaptive optics module for deep tissue multiphoton imaging in vivo. Nat Methods. 2021;18(10):1259–64.

Wang C, Ji N. Pupil-segmentation-based adaptive optical correction of a high-numerical-aperture gradient refractive index lens for two-photon fluorescence endoscopy. Opt Lett. 2012;37(11):2001–3.

Leutenegger M, Rao R, Leitgeb RA, Lasser T. Fast focus field calculations. Opt Express. 2006;14(23):11277–91.

Zipfel WR, Williams RM, Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences. Nat Biotechnol. 2003;21(11):1369–77.

Zhou W, Bovik AC, Sheikh HR, Simoncelli EP. Image quality assessment: from error visibility to structural similarity. IEEE Trans Image Process. 2004;13(4):600–12.

Qi S, Li H, Lu L, Qi Z, Liu L, Chen L, et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. eLife. 2016;5:e14756.

Shen Z, Lu Z, Chhatbar PY, O'Herron P, Kara P. An artery-specific fluorescent dye for studying neurovascular coupling. Nat Methods. 2012;9(3):273–6.

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