Activation of Transcription Factors and IL-8 Expression in Neutrophils Stimulated with Lipopolysaccharide from Porphyromonas gingivalis
Tóm tắt
DNA binding activity of NF-κB and AP-1 were examined in neutrophils stimulated with LPS purified from P. gingivalis, a major pathogenic bacteria of periodontitis lesion. Porphyromonas gingivalis LPS enhanced the activity reaching a peak at a concentration of 500 ng/ml in the absence of serum. The NF-κB activation stimulated with 10 ng/ml of P. gingivalis LPS was suppressed approximately 44% by treatment of neutrophils with anti-CD14 antibody under the presence of serum. Increase in the steady-state IL-8 mRNA level was concomitantly observed by stimulation of neutrophils with 500 ng/ml of P. gingivalis LPS under the absence of serum. These results indicate that P. gingivalis LPS activates NF-κB and AP-1 in both serum-dependent and -independent manners, followed by increased IL-8 transcription in neutrophils, and suggested a role for P. gingivalis LPS in IL-8 synthesis by neutrophils in inflamed gingiva and GCF.
Tài liệu tham khảo
Dubravec, D. B., D. R. Spriggs, J. A. Mannick, and M. L. Rodrick. 1990. Circulating human peripheral blood granulocytes synthesize and secrete tumor necrosis factor α. Proc. Natl. Acad. Sci. U.S.A. 87:6758–6761.
Strieter, R. M., K. Kasahara, R. M. Allen, T. J. Standiford, M. W. Rolfe, F. S. Becker, S. W. Chensue, and S. L. Kunkel. 1992. Cytokine-induced neutrophil-derived interleukin-8. Am. J. Pathol. 141:397–407.
Cicco, N. A., A. Lindemann, J. Content, P. Vandenbussche, M. LÜbbert, J. Gauss, R. Mertelsmann, and F. Herrmann. 1990. Inducible production of interleukin-6 by human polymorphonuclear neutrophils: Role of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-alpha. Blood 75:2049–2052.
Lloyd, A. R., and J. J. Oppenheim. 1992. Poly's lament: the neglected role of the polymorphonuclear neutrophil in the afferent limb of the immune response. Immunol. Today 13:169–172.
Liu, J. H., and J. Y. Djeu. 1995. Role of cytokines in neutrophil functions. In Human Cytokines: Their Role in Disease and Therapy. (B. B. Aggarwal and R. K. Puri, eds.), Blackwell Science, Cambridge. 71–86.
Sugita, N., T. Suzuki, H. Yoshie, N. Yoshida, M. Adachi, and K. Hara. 1993. Differential expression of CR3, FeeRII and Fc γ RIII on polymorphonuclear leukocytes in gingival crevicular fluid. J. Periodont. Res. 28:363–372.
Aida, Y., T. Kukita, H. Takada, K. Maeda, and M. J. Pabst. 1995. Lipopolysaccharides from periodontal pathogens prime neutrophils for enhanced respiratory burst: differential effect of a synthetic lipid a precursor IVA (LA-14-PP). J. Periodont. Res. 30:116–123.
Wilson, M. 1993. Biological activities of lipopolysaccharide and endotoxin. In Biology of the species Porphyromonas gingivalis (H. N. Shah, D. Mayrand and R. J. Genco, eds.), CRC Press, Boca Raton, Florida 171–197.
Shapira, L., S. Takashiba, C. Champagne, S. Amar and T. E. Van Dyke. 1994. Involvement of protein kinase C and protein tyrosine kinase in lipopolysaccharide-induced TNF-α and IL-1β production by human monocytes. J. Immunol. 153:1818–1824.
Koga, T., T. Nishihara, T. Fujiwara, T. Nisizawa, N. Okahashi, T. Noguchi, and S. Hamada. 1985. Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli. Infect. Immun. 47:638–647.
