APC protein immunoexpression in colorectal adenoma and adenocarcinoma

Journal of Coloproctology - Tập 33 - Trang 118-125 - 2013
Vivian Sati Oba Bourroul1,2, Guilherme Muniz Bourroul2, Giovanna Canato Toloi3, Rogério Tadeu Palma1,4, Celina Tizuko Fujiyama Oshima5, Thiago Simão Gomes6, Sílvia Saiuli Miki Ihara6, Jaques Waisberg7
1Service of Digestive System Surgery of Hospital do Servidor Público Estadual (HSPE), São Paulo, SP, Brazil
2Service of Digestive System Surgery of Hospital Estadual Mário Covas, Santo André, SP, Brazil
3Faculdade de Medicina do ABC, São Paulo, SP, Brazil
4Discipline of Service of Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, Brazil
5Laboratory of Molecular Pathology of Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo, SP, Brazil
6Laboratory of Molecular Pathology, Departament of Pathology, EPM-UNIFESP, São Paulo, SP, Brazil
7General and Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, Brazil

Tóm tắt

Abstract

Background activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma.

Objective detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa.

Methods 42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained.

Results the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24).

Conclusions the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism.


Tài liệu tham khảo

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Thông tin xuất bản

Journal of Coloproctology

Tập 33

118-125

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