Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Lipoprotein tỷ trọng thấp được acetyl hóa được nội bào hóa qua các vị trí phủ bởi đại thực bào khoang bụng chuột
Tóm tắt
Phân bố bề mặt của các thụ thể scavenger đối với lipoprotein tỷ trọng thấp acetyl hóa (acetyl-LDL) và hành vi nội bào của chúng đã được nghiên cứu bằng phương pháp miễn dịch peroxidase trực tiếp, sử dụng các hợp chất monomer của peroxidase củ cải đường với kháng thể Fab’ được tạo ra chống lại LDL. Các thụ thể được chứng minh là phân bố rải rác trên màng bề mặt của các đại thực bào khoang bụng nuôi cấy, với sự phân bố ưu tiên ở các vùng hố phủ. Khi nhiệt độ thay đổi từ 4°C lên 37°C, acetyl-LDL liên kết với màng bề mặt nhanh chóng biến mất, nhưng có thể phát hiện trong các túi bọc phủ hoặc lysosome. Sự ủ tiếp theo trong sự hiện diện của acetyl-LDL cho thấy các vacuole lipid không có màng bao bọc trong bào tương, biến đổi các đại thực bào thành các tế bào bọt điển hình. Những dữ liệu này gợi ý rằng việc gắn acetyl-LDL vào các thụ thể của nó kích thích sự tập hợp các thụ thể vào các vùng hố phủ, nơi mà acetyl-LDL được nội bào hóa bởi các túi bọc phủ để bị phân hủy trong lysosome và dẫn đến sự tích lũy cholesterol este nội bào.
Từ khóa
#acetyl-LDL #thụ thể scavenger #đại thực bào #nội bào hóa #hố phủ #lysosome #cholesterol esteTài liệu tham khảo
Assman A, Frederickson DS (1983) Acid lipase defficiency: Wolman’s desease & cholesteryl ester storage disease. In: Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS (eds) The metabolic basis of inheritated disease. McGraw-Hill, New York, pp 803–819
Brandy RO (1983) Sphingomyelin lipidoses: Niemann-Pick Disease. In: Stanbury JB, Wyngaarden JB, Fredrickson DS, Goldstein JL, Brown MS (eds) The metabolic basis of inheritated disease. McGraw-Hill, New York, pp 831–841
Brown MS, Goldstein JL (1983) Lipoprotein metabolism in the macrophage: Implication for cholesterol deposition in atherosclerosis. Annu Rev Biochem 52:223–261
Brown MS, Goldstein JL, Krieger M, Ho YK, Anderson RGW (1979) Reversible accumulation of cholesterol esters in macrophages incubated with acetylated lipoproteins. J Cell Biol 82:597–613
Chao Y-S, Jones AL, Hradek GT, Windier EE, Havel RJ (1981) Autoradiographic localization of the sites of uptake, cellular transport, and catabolism of low density lipoproteins in the liver of normal and estrogen-treated rats. Proc Natl Acad Sci (USA) 78:597–601
Fogelman AM, Shechter I, Seager J, Hokom M, Child JS, Edwards PA (1980) Malondialdehyde alteration of low density lipoprotein leads to cholesterol ester accumulation in human monocyte-macrophages. Proc Natl Acad Sci (USA) 77:2214–2218
Goldstein JL, Brown MS (1977) The low-density lipoprotein pathway and its relation to atherosclerosis. Annu Rev Biochem 46:897–930
Goldstein JL, Ho YK, Basu SK, Brown MS (1979a) Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc Natl Acad Sci (USA) 76:333–337
Goldstein JL, Anderson RGW, Brown MS (1979 b) Coated pits, coated vesicles, and receptormediated endocytosis. Nature 279:679–685
Gomori G (1950) An improved histochemical technique for acid phosphatase. Stain Tech 25:81–85
Henrickson T, Mahoney EM, Steinberg D (1981) Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells: Recognition by receptors for acetylated low density lipoprotein. Proc Natl Acad Sci (USA) 78:6499–6503
Horiuchi S, Takata K, Maeda H, Morino Y (1985 a) Scavenger function of sinusoidal liver cells: Acetylated low-density lipoprotein is endocytosed via a route distinct from formaldehyde-treated serum albumin. J Biol Chem 260:53–56
Horiuchi S, Kamimoto Y, Morino Y (1985 b) Hepatic clearance of rat liver aspartate aminotransferase isozymes: Evidence for endocytic uptake via different binding sites on sinusoidal liver cells. Hepatology 5:376–382
Hornick CA, Hamilton RL, Spaziani E, Enders GH, Havel RJ (1985) Isolation and characterization of multivesicular bodies from rat hepatocytes: An organelle distinct from secretary vesicles of the golgi apparatus. J Cell Biol 100:1558–1569
Imagawa M, Yoshitake S, Hamaguchi Y, Ishikawa E, Niitsu Y, Urushizawa I, Kanazawa R, Tachibana S, Nakazawa N, Ogawa H (1982) Characteristics and evaluation of antibodyhorseradish peroxidase conjugates prepared by using a maleimide compound, glutaraldehyde, and a periodate. J Appl Biochem 4:41–57
