Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods
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Loens K, Ursi D, Goossens H, Ieven M (2003) Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections. J Clin Microbiol 41:4915–4923
Loens K, Beck T, Ursi D, Pattyn S, Goossens H, Ieven M (2006) Two quality control exercises involving nucleic acid amplification methods for detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae and carried out 2 years apart (in 2002 and 2004). J Clin Microbiol 44:899–908
Dowell SF, Peeling RW, Boman J, Carlone GM, Fields BS, Guarner J, Hammerschlag MR, Jackson LA, Kuo CC, Maass M, Messmer TO, Talkington DF, Tondella ML, Zaki SR (2001) Standardizing Chlamydia pneumoniae assays: recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada). Clin Infect Dis 33:492–503
Foy HM (1993) Infections caused by Mycoplasma pneumoniae and possible carrier state in different populations of patients. Clin Infect Dis 17(Suppl 1):S37–S46
Mundy LM, Auwaerter PG, Oldach D, Warner ML, Burton A, Vance E, Gaydos CA, Joseph JM, Gopalan R, Moore RD (1995) Community-acquired pneumonia: impact of immune status. Am J Respir Crit Care Med 152:1309–1315
van Kuppeveld FJ, Johansson KE, Galama JM, Kissing J, Bölske G, Hjelm E, van der Logt JT, Melchers WJ (1994) 16S rRNA based polymerase chain reaction compared with culture and serological methods for diagnosis of Mycoplasma pneumoniae infection. Eur J Clin Microbiol Infect Dis 13:401–405
Nagalingam NA, Adesiyun AA, Swanston WH, Bartholomew M (2004) Prevalence of Mycoplasma pneumoniae and Chlamydia pneumoniae in pneumonia patients in four major hospitals in Trinidad. New Microbiol 27:345–351
Oosterheert JJ, van Loon AM, Schuurman R, Hoepelman AI, Hak E, Thijsen S, Nossent G, Schneider MM, Hustinx WM, Bonten MJ (2005) Impact of rapid detection of viral and atypical bacterial pathogens by real-time polymerase chain reaction for patients with lower respiratory tract infection. Clin Infect Dis 41:1438–1444
Don M, Fasoli L, Paldanius M, Vainionpää R, Kleemola M, Räty R, Leinonen M, Korppi M, Tenore A, Canciani M (2005) Aetiology of community-acquired pneumonia: serological results of a paediatric survey. Scand J Infect Dis 37:806–812
Lehtinen P, Jartti T, Virkki R, Vuorinen T, Leinonen M, Peltola V, Ruohola A, Ruuskanen O (2006) Bacterial coinfections in children with viral wheezing. Eur J Clin Microbiol Infect Dis 25:463–469
Shenoy VD, Upadhyaya SA, Rao SP, Shobha KL (2005) Mycoplasma pneumoniae infection in children with acute respiratory infection. J Trop Pediatr 51:232–235
Gruteke P, Glas AS, Dierdorp M, Vreede WB, Pilon JW, Bruisten SM (2004) Practical implementation of a multiplex PCR for acute respiratory tract infections in children. J Clin Microbiol 42:5596–5603
Liu G, Talkington DF, Fields BS, Levine OS, Yang Y, Tondella ML (2005) Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumonia. Diagn Microbiol Infect Dis 52:7–14
Gaillat J, Flahault A, deBarbeyrac B, Orfila J, Portier H, Ducroix JP, Bébéar C, Mayaud C (2005) Community epidemiology of Chlamydia and Mycoplasma pneumoniae in LRTI in France over 29 months. Eur J Epidemiol 20:643–651
Maltezou HC, La-Scola B, Astra H, Constantopoulou I, Vlahou V, Kafetzis DA, Constantopoulos AG, Raoult D (2004) Mycoplasma pneumoniae and Legionella pneumophila in community-acquired lower respiratory tract infections among hospitalized children: diagnosis by real time PCR. Scand J Infect Dis 36:639–642
