ACTION SPECTRUM FOR INACTIVATION OF THE INFECTIVITY OF POTATO VIRUS X BY U.V. RADIATION

Photochemistry and Photobiology - Tập 10 Số 1 - Trang 53-59 - 1969
A. Kleczkowski1, D. A. Govier1
1Rothamsted Experimental Station, Harpenden, Herts., England

Tóm tắt

Abstract— Quantum yields for inactivation of infectivity of potato virus X by monochromatic ultraviolet radiation of wavelengths ranging from 230 to 290 nm, were measured with reference to energy absorbed by (a) the whole virus and (b) the virus RNA. The yields depended on the wavelength, but those with reference to energy absorbed by the RNA varied much less (with extreme values of 10‐3 and 1.9 ± 10‐3 than those with reference to whole virus. Consequently the action spectrum for inactivation of a dilute solution of the virus resembled the shape of the absorption spectrum of the RNA, but not closely enough to allow coincidence by adjusting the scales. The amount of photoreactivation increased as the wavelength increased and also as the year progressed from May to July; the extreme values of the photoreactivable sector were 0.43 and 0.86.

Từ khóa


Tài liệu tham khảo

10.1111/j.1751-1097.1965.tb05738.x

10.1099/0022-1317-1-4-441

10.1016/0042-6822(60)90147-1

10.1111/j.1751-1097.1963.tb08907.x

10.1038/183503a0

Govier D. A., 1969, J. gen. Virol.

10.1098/rspa.1956.0102

Kleczkowski A., 1968, Methods in Virology, 716

10.1139/v59-051

Loring H. S., 1938, J.Biol. Chem., 126, 455, 10.1016/S0021-9258(18)73852-0

10.1139/v59-002

10.1073/pnas.54.4.1261

10.1016/0042-6822(59)90066-2

Kleczkowski A., 1968, Methods in Virology, 137

10.1073/pnas.22.1.19