McDonald, P. P., A. Bald and M. A. Cassatella. 1997. Activation of the NF-κB pathway by inflammatory stimuli in human neutrophils. Blood 89:3421–3433.
Mukaida, N., S. Okamoto, Y. Ishikawa, and K. Matsushima. 1994. Molecular mechanism of interleukin-8 gene expression. J. Leukoc. Biol. 56:554–558.
Tamura, H., S. Tanaka, T. Obayashi, M. Yoshida, and T. Kawai. 1992. A new sensitive microplate assay of plasma endotoxin. J. Clin. Lab. Anal. 6:232–238.
English, D., and B. R. Andersen. 1974. Single-step separation of red blood cells, granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque. J. Immunol. Methods 5:249–252.
Meda, L., S. Gasperini, M. Ceska, and M. A. Cassatella. 1994. Modulation of proinflammatory cytokine release from human polymorphonuclear leukocytes by gamma interferon. Cell. Immunol. 157:448–461.
Shapira, L., S. Takashiba, S. Amar and T. E. Van Dyke. 1994. Porphyromonas gingivalis lipopolysaccharide stimulation of human monocytes: dependence on serum and CD14 receptor. Oral Microbiol. Immunol. 9:112–117.
Osborn, L., S. Kunkel, and G. J. Nabel. 1989. Tumor necrosis factor α and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor κB. Proc. Natl. Acad. Sci. U.S.A. 84:2336–2340.
Thieblemont, N., N. Haeffner-Cavaillon, A. Haeffner, B. Cholley, L. Weiss, and M. D. Kazatchkine. 1995. Triggering of complement receptors CR1 (CD35) and CR3 (CD11b/CD18) induces nuclear translocation of NF-κB (p50/p65) in human monocytes and enhances viral replication of HIV-infected monocytic cells. J. Immunol. 155:4861–4867.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Anal. Biochem. 72:248–254.
Adcock, I. M., S. J. Lane, C. R. Brown, M. J. Peters, T. H. Lee, and P. J. Barnes. 1995. Differences in binding of glucocorticoid receptor to DNA in steroid-resistant asthma. J. Immunol. 154:3500–3505.
Nomura, T., T. Takahashi and K. Hara. 1993. Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue. J. Periodont. Res. 28:354–362.
Sugita, N., Y. Matsuki, H. Yoshie, and K. Hara. 1997. Characterization of complement receptor type 1 and type 3 mRNA expression on polymorphonuclear leucocytes (neutrophils) in gingival crevicular fluid from periodontitis-affected patients. Arch. Oral Biol. 42:113–120.
Chelly, J., J. C. Kaplan, P. Maire, S. Gautron, and A. Kahn. 1988. Transcription of the dytrophin gene in human muscle and non-muscle tissues. Nature 333:858–860.
Tokoro, Y., T. Yamamoto, and K. Hara. 1996. IL-1β mRNA as the predominant inflammatory cytokine transcript: correlation with inflammatory cell infiltration into human gingiva. J. Oral Pathol. Med. 25:225–231.
Ishikawa, Y., N. Mukaida, K. Kuno, N. Rice, S. Okamoto, and K. Matsushima. 1995. Establishment of lipopolysaccharide-dependent nuclear factor κB activation in a cell-free system. J. Biol. Chem. 270:4158–4164.
Ding, A., S. Hwang, H. M. Lander, and Q. W. Xie. 1995. Macrophages derived from C3H/HeJ (Lpsd) mice respond to bacterial lipopolysaccharide by activating NF-κB. J. Leukoc. Biol. 57:174–179.
Matsusaka, T., K. Fujikawa, Y. Nishio, N. Mukaida, K. Matsushima, T. Kishimoto, and S. Akira. 1993. Transcription factors NF-IL6 and NF-κB synergistically activate transcription of the inflammatory cytokines, interleukin 6 and interleukin 8. Proc. Natl. Acad. Sci. U.S.A. 90:10193–10197.