Schneeberger PM, Dorigo-Zetsma JW, van der Zee A, van Bon M, van Opstal JL (2004) Diagnosis of atypical pathogens in patients hospitalized with community-acquired respiratory infection. Scand J Infect Dis 36:269–273
Sidal M, Kilic A, Unuvar E, Oguz F, Onel M, Agacfidan A, Aydin D, Koksalan K, Beka H (2007) Frequency of Chlamydia pneumoniae and Mycoplasma pneumoniae infections in children. J Trop Pediatr 53:225–231
Strålin K, Törnqvist E, Kaltoft MS, Olcén P, Holmberg H (2006) Etiologic diagnosis of adult bacterial pneumonia by culture and PCR applied to respiratory tract samples. J Clin Microbiol 44:643–645
van de Garde EM, Endeman H, van Hemert RN, Voorn GP, Deneer VH, Leufkens HG, van den Bosch JM, Biesma DH (2008) Prior outpatient antibiotic use as predictor for microbial aetiology of community-acquired pneumonia: hospital-based study. Eur J Clin Pharmacol 64:405–410
Yamazaki T, Narita M, Sasaki N, Kenri T, Arakawa Y, Sasaki T (2006) Comparison of PCR for sputum samples obtained by induced cough and serological tests for diagnosis of Mycoplasma pneumoniae infection in children. Clin Vaccine Immunol 13:708–710
Kogan R, Martínez MA, Rubilar L, Payá E, Quevedo I, Puppo H, Girardi G, Castro-Rodriguez JA (2003) Comparative randomized trial of azithromycin versus erythromycin and amoxicillin for treatment of community-acquired pneumonia in children. Pediatr Pulmonol 35:91–98
Loens K, Beck T, Ursi D, Overdijk M, Sillekens P, Goossens H, Ieven M (2008) Evaluation of different nucleic acid amplification techniques for the detection of M. pneumoniae, C. pneumoniae and Legionella spp. in respiratory specimens from patients with community-acquired pneumonia. J Microbiol Methods 73:257–262
Miyashita N, Ouchi K, Kawasaki K, Oda K, Kawai Y, Shimizu H, Kobashi Y, Oka M (2008) Mycoplasma pneumoniae pneumonia in the elderly. Med Sci Monit 14:CR387–CR391
Ozaki T, Nishimura N, Ahn J, Watanabe N, Muto T, Saito A, Koyama N, Nakane K, Funahashi K (2007) Utility of a rapid diagnosis kit for Mycoplasma pneumoniae pneumonia in children, and the antimicrobial susceptibility of the isolates. J Infect Chemother 13:204–207
Shankar EM, Kumarasamy N, Balakrishnan P, Saravanan S, Solomon S, Vengatesan A, Murugavel KG, Rao UA (2007) Detection of pulmonary Mycoplasma pneumoniae infections in HIV-infected subjects using culture and serology. Int J Infect Dis 11:232–238
Creer DD, Dilworth JP, Gillespie SH, Johnston AR, Johnston SL, Ling C, Patel S, Sanderson G, Wallace PG, McHugh TD (2006) Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care. Thorax 61:75–79
Martínez TM, Pino PY, Salazar BT, Jover LE, Caroca CC, Espinoza NM, Avendaño CL (2005) Diagnostic utility of the polymerase chain reaction for the diagnosis of Mycoplasma pneumoniae in elderly patients with community-acquired pneumonia. Rev Chilena Infectol 22:251–256
Nilsson AC, Björkman P, Persson K (2008) Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection. BMC Microbiol 8:93
Menéndez R, Córdoba J, de La Cuadra P, Cremades MJ, López-Hontagas JL, Salavert M, Gobernado M (1999) Value of the polymerase chain reaction assay in noninvasive respiratory samples for diagnosis of community-acquired pneumonia. Am J Respir Crit Care Med 159:1868–1873
