Acute administration of levetiracetam in tonic pain model modulates gene expression of 5HT1A and 5HT7 receptors in the thalamus of rats (Rattus norvergicus)

Springer Science and Business Media LLC - Tập 47 - Trang 3389-3396 - 2020
José Luis Cortes-Altamirano1,2, Samuel Reyes-Long1,3, Herlinda Bonilla-Jaime4, Denise Clavijo-Cornejo5, Javier Vargas1, Cindy Bandala1,3, Alfonso Alfaro-Rodriguez1,6
1División de Neurociencias, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra” (INR) Secretaría de Salud (SSA), Mexico City, México
2Departamento de Quiropráctica, Universidad Estatal del Valle de Ecatepec (UNEVE), Mexico City, México
3Escuela Superior de Medicina, Instituto Politécnico Nacional (IPN), Mexico City, México
4Departamento de Biología de La Reproducción, Universidad Autónoma Metropolitana (UAM) Campus Iztapalapa, Mexico City, México
5División de Enfermedades Musculoesqueléticas Y Reumáticas, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra” (INR) Secretaría de Salud (SSA), Mexico City, México
6Neuropharmacology, Departament of Neurosciences, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra Ibarra”, Mexico City, México

Tóm tắt

The nociceptive effect of Levetiracetam (LEV) on the expression of 5-HT1A and 5-HT7 receptors found in the thalamus was evaluated. Thirty-six male rats (Wistar) were randomized into six groups: in the Control group without treatment; LEV50 group LEV was administered in a single dose of 50 mg/kg i.g.; in the LEV300 group LEV dose of 300 mg/kg i.g.; in the FORMALIN group the formalin test was performed; in the LEV50/FORMALIN group LEV dose of 50 mg/kg i.g and the formalin test was performed; in the LEV300/FORMALIN group LEV dose of 300 mg/kg i.g and the formalin test was performed, subsequently the thalamus was dissected in all groups. In the formalin tests LEV exhibited an antinociceptive effect in the LEV300/FORMALIN group (p < 0.05) and a pronociceptive effect in the LEV50/FORMALIN group (p < 0.001). The results obtained by Real-time PCR confirmed the expression of the 5-HT1A and 5-HT7 receptors in the thalamus, 5-HT1A receptors increased significantly in the FORMALIN group and the LEV300/FORMALIN group (p < 0.05). 5-HT7 receptors are only over expressed at a dose of 300 mg/Kg of LEV with formalin (p < 0.05). This suggests that LEV modulates the sensation of pain by controlling the expression of 5-HT1A and 5-HT7 in a tonic pain model, and that changes in the expression of 5-HT1A and 5-HT7 receptors are associated with the sensation of pain, furthermore its possibility to be used in clinical treatments for pain.

Tài liệu tham khảo

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