Adaptation of a Silica Membrane-based Kit for Purification of Sequencing-ready BAC DNA

Plant Molecular Biology Reporter - Tập 25 - Trang 11-16 - 2007
C. Eduardo Vallejos1, Ney S. Sakiyama2, Valerie Jones1, Savita Shanker3
1Department of Horticultural Sciences and Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, USA
2Departamento de Fitotecnia, Universidade Federal de Viçosa, Viçosa, Brazil
3DNA Sequencing Laboratory, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, USA

Tóm tắt

We describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.

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Plant Molecular Biology Reporter

Tập 25

11-16

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