Beersma MF, Dirven K, van Dam AP, Templeton KE, Claas EC, Goossens H (2005) Evaluation of 12 commercial tests and the complement fixation test for Mycoplasma pneumoniae-specific immunoglobulin G (IgG) and IgM antibodies, with PCR used as the “gold standard”. J Clin Microbiol 43:2277–2285
Otomo S, Yamamura J, Hayashi E, Nakamura T, Kakinuma H, Nakamoto Y, Takahashi H, Karasawa T (2008) Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratory infections by real-time PCR assay and serological tests. APMIS 116:477–483
Michelow IC, Olsen K, Lozano J, Rollins NK, Duffy LB, Ziegler T, Kauppila J, Leinonen M, McCracken GH Jr (2004) Epidemiology and clinical characteristics of community-acquired pneumonia in hospitalized children. Pediatrics 113:701–707
Nir-Paz R, Michael-Gayego A, Ron M, Block C (2006) Evaluation of eight commercial tests for Mycoplasma pneumoniae antibodies in the absence of acute infection. Clin Microbiol Infect 12:685–688
Petitjean J, Vabret A, Gouarin S, Freymuth F (2002) Evaluation of four commercial immunoglobulin G (IgG)- and IgM-specific enzyme immunoassays for diagnosis of Mycoplasma pneumoniae infections. J Clin Microbiol 40:165–171
Talkington DF, Shott S, Fallon MT, Schwartz SB, Thacker WL (2004) Analysis of eight commercial enzyme immunoassay tests for detection of antibodies to Mycoplasma pneumoniae in human serum. Clin Diagn Lab Immunol 11:862–867
Gavranich JB, Chang AB (2005) Antibiotics for community acquired lower respiratory tract infections (LRTI) secondary to Mycoplasma pneumoniae in children. Cochrane Database Syst Rev (3):CD004875
Liu FC, Chen PY, Huang FL, Tsai CR, Lee CY, Lin CF (2008) Do serological tests provide adequate rapid diagnosis of Mycoplasma pneumoniae infection? Jpn J Infect Dis 61:397–399
Vikerfors T, Brodin G, Grandien M, Hirschberg L, Krook A, Pettersson CA (1988) Detection of specific IgM antibodies for the diagnosis of Mycoplasma pneumoniae infections: a clinical evaluation. Scand J Infect Dis 20:601–610
Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH (2003) Manual of clinical microbiology, 8th edn. ASM Press, Washington
Lieberman D, Lieberman D, Ben-Yaakov M, Shmarkov O, Gelfer Y, Varshavsky R, Ohana B, Lazarovich Z, Boldur I (2002) Serological evidence of Mycoplasma pneumoniae infection in acute exacerbation of COPD. Diagn Microbiol Infect Dis 44:1–6
Lieberman D, Lieberman D, Korsonsky I, Ben-Yaakov M, Lazarovich Z, Friedman MG, Dvoskin B, Leinonen M, Ohana B, Boldur I (2002) A comparative study of the etiology of adult upper and lower respiratory tract infections in the community. Diagn Microbiol Infect Dis 42:21–28
Watkins-Riedel T, Stanek G, Daxboeck F (2001) Comparison of SeroMP IgA with four other commercial assays for serodiagnosis of Mycoplasma pneumoniae pneumonia. Diagn Microbiol Infect Dis 40:21–25
Narita M (2005) Evaluation of ELISA kits for detection of Mycoplasma pneumoniae-specific IgG, IgA, IgM antibodies on the diagnosis of Mycoplasma pneumoniae infection in children. J Jap Assoc Infect Dis 79:457–463
Csángó PA, Pedersen JE, Hess RD (2004) Comparison of four Mycoplasma pneumoniae IgM-, IgG- and IgA-specific enzyme immunoassays in blood donors and patients. Clin Microbiol Infect 10:1094–1098
Dorigo-Zetsma JW, Zaat SA, Wertheim-van Dillen PM, Spanjaard L, Rijntjes J, van Waveren G, Jensen JS, Angulo AF, Dankert J (1999) Comparison of PCR, culture, and serological tests for diagnosis of Mycoplasma pneumoniae respiratory tract infection in children. J Clin Microbiol 37:14–17
