ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei

American Society for Microbiology - Tập 5 Số 1 - Trang 45-53 - 2006
Silvia V. Brown1, Paul Hosking1, Jinlei Li1, Noreen Williams1
1Department of Microbiology and Immunology and Witebsky Center for Microbial Pathogenesis and Immunology, 253 Biomedical Research Building, University at Buffalo, Buffalo, New York 14214

Tóm tắt

ABSTRACT The mitochondrion of Trypanosoma brucei bloodstream form maintains a membrane potential, although it lacks cytochromes and several Krebs cycle enzymes. At this stage, the ATP synthase is present at reduced, although significant, levels. To test whether the ATP synthase at this stage is important for maintaining the mitochondrial membrane potential, we used RNA interference (RNAi) to knock down the levels of the ATP synthase by targeting the F 1 -ATPase α and β subunits. RNAi-induced cells grew significantly slower than uninduced cells but were not morphologically altered. RNAi of the β subunit decreased the mRNA and protein levels for the β subunit, as well as the mRNA and protein levels of the α subunit. Similarly, RNAi of α subunit decreased the α subunit transcript and protein levels, as well as the β-subunit transcript and protein levels. In contrast, α and β RNAi knockdown resulted in a 60% increase in the F 0 complex subunit 9 protein levels without a significant change in the steady-state transcript levels of this subunit. The F 0 -32-kDa subunit protein expression, however, remained stable throughout induction of RNAi for α or β subunits. Oligomycin-sensitive ATP hydrolytic and synthetic activities were decreased by 43 and 44%, respectively. Significantly, the mitochondrial membrane potential of α and β RNAi cells was decreased compared to wild-type cells, as detected by MitoTracker Red CMXRos fluorescence microscopy and flow cytometry. These results support the role of the ATP synthase in the maintenance of the mitochondrial membrane potential in bloodstream form T. brucei .

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American Society for Microbiology

Tập 5 Số 1

45-53

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