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Mahley RW, Innerarity TL, Weisgraber KH, Oh SY (1979) Altered metabolism (in vivo andin vitro) of plasma lipoprotein after selective chemical modification of lysine residues of the apoproteins. J Clin Invest 64:743–750
Miller R, Bialer MG, Rogers JF, Jonsson HT Jr, Allen RV, Hennigar GR (1982) Wolman’s disease. Report of a case, with multiple studies. Arch Pathol Lab Med 106:415
Paavola LG, Strauss JF, Boyd CO, Nestler JE (1985) Uptake of gold- and [3H]cholesterol linoleate-labeled human low density lipoprotein by cultured rat granulosa cells: Cellular mechanisms involved in lipoprotein metabolism and their importance to steroidgenesis. J Cell Biol 100:1235–1247
Robenek H, Schmitz G, Assmann G (1984) Topography and dynamics of receptors for acetylated and malondialdehyde-modified low-density lipoprotein in the plasma membrane of mouse peritoneal macrophages as visualized by colloidal gold in conjugation with surface replicas. J Histochem Cytochem 32:1017–1027
Schaffner T, Tayler K, Bartucci E, Fischer-Dzoga K, Beeson J, Glagov S, Wissler R (1980) Arterial foam cells with distinctive immunomorphological and histochemical features of macrophages. Am J Pathol 100:57–80
Seno S, Ono T, Tsuji T (1983) Macromolecular charge and cellular surface charge in adhesion, ingestion, and blood vessel leakage. Ann NY Acad Sci 416:410–425
Shechter I, Fogelmann AM, Haberland ME, Seager I, Hokom M, Edwards PA (1981) The metabolism of native and malondialdehyde-altered low density lipoprotein by human monocyte-macrophages. J Lipid Res 22:63–71
Shio H, Farquhar MG, de Duve C (1974) Lysosomes of the arterial wall. IV. Cytochemical localization of acid phosphatase and catalase in smooth muscle cells and foam cells from rabbit atheromatous aorta. Am J Pathol 76:1–16
Silverstein SC, Steinmann RM, Cohn ZA (1977) Endocytosis. Annu Rev Biochem 46:669–722
Takahashi K, Naito M (1983) Lipid storage disease: Part I. Ultrastructure of xanthoma cells in various xanthomatous diseases. Acta Pathol Jpn 33:959–977
Takahashi K, Naito M (1985) Lipid storage disease: Part II. Ultrastructural pathology of lipid storage cells in sphingolipidoses. Acta Pathol Jpn 35:385–408
Traber MG, Kallman B, Kayden HJ (1983) Localization of the binding site of native and acetylated low-density lipoprotein (LDL) in human monocyte-derived macrophages. Exp Cell Res 148:281–292
Tsuruta J, Yamamoto T, Kozono K, Kambara T (1985) Application of a new method of antibody-enzyme conjugation with maleimide derivative for immunohistochemistry: Hepatocellular production, interstitial tissue distribution, and renal cell reabsorption of plasma albumin in guinea pig. J Histochem Cytochem 33:767–777
Unkeless JC, Fleit H, Mellman IS (1981) Structural aspects and heterogeneity of immunoglobulin Fc receptors. Adv Immunol 31:247–270
van Der Schroeff JG, Havekes L, Emeis JJ, Wijsman M, van Der Meer H, Vermer BJ (1983) Morphological studies on the binding site of low-density lipoproteins and acetylated lowdensity lipoproteins to the plasma membrane of cultured monocytes. Exp Cell Res 145:95–103
Via DP, Plant AL, Craig IF, Gotto AM, Jr (1985 a) Metabolism of normal and modified low-density lipoprotein by macrophage cell lines of murine and human origin. Biochim Biophys Acta 833:417–428
Via DP, Dresel HA, Cheng S-L, Gotto AM, Jr (1985b) Murine macrophage tumors are a source of a 260,000-dalton acetyl-low density lipoprotein receptor. J Biol Chem 260:7379–7386
Willingham MC (1983) An alternative fixation-processing method for preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: The GBS (glutaraldehyde-borohydride-saponin) procedure. J Histochem Cytochem 31:791–798
Willingham MC, Pastan IH (1982) Transit of epidermal growth factor through coated pit of the golgi system. J Cell Biol 94:207–212
Willingham MC, Maxfield FR, Pastan IH (1979) α2Macroglobulin binding to the plasma membrane of cultured fibroblasts: Diffuse binding followed by clustering in coated regions. J Cell Biol 82:614–625