Waris ME, Toikka P, Saarinen T, Nikkari S, Meurman O, Vainionpää R, Mertsola J, Ruuskanen O (1998) Diagnosis of Mycoplasma pneumoniae pneumonia in children. J Clin Microbiol 36:3155–3159
Morton HE, Smith PF, Leberman PR (1951) Investigation of the cultivation of pleuropneumonia-like organisms from man. Am J Syph Gonorrhea Vener Dis 35:361–369
Loens K, Van Heirstraeten L, Malhotra-Kumar S, Goossens H, Ieven M (2009) Optimal sampling sites and methods for detection of pathogens possibly causing community-acquired lower respiratory tract infections. J Clin Microbiol 47:21–31
Winchell JM, Thurman KA, Mitchell SL, Thacker WL, Fields BS (2008) Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation. J Clin Microbiol 46:3116–3118
Dumke R, Jacobs E (2009) Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae. J Clin Microbiol 47:441–444
Ursi D, Ieven M, Noordhoek GT, Ritzler M, Zandleven H, Altwegg M (2003) An interlaboratory comparison for the detection of Mycoplasma pneumoniae in respiratory samples by the polymerase chain reaction. J Microbiol Methods 53:289–294
Loens K, MacKay WG, Scott C, Goossens H, Wallace P, Ieven M (2010) A multicenter pilot external quality assessment programme to assess the quality of molecular detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae. J Microbiol Methods (in press)
Lin B, Blaney KM, Malanoski AP, Ligler AG, Schnur JM, Metzgar D, Russell KL, Stenger DA (2007) Using a resequencing microarray as a multiple respiratory pathogen detection assay. J Clin Microbiol 45:443–452
Khanna M, Fan J, Pehler-Harrington K, Waters C, Douglass P, Stallock J, Kehl S, Henrickson KJ (2005) The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart. J Clin Microbiol 43:565–571
Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannès M, Pozzetto B, Grattard F (2005) Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR. J Clin Microbiol 43:3247–3254
Benson R, Tondella ML, Bhatnagar J, Carvalho Mda G, Sampson JS, Talkington DF, Whitney AM, Mothershed E, McGee L, Carlone G, McClee V, Guarner J, Zaki S, Dejsiri S, Cronin K, Han J, Fields BS (2008) Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens. J Clin Microbiol 46:2074–2077
Störmer M, Vollmer T, Henrich B, Kleesiek K, Dreier J (2009) Broad-range real-time PCR assay for the rapid identification of cell-line contaminants and clinically important mollicute species. Int J Med Microbiol 299:291–300
Wang H, Kong F, Jelfs P, James G, Gilbert GL (2004) Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization. Appl Environ Microbiol 70:1483–1486
Loens K, Bergs K, Ursi D, Goossens H, Ieven M (2007) Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens. J Clin Microbiol 45:421–425
Loens K, Ursi D, Goossens H, Ieven M (2008) Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs. J Microbiol Methods 72:217–219
Okazaki N, Narita M, Yamada S, Izumikawa K, Umetsu M, Kenri T, Sasaki Y, Arakawa Y, Sasaki T (2001) Characteristics of macrolide-resistant Mycoplasma pneumoniae strains isolated from patients and induced with erythromycin in vitro. Microbiol Immunol 45:617–620
Matsuoka M, Narita M, Okazaki N, Ohya H, Yamazaki T, Ouchi K, Suzuki I, Andoh T, Kenri T, Sasaki Y, Horino A, Shintani M, Arakawa Y, Sasaki T (2004) Characterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan. Antimicrob Agents Chemother 48:4624–4630