Baeuerle, P. A., and T. Henkel. 1994. Function and activation of NF-κB in the immune system. Annu. Rev. Immunol. 12:141–179.
Fujishima, S., A. R. Hoffman, T. Vu, K. J. Kim, H. Zheng, D. Daniel, Y. Kim, E. F. Wallace, J. W. Larrick, and T. A. Raffin. 1993. Regulation of neutrophil interleukin 8 gene expression and protein secretion by LPS, TNF-α, and IL-1β. J. Cell. Physiol. 154:478–485.
Wright, S. D., R. A. Ramos, P. S. Tobias, R. J. Ulevitch, and J. C. Mathison. 1990. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249:1431–1433.
Schumann, R. R., S. R. Leong, G. W. Flaggs, P. W. Gray, S. D. Wright, J. C. Mathison, P. S. Tobias, and R. J. Ulevitch. 1990. Structure and function of lipopolysaccharide binding protein. Science 249:1429–1431.
Simmons, D. L., S. Tan, D. G. Tenen, A. Nicholson-Weller and B. Seed. 1989. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood 73:284–289.
Haziot, A., B. Z. Tsuberi, and S. M. Goyert. 1993. Neutrophil CD14: Biochemical properties and role in the secretion of tumor necrosis factor-α in response to lipopolysaccharide. J. Immunol. 150:5556–5565.
Mukaida, N., Y. Ishikawa, N. Ikeda, N. Fujioka, S. Watanabe, K. Kuno, and K. Matsushima. 1996. Novel insight into molecular mechanism of endotoxin shock: biochemical analysis of LPS receptor signaling in a cell-free system targeting NF-κB and regulation of cytokine production/action through β2 integrin in vivo. J. Leukoc. Biol. 59:145–151.
Shapira, L., C. Champagne, B. Gordon, S. Amar, and T. E. Van Dyke. 1995. Lipopolysaccharide priming of superoxide release by human neutrophils: Role of membrane CD14 and serum LPS binding protein. Inflammation 19:289–295.
Hailman, E., T. Vasselon, M. Kelley, L. A. Busse, M. C. T. Hu, H. S. Lichenstein, P. A. Detmers, and S. D. Wright. 1996. Stimulation of macrophages and neutrophils by complexes of lipopolysaccharide and soluble CD14. J. Immunol. 156:4384–4390.
Wright, S. D., S. M. Levin, M. T. C. Jong, Z. Chad, and L. G. Kabbash. 1989. CR3 (CD11b/CD18) expresses one binding site for Arg-Gly-Asp-containing peptides and a second site for bacterial lipopolysaccharide. J. Exp. Med. 169:175–183.
Nair, B. C., W. R. Mayberry, R. Dziak, P. B. Chen, M. J. Levine, and E. Hausmann. 1983. Biological effects of a purified lipopolysaccharide from Bacteroides gingivalis. J. Periodont. Res. 18:40–49.
Harada, A., N. Sekido, T. Akahoshi, T. Wada, N. Mukaida, and K. Matsushima. 1994. Essential involvement of interleukin-8 (IL-8) in acute inflammation. J. Leukoc. Biol. 56:559–564.
Beaulieu, A. D., and S. R. McColl. 1994. Differential expression of two major cytokines produced by neutrophils, interleukin-8 and the interleukin-1 receptor antagonist, in nuetrophils isolated from the synovial fluid and peripheral blood of patients with rheumatoid arthritis. Arthritis Rheum. 57:855–859.
Tonetti, M. S., M. A. Imboden, L. Gerber, N. P. Lang, J. Laissue, and C. Mueller. 1994. Localized expression of mRNA for phagocyte-specific chemotactic cytokines in human periodontal infections. Infect. Immun. 62:4005–4014.
Furie, M. B., and G. J. Randolph. 1995. Chemokines and tissue injury. Am. J. Pathol. 146:1287–1301.
Gura, T. 1996. Chemokines take center stage in inflammatory ills. Science 272:954–956.