Activity‐dependent regulation of the sumoylation machinery in rat hippocampal neurons

Biology of the Cell - Tập 105 Số 1 - Trang 30-45 - 2013
Céline Loriol1, Anouar Khayachi1, Gwénola Poupon1, Carole Gwizdek1, Stéphane Martin1
1Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR‐7275, University of Nice Sophia‐Antipolis, Valbonne 06560, France

Tóm tắt

AbstractBackground informationSumoylation is a key post‐translational modification by which the Small Ubiquitin‐like MOdifier (SUMO) polypeptide is covalently attached to specific lysine residues of substrate proteins through a specific enzymatic pathway. Although sumoylation participates in the regulation of nuclear homeostasis, the sumoylation machinery is also expressed outside of the nucleus where little is still known regarding its non‐nuclear functions, particularly in the Central Nervous System (CNS). We recently reported that the sumoylation process is developmentally regulated in the rat CNS.ResultsHere, we demonstrate that there is an activity‐dependent redistribution of endogenous sumoylation enzymes in hippocampal neurons. By performing biochemical and immunocytochemical experiments on primary cultures of rat hippocampal neurons, we show that sumoylation and desumoylation enzymes are differentially redistributed in and out of synapses upon neuronal stimulation. This enzymatic redistribution in response to a neuronal depolarisation results in the transient decrease of sumoylated protein substrates at synapses.ConclusionsTaken together, our data identify an activity‐dependent regulation of the sumoylation machinery in neurons that directly impacts on synaptic sumoylation levels. This process may provide a mechanism for neurons to adapt their physiological responses to changes occurring during neuronal activation.

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Biology of the Cell

Tập 105 Số 1

30-45

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