Morozumi M, Hasegawa K, Kobayashi R, Inoue N, Iwata S, Kuroki H, Kawamura N, Nakayama E, Tajima T, Shimizu K, Ubukata K (2005) Emergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation. Antimicrob Agents Chemother 49:2302–2306
Morozumi M, Iwata S, Hasegawa K, Chiba N, Takayanagi R, Matsubara K, Nakayama E, Sunakawa K, Ubukata K (2008) Increased macrolide resistance of Mycoplasma pneumoniae in pediatric patients with community-acquired pneumonia. Antimicrob Agents Chemother 52:348–350
Suzuki S, Yamazaki T, Narita M, Okazaki N, Suzuki I, Andoh T, Matsuoka M, Kenri T, Arakawa Y, Sasaki T (2006) Clinical evaluation of macrolide-resistant Mycoplasma pneumoniae. Antimicrob Agents Chemother 50:709–712
Pereyre S, Charron A, Renaudin H, Bébéar C, Bébéar CM (2007) First report of macrolide-resistant strains and description of a novel nucleotide sequence variation in the P1 adhesin gene in Mycoplasma pneumoniae clinical strains isolated in France over 12 years. J Clin Microbiol 45:3534–3539
Wolff BJ, Thacker WL, Schwartz SB, Winchell JM (2008) Detection of macrolide resistance in Mycoplasma pneumoniae by real-time PCR and high-resolution melt analysis. Antimicrob Agents Chemother 52:3542–3549
Morozumi M, Hasegawa K, Chiba N, Iwata S, Kawamura N, Kuroki H, Tajima T, Ubukata K (2004) Application of PCR for Mycoplasma pneumoniae detection in children with community-acquired pneumonia. J Infect Chemother 10:274–279
Templeton KE, Scheltinga SA, Graffelman AW, Van Schie JM, Crielaard JW, Sillekens P, van den Broek PJ, Goossens H, Beersma MF, Claas EC (2003) Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae. J Clin Microbiol 41:4366–4371
Ieven M, Ursi D, Van Bever H, Quint W, Niesters HG, Goossens H (1996) Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of M. pneumoniae in acute respiratory tract infections in pediatric patients. J Infect Dis 173:1445–1452
Loens K, Ieven M, Ursi D, Beck T, Overdijk M, Sillekens P, Goossens H (2003) Detection of Mycoplasma pneumoniae by real-time nucleic acid sequence-based amplification. J Clin Microbiol 41:4448–4450
Roth SB, Jalava J, Ruuskanen O, Ruohola A, Nikkari S (2004) Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections. J Clin Microbiol 42:4268–4274
Raggam RB, Leitner E, Berg J, Mühlbauer G, Marth E, Kessler HH (2005) Single-run, parallel detection of DNA from three pneumonia-producing bacteria by real-time polymerase chain reaction. J Mol Diagn 7:133–138
Saito R, Misawa Y, Moriya K, Koike K, Ubukata K, Okamura N (2005) Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae. J Med Microbiol 54:1037–1041
Ursi D, Dirven K, Loens K, Ieven M, Goossens H (2003) Detection of Mycoplasma pneumoniae in respiratory samples by real-time PCR using an inhibition control. J Microbiol Methods 55:149–153
Morozumi M, Nakayama E, Iwata S, Aoki Y, Hasegawa K, Kobayashi R, Chiba N, Tajima T, Ubukata K (2006) Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol 44:1440–1446
Pitcher D, Chalker VJ, Sheppard C, George RC, Harrison TG (2006) Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control. J Med Microbiol 55:149–155
Di Marco E, Cangemi G, Filippetti M, Melioli G, Biassoni R (2007) Development and clinical validation of a real-time PCR using a uni-molecular Scorpion-based probe for the detection of Mycoplasma pneumoniae in clinical isolates. New Microbiol 30:415–421
Kong F, Gordon S, Gilbert GL (2000) Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens. J Clin Microbiol 38:4256–4259
Sharma S, Brousseau R, Kasatiya S (1998) Detection and confirmation of Mycoplasma pneumoniae in urogenital specimens by PCR. J Clin Microbiol 36:277–280
Dumke R, Schurwanz N, Lenz M, Schuppler M, Lück C, Jacobs E (2007) Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach. J Clin Microbiol 45:2726–2730
Miyashita N, Saito A, Kohno S, Yamaguchi K, Watanabe A, Oda H, Kazuyama Y, Matsushima T (2004) Multiplex PCR for the simultaneous detection of Chlamydia pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila in community-acquired pneumonia. Respir Med 98:542–550
Briese T, Palacios G, Kokoris M, Jabado O, Liu Z, Renwick N, Kapoor V, Casas I, Pozo F, Limberger R, Perez-Brena P, Ju J, Lipkin WI (2005) Diagnostic system for rapid and sensitive differential detection of pathogens. Emerg Infect Dis 11:310–313
de Barbeyrac B, Bernet-Poggi C, Fébrer F, Renaudin H, Dupon M, Bébéar C (1993) Detection of Mycoplasma pneumoniae and Mycoplasma genitalium in clinical samples by polymerase chain reaction. Clin Infect Dis 17(Suppl 1):S83–S89
McDonough EA, Barrozo CP, Russell KL, Metzgar D (2005) A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens. Mol Cell Probes 19:314–322
Strålin K, Bäckman A, Holmberg H, Fredlund H, Olcén P (2005) Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samples. APMIS 113:99–111
Geertsen R, Kaeppeli F, Sterk-Kuzmanovic N, Andrasevic S, Anic-Milic T, Dobec M (2007) A multiplex PCR assay for the detection of respiratory bacteriae in nasopharyngeal smears from children with acute respiratory disease. Scand J Infect Dis 39:769–774
Lam WY, Yeung AC, Tang JW, Ip M, Chan EW, Hui M, Chan PK (2007) Rapid multiplex nested PCR for detection of respiratory viruses. J Clin Microbiol 45:3631–3640
Lodes MJ, Suciu D, Wilmoth JL, Ross M, Munro S, Dix K, Bernards K, Stöver AG, Quintana M, Iihoshi N, Lyon WJ, Danley DL, McShea A (2007) Identification of upper respiratory tract pathogens using electrochemical detection on an oligonucleotide microarray. PLoS ONE 2:e924
Gullsby K, Storm M, Bondeson K (2008) Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR. J Clin Microbiol 46:727–731
van Kuppeveld FJ, van der Logt JT, Angulo AF, van Zoest MJ, Quint WG, Niesters HG, Galama JM, Melchers WJ (1992) Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 58:2606–2615
Loens K, Beck T, Ursi D, Overdijk M, Sillekens P, Goossens H, Ieven M (2008) Development of real-time multiplex nucleic acid sequence-based amplification for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. in respiratory specimens. J Clin Microbiol 46:185–191
Wang Y, Kong F, Yang Y, Gilbert GL (2008) A multiplex PCR-based reverse line blot hybridization (mPCR/RLB) assay for detection of bacterial respiratory pathogens in children with pneumonia. Pediatr Pulmonol 43:150–159
Kumar S, Wang L, Fan J, Kraft A, Bose ME, Tiwari S, Van Dyke M, Haigis R, Luo T, Ghosh M, Tang H, Haghnia M, Mather EL, Weisburg WG, Henrickson KJ (2008) Detection of 11 common viral and bacterial pathogens causing community-acquired pneumonia or sepsis in asymptomatic patients by using a multiplex reverse transcription-PCR assay with manual (enzyme hybridization) or automated (electronic microarray) detection. J Clin Microbiol 46:3